CAJ | 학술논문

目的探讨CD200在斑秃患者外周血淋巴细胞和头皮毛囊上的表达.方法用流式细胞仪检测CD200在43例斑秃患者外周血淋巴细胞上的表达.免疫组化法检测CD200和正常毛囊外根鞘基底层标记CK15(cytokeratin 15)在8例斑秃患者毛囊上的表达,以同等例数健康人的标本做对照,进行分析比较.结果 CD200在斑秃患者外周血总淋巴细胞上及T淋巴细胞上的表达率分别为5.73％±3.46％,8.85％±4.8％,明显低于健康对照人群(12.01％±4.90％;12.31％±3.12％,t值分别为6.865,3.964,P值均＜0.05；而在患者B淋巴细胞上的表达比例为74.68±8.12,较健康对照人群差异无统计学意义(75.75±9.45,t=0.570,P＞0.05)；斑秃患者毛囊上CD200的表达较健康对照人群亦明显下降(P＜0.05),而CK15较健康对照无明显下降.结论 CD200在斑秃患者外周血淋巴细胞和毛囊上表达的下降可能参与了斑秃的发病.
목적 탐토CD200재반독환자외주혈림파세포화두피모낭상적표체.방법 용류식세포의검측CD200재43례반독환자외주혈림파세포상적표체.면역조화법검측CD200화정상모낭외근초기저층표기CK15(cytokeratin 15)재8례반독환자모낭상적표체,이동등례수건강인적표본주대조,진행분석비교.결과 CD200재반독환자외주혈총림파세포상급T림파세포상적표체솔분별위5.73％±3.46％,8.85％±4.8％,명현저우건강대조인군(12.01％±4.90％;12.31％±3.12％,t치분별위6.865,3.964,P치균＜0.05；이재환자B림파세포상적표체비례위74.68±8.12,교건강대조인군차이무통계학의의(75.75±9.45,t=0.570,P＞0.05)；반독환자모낭상CD200적표체교건강대조인군역명현하강(P＜0.05),이CK15교건강대조무명현하강.결론 CD200재반독환자외주혈림파세포화모낭상표체적하강가능삼여료반독적발병.
Objective To investigate the expression of CD200 in peripheral lymphocytes and hair follicles from patients with alopecia areata (AA).Methods Flow cytometry was used to detect the expression of CD200 in peripheral lymphocytes from 43 patients with AA and 43 healthy controls.Immunohistochemistry was carried out to quantify the expression of CD200 and cytokeratin 15(CK15,a marker for basal cells of the outer root sheath) in resected scalp specimens from 8 patients with AA and 8 healthy controls.Differences in the expression of CD200 and CK15 between the patients and controls were assessed by independent-samples t test and rank sum test.Data were processed by the software SPSS17.0.Results The percentage of CD200-expressing cells in peripheral blood lymphocytes and T lymphocytes was significantly lower in the patients with AA than in the healthy controls (5.73％ ± 3.46％ vs.12.01％ ± 4.90％,8.85％ ± 4.80％ vs.12.31％ ± 3.12％,t =6.865,3.964,respectively,both P ＜ 0.05).However,no significant difference was observed in the percentage of CD200-expressing cells in peripheral blood B lymphocytes between the patients and controls (74.68％ ± 8.12％ vs.75.75％ ± 9.45％,t =0.570,P ＞ 0.05).Further more,the patients showed a lower expression of CD200 (P ＜ 0.05),but a similar expression of CK15 (P ＞ 0.05) in hair follicles compared with the controls.Conclusion The decrease in CD200 expression in peripheral lymphocytes and hair follicles may be involved in the pathogenesis of AA.