中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
12期
862-864
,共3页
王娟%白莉%鲍海平%郑爱义%武晓华%赵一锦%罗燕%米希婷
王娟%白莉%鮑海平%鄭愛義%武曉華%趙一錦%囉燕%米希婷
왕연%백리%포해평%정애의%무효화%조일금%라연%미희정
扁平苔藓%肿瘤坏死因子α%半胱氨酸天冬氨酸蛋白酶8%半胱氨酸天冬氨酸蛋白酶3%细胞凋亡
扁平苔蘚%腫瘤壞死因子α%半胱氨痠天鼕氨痠蛋白酶8%半胱氨痠天鼕氨痠蛋白酶3%細胞凋亡
편평태선%종류배사인자α%반광안산천동안산단백매8%반광안산천동안산단백매3%세포조망
Lichen planus%Tumor necrosis factor-alpha%Caspase 8%Caspase 3%Apoptosis
目的 研究扁平苔藓皮损中肿瘤坏死因子α(TNF-α)、半胱氨酸天冬氨酸蛋白酶8(Caspase-8)和Caspase-3的表达及意义.方法 采用免疫组化法和原位末端标记技术(TUNEL)检测20例扁平苔藓患者皮损和20例健康人皮肤组织中TNF-α、Caspase-8、Caspase-3的表达和细胞凋亡情况.采用SPSS 11.5统计软件,组间比较采用成组t检验,指标间相关性采用Pearson相关分析.结果 扁平苔藓组TNF-α、Caspase-8和Caspase-3的积分光密度值分别为12580±2330、11690±3520和11450±2820,均明显高于健康对照组(分别为5120±1780、3870±3360、4760±1930),两组比较,t值分别为11.38、7.19、8.76,P值均＜0.01；扁平苔藓组角质形成细胞凋亡指数(71.35±7.93),明显高于健康对照组(33.62±8.75),两组比较,t=14.29,P＜ 0.01.扁平苔藓组TNF-α、Caspase-8的表达强度与角质形成细胞凋亡指数间均呈正相关,r分别为0.72、0.75,P值均＜0.01；同时两者与Caspase-3的阳性表达也均呈正相关,r分别为0.68、0.73,P值均＜0.01.结论 TNF-α、Caspase-8和Caspase-3的高表达可能参与了扁平苔藓中角质形成细胞的凋亡.
目的 研究扁平苔蘚皮損中腫瘤壞死因子α(TNF-α)、半胱氨痠天鼕氨痠蛋白酶8(Caspase-8)和Caspase-3的錶達及意義.方法 採用免疫組化法和原位末耑標記技術(TUNEL)檢測20例扁平苔蘚患者皮損和20例健康人皮膚組織中TNF-α、Caspase-8、Caspase-3的錶達和細胞凋亡情況.採用SPSS 11.5統計軟件,組間比較採用成組t檢驗,指標間相關性採用Pearson相關分析.結果 扁平苔蘚組TNF-α、Caspase-8和Caspase-3的積分光密度值分彆為12580±2330、11690±3520和11450±2820,均明顯高于健康對照組(分彆為5120±1780、3870±3360、4760±1930),兩組比較,t值分彆為11.38、7.19、8.76,P值均＜0.01；扁平苔蘚組角質形成細胞凋亡指數(71.35±7.93),明顯高于健康對照組(33.62±8.75),兩組比較,t=14.29,P＜ 0.01.扁平苔蘚組TNF-α、Caspase-8的錶達彊度與角質形成細胞凋亡指數間均呈正相關,r分彆為0.72、0.75,P值均＜0.01；同時兩者與Caspase-3的暘性錶達也均呈正相關,r分彆為0.68、0.73,P值均＜0.01.結論 TNF-α、Caspase-8和Caspase-3的高錶達可能參與瞭扁平苔蘚中角質形成細胞的凋亡.
목적 연구편평태선피손중종류배사인자α(TNF-α)、반광안산천동안산단백매8(Caspase-8)화Caspase-3적표체급의의.방법 채용면역조화법화원위말단표기기술(TUNEL)검측20례편평태선환자피손화20례건강인피부조직중TNF-α、Caspase-8、Caspase-3적표체화세포조망정황.채용SPSS 11.5통계연건,조간비교채용성조t검험,지표간상관성채용Pearson상관분석.결과 편평태선조TNF-α、Caspase-8화Caspase-3적적분광밀도치분별위12580±2330、11690±3520화11450±2820,균명현고우건강대조조(분별위5120±1780、3870±3360、4760±1930),량조비교,t치분별위11.38、7.19、8.76,P치균＜0.01；편평태선조각질형성세포조망지수(71.35±7.93),명현고우건강대조조(33.62±8.75),량조비교,t=14.29,P＜ 0.01.편평태선조TNF-α、Caspase-8적표체강도여각질형성세포조망지수간균정정상관,r분별위0.72、0.75,P치균＜0.01；동시량자여Caspase-3적양성표체야균정정상관,r분별위0.68、0.73,P치균＜0.01.결론 TNF-α、Caspase-8화Caspase-3적고표체가능삼여료편평태선중각질형성세포적조망.
Objective To quantify the expressions of tumor necrosis factor-α (TNF-α),caspase-8 and caspase-3 in lichen planus (LP) lesions,and to investigate their significance.Methods Skin samples were collected from the lesions of 20 patients with LP and normal skin of 20 healthy human controls.Immunohistochemistry was used to determine the expressions of TNF-αt,caspase-8 and caspase-3,and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) technique to evaluate the apoptosis in keratinocytes,in these samples.Results The expression levels (expressed in integrated optical density,IOD)of TNF-α,caspase-8 and caspase-3 were (12.58 ± 2.33) × 103,(11.69 ± 3.52) × 103 and (11.45 ± 2.82) × 103 respectively in LP lesions,significantly higher than those in the normal skin ((5.12 ± 1.78) × 103,(3.87 ± 3.36)× 103,(4.76 ± 1.93) × 103,t =11.38,7.19,8.76,respectively,all P ＜ 0.01).Elevated apoptosis index was noted in keratinocytes from LP lesions compared with those from normal skin (71.35 ± 7.93 vs.33.62 ± 8.75,t =14.29,P ＜ 0.01).In LP lesions,the expressions of both TNF-α and caspase-8 were positively correlated with the apoptosis index of keratinocytes (r =0.72,0.75,respectively,both P ＜ 0.01) and the expression of caspase-3 (r =0.68,0.73,respectively,both P ＜ 0.01).Conclusion The up-regulated expressions of TNF-α,caspase-8 and caspase-3 may participate in the apoptosis in keratinocytes in LP.
