CAJ | 학술논문

目的探讨系统性红斑狼疮(SLE)患者外周全血基因组中DNA甲基化生物标记,用于SLE的病情评估.方法 58例SLE患者中重度活动14例,中度活动25例,非活动19例;健康对照组50例,亚硫酸氢盐测序法测定白介素2共同受体γc(IL-2RG)启动子区域甲基化水平,实时测定IL-2RG mRNA的表达水平.结果 ①SLE重度活动组、中度活动组、非活动组和健康对照组外周全血IL-2RG启动子区域甲基化水平分别为0.217±0.140、0.342±0.085、0.325±0.230、0.175±0.036,非活动组高于活动组和健康对照组(P＜0.01),差异有统计学意义.IL-2RG启动子区域甲基化水平SLE患者组(0.283±0.073)高于健康对照组(P＜0.05),差异有统计学意义.②SLE患者组和健康对照组外周血IL-2RG mRNA表达水平分别为2.550±0.823和4.293±1.283,健康对照组高于SLE患者组(P＜0.05),差异有统计学意义.20例SLE患者 IL-2RG mRNA表达水平与IL-2RG启动子区域甲基化水平呈负相关(r=-0.44,P＜0.05).结论 SLE患者全血基因组IL-2RG启动子区域特定CpG位点甲基化水平较健康对照组明显升高,且活动期SLE患者组与稳定期SLE患者组相比全血基因组IL-2RG启动子区域甲基化水平也有差异.
목적 탐토계통성홍반랑창(SLE)환자외주전혈기인조중DNA갑기화생물표기,용우SLE적병정평고.방법 58례SLE환자중중도활동14례,중도활동25례,비활동19례;건강대조조50례,아류산경염측서법측정백개소2공동수체γc(IL-2RG)계동자구역갑기화수평,실시측정IL-2RG mRNA적표체수평.결과 ①SLE중도활동조、중도활동조、비활동조화건강대조조외주전혈IL-2RG계동자구역갑기화수평분별위0.217±0.140、0.342±0.085、0.325±0.230、0.175±0.036,비활동조고우활동조화건강대조조(P＜0.01),차이유통계학의의.IL-2RG계동자구역갑기화수평SLE환자조(0.283±0.073)고우건강대조조(P＜0.05),차이유통계학의의.②SLE환자조화건강대조조외주혈IL-2RG mRNA표체수평분별위2.550±0.823화4.293±1.283,건강대조조고우SLE환자조(P＜0.05),차이유통계학의의.20례SLE환자 IL-2RG mRNA표체수평여IL-2RG계동자구역갑기화수평정부상관(r=-0.44,P＜0.05).결론 SLE환자전혈기인조IL-2RG계동자구역특정CpG위점갑기화수평교건강대조조명현승고,차활동기SLE환자조여은정기SLE환자조상비전혈기인조IL-2RG계동자구역갑기화수평야유차이.
Objective To investigate DNA methylation markers in the whole blood of patients with systemic lupus erythematosus(SLE),in hope to facilitate the evaluation of SLE severity.Methods Whole blood samples were obtained from 58 patients with SLE(including 14 cases of severe SLE,25 moderate SLE,19 inactive SLE)and 50 healthy controls.Bisulphite sequencing was performed to determine the methylation status of interleukin-2 common receptor gamma chain(IL-2RG)promoter region,and real-time reverse transcriptionPCR to quantify the expression level of IL-2RG mRNA,in these subjects.Results The methylation level of IL2RG promoter region was 0.217 ± 0.140,0.325 ± 0.230,0.342 ± 0.085 and 0.175 ± 0.036 in the patients withsevere,moderate and inactive SLE and healthy controls,respectively.A significant increase was observed in the methylation level of IL-2RG promoter region in the patients with inactive SLE compared with the patients with severe SLE and healthy controls(both P ＜ 0.01),and in the patients with SLE compared with the healthy controls(0.263 ± 0.047 vs.0.175 ± 0.036,P ＜ 0.05).The expression level of IL-2RG mRNA was significantly lower in the patients with SLE than in the healthy controls(2.550 ± 0.823 vs.4.293 ± 1.283,P ＜ 0.05).A negative correlation was observed between the expression level of IL-2RG mRNA and methylation level of IL2RG promoter region in 20 patients with SLE(r =-0.44,P ＜ 0.05).Conclusion The methylation status of IL2RG promoter region is statistically higher in patients with SLE than in healthy controls,and significantly different between patients with active SLE and those with stable SLE.