中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
11期
796-798
,共3页
银屑病%PML基因%RT-PCR
銀屑病%PML基因%RT-PCR
은설병%PML기인%RT-PCR
Psoriasis%PML gene%RT-PCR
目的 检测寻常性银屑病患者外周血淋巴细胞中早幼粒细胞白血病(PML)基因的表达,以探索PML基因与银屑病之间的相互关系.方法 收集年龄18～ 65岁、未经系统治疗的寻常性银屑病患者50例,健康人对照45例.分离外周血淋巴细胞,使用RNAprep pure血液总RNA提取试剂盒提取淋巴细胞RNA,用实时荧光定量逆转录聚合酶链反应检测外周血淋巴细胞PML基因mRNA的表达;流式细胞仪检测30例银屑病患者和25例健康人对照外周血淋巴细胞PML蛋白荧光强度值.分别用独立样本t检验对检测结果进行统计分析.结果 50例银屑病患者和45例健康人对照外周血淋巴细胞PML mRNA相对定量(RQ)值分别为14.98±3.64和5.50±1.10,银屑病患者组显著高于健康人对照组(两组比较,t=16.79,P＜0.05).30例银屑病患者和25例健康人对照外周血淋巴细胞PML蛋白荧光强度值分别为3.13±0.27和2.43±0.21,银屑病患者组亦显著高于健康人对照组(两组比较,t=6.93,P＜ 0.05).结论 PML基因mRNA及蛋白在银屑病患者外周血淋巴细胞中高表达,提示PML基因可能在银屑病的发病中起一定作用.
目的 檢測尋常性銀屑病患者外週血淋巴細胞中早幼粒細胞白血病(PML)基因的錶達,以探索PML基因與銀屑病之間的相互關繫.方法 收集年齡18～ 65歲、未經繫統治療的尋常性銀屑病患者50例,健康人對照45例.分離外週血淋巴細胞,使用RNAprep pure血液總RNA提取試劑盒提取淋巴細胞RNA,用實時熒光定量逆轉錄聚閤酶鏈反應檢測外週血淋巴細胞PML基因mRNA的錶達;流式細胞儀檢測30例銀屑病患者和25例健康人對照外週血淋巴細胞PML蛋白熒光彊度值.分彆用獨立樣本t檢驗對檢測結果進行統計分析.結果 50例銀屑病患者和45例健康人對照外週血淋巴細胞PML mRNA相對定量(RQ)值分彆為14.98±3.64和5.50±1.10,銀屑病患者組顯著高于健康人對照組(兩組比較,t=16.79,P＜0.05).30例銀屑病患者和25例健康人對照外週血淋巴細胞PML蛋白熒光彊度值分彆為3.13±0.27和2.43±0.21,銀屑病患者組亦顯著高于健康人對照組(兩組比較,t=6.93,P＜ 0.05).結論 PML基因mRNA及蛋白在銀屑病患者外週血淋巴細胞中高錶達,提示PML基因可能在銀屑病的髮病中起一定作用.
목적 검측심상성은설병환자외주혈림파세포중조유립세포백혈병(PML)기인적표체,이탐색PML기인여은설병지간적상호관계.방법 수집년령18～ 65세、미경계통치료적심상성은설병환자50례,건강인대조45례.분리외주혈림파세포,사용RNAprep pure혈액총RNA제취시제합제취림파세포RNA,용실시형광정량역전록취합매련반응검측외주혈림파세포PML기인mRNA적표체;류식세포의검측30례은설병환자화25례건강인대조외주혈림파세포PML단백형광강도치.분별용독립양본t검험대검측결과진행통계분석.결과 50례은설병환자화45례건강인대조외주혈림파세포PML mRNA상대정량(RQ)치분별위14.98±3.64화5.50±1.10,은설병환자조현저고우건강인대조조(량조비교,t=16.79,P＜0.05).30례은설병환자화25례건강인대조외주혈림파세포PML단백형광강도치분별위3.13±0.27화2.43±0.21,은설병환자조역현저고우건강인대조조(량조비교,t=6.93,P＜ 0.05).결론 PML기인mRNA급단백재은설병환자외주혈림파세포중고표체,제시PML기인가능재은설병적발병중기일정작용.
Objective To detect the expression of promyelocytic leukemia(PML)gene in peripheral blood lymphocytes from patients with psoriasis vulgaris,and to investigate the relationship between PML gene and psoriasis.Methods This study included 50 patients with vulgaris psoriasis who were aged between 18 and 65 years and had never received systemic treatment,as well as 45 healthy controls.Peripheral blood samples were collected from these subjects,and lymphocytes were separated by density-gradient technique.Then,RNA was extracted from the lymphocytes by using RNAprep pure blood kit followed by real time fluorescence-based quantitative reverse transcription-PCR for the detection of PML mRNA expression.Flow cytometry was performed to quantify the expression of PML protein in the lymphocytes.Independent samples t test was carried out to compare the differences in PML expression between the patients and controls.Results The relative quantity (RQ)of PML mRNA was 14.98 ± 3.64 in peripheral blood lymphocytes from the patients,significantly higher than that in the healthy controls(5.50 ± 1.10,t =16.79,P ＜ 0.05).Similarly,a significant increment was observed in the fluorescence intensity of PML protein in peripheral blood lymphocytes from the patients compared with the healthy controls(3.13 ± 0.27 vs.2.43 ± 0.21,t =6.93,P ＜ 0.05).Conclusions PML is overexpressed at the protein and mRNA levels in peripheral blood lymphocytes from patients with psoriasis vulgaris,hinting that PML may play a certain role in the development of psoriasis.
