CAJ | 학술논문

目的探讨系统性红斑狼疮(SLE)患者外周血T淋巴细胞激活状态及OX40、OX40L mRNA表达水平.方法采用双色流式细胞仪测定SLE患者和健康人外周血CD38CD3分子表达,采用荧光定量逆转录聚合酶链反应(RT-PCR)测定外周血单一核细胞(PBMC)中OX40、OX40L mRNA表达水平.结果 SLE患者外周血CD38CD3分子表达水平显著高于健康人(t=3.12,P＜0.05).活动期SLE患者PBMC OX40、OX40L mRNA水平显著高于非活动期患者和健康人(F=4.13,3.12,P值均＜0.01).OX40 mRNA水平与SLE疾病活动指数呈正相关(r=0.35,P＜0.05).结论 T淋巴细胞异常活化及OX40、OX40L mRNA表达水平上调在SLE的发生、发展中可能起一定作用.
목적 탐토계통성홍반랑창(SLE)환자외주혈T림파세포격활상태급OX40、OX40L mRNA표체수평.방법 채용쌍색류식세포의측정SLE환자화건강인외주혈CD38CD3분자표체,채용형광정량역전록취합매련반응(RT-PCR)측정외주혈단일핵세포(PBMC)중OX40、OX40L mRNA표체수평.결과 SLE환자외주혈CD38CD3분자표체수평현저고우건강인(t=3.12,P＜0.05).활동기SLE환자PBMC OX40、OX40L mRNA수평현저고우비활동기환자화건강인(F=4.13,3.12,P치균＜0.01).OX40 mRNA수평여SLE질병활동지수정정상관(r=0.35,P＜0.05).결론 T림파세포이상활화급OX40、OX40L mRNA표체수평상조재SLE적발생、발전중가능기일정작용.
Objective To evaluate the activation status of T lymphocytes and to measure the expression of OX40 and OX40L mRNA in peripheral blood from patients with systemic lupus erythematosus(SLE).Methods Peripheral blood was collected from 30 patients with SLE and 15 healthy controls.The expression of CD38 and CD3 on peripheral blood T lymphocytes were quantified by using a two-color fluorescence activated cell sorter.Fluorescence-based quantitative reverse transcription PCR was performed to determine the expression levels of OX40 and OX40L mRNA in peripheral blood mononuclear cells(PBMCs).Results The coexpression levels of CD38 and CD3 were significantly increased in patients with SLE compared with healthy controls(t =3.12,P ＜ 0.05).Increased expression levels of OX40 and OX40L mRNA were observed in patients with active SLE compared with those with inactive SLE and healthy controls(F =4.13,3.12,both P ＜ 0.01).The level of OX40 mRNA was positively correlated with SLE disease activity index(SLEDAI)score in the patients(r =0.35,P ＜ 0.05).Conclusion Abnormally activated T lymphocytes and upregulated expression of OX40 and OX40L mRNA may play a certain role in the initiation and progression of SLE.