CAJ | 학술논문

目的探讨儿童特应性皮炎血清和CD4+CD25+T分泌细胞因子IL-IO表达,分析其与病程及严重程度的相关性.方法特应性皮炎患儿按SCORAD指数分3组,轻度10例、中度16例、重度20例.抽取46例特应性皮炎患儿和31例健康对照儿外周血,用免疫磁珠分离获得CD4+CD25+T细胞,用ELISA法分别检测患病组和健康对照组血清及CD4+CD25+T细胞培养液IL-10水平,并分析IL-10水平与SCORAD评分的相关性.结果轻、中、重度AD各组血清IL-10水平分别为(43.10±25.07)、(68.40±36.65)、(55.55±41.97) pg/ml,与健康对照组(58.27±36.84) pg/ml比较,差异均无统计学意义(P＞0.05),与患儿SCORAD评分无相关性.轻、中、重度AD组CD4+CD25+T细胞分泌IL-10含量分别为(52.96±11.69)、(49.86±9.18)、(27.25±7.01) pg/ml,重度AD组低于健康对照组(55.15±11.15) pg/ml (P＜ 0.05);轻、中度组与健康对照组差异无统计学意义(P＞0.05).CD4+CD25+T细胞分泌IL-10的水平与患儿疾病严重程度SCORAD评分呈明显负相关(r值分别为-0.757,P＜0.0l).结论 CD4+CD25+T细胞及相关因子IL-10可能参与儿童特应性皮炎的发病.
목적 탐토인동특응성피염혈청화CD4+CD25+T분비세포인자IL-IO표체,분석기여병정급엄중정도적상관성.방법 특응성피염환인안SCORAD지수분3조,경도10례、중도16례、중도20례.추취46례특응성피염환인화31례건강대조인외주혈,용면역자주분리획득CD4+CD25+T세포,용ELISA법분별검측환병조화건강대조조혈청급CD4+CD25+T세포배양액IL-10수평,병분석IL-10수평여SCORAD평분적상관성.결과 경、중、중도AD각조혈청IL-10수평분별위(43.10±25.07)、(68.40±36.65)、(55.55±41.97) pg/ml,여건강대조조(58.27±36.84) pg/ml비교,차이균무통계학의의(P＞0.05),여환인SCORAD평분무상관성.경、중、중도AD조CD4+CD25+T세포분비IL-10함량분별위(52.96±11.69)、(49.86±9.18)、(27.25±7.01) pg/ml,중도AD조저우건강대조조(55.15±11.15) pg/ml (P＜ 0.05);경、중도조여건강대조조차이무통계학의의(P＞0.05).CD4+CD25+T세포분비IL-10적수평여환인질병엄중정도SCORAD평분정명현부상관(r치분별위-0.757,P＜0.0l).결론 CD4+CD25+T세포급상관인자IL-10가능삼여인동특응성피염적발병.
Objective To determine the level of interleukin (IL)-10 in sera and culture supernatants of CD4+CD25+T cells from children with atopic dermatitis (AD),and to evaluate its relationship with clinical course and severity of AD.Methods Totally,46 children with AD and 31 healthy controls were included in the study.All the patients were divided into 3 groups,i.e.,mild (n =10),moderate (n =16) and severe (n =20) group,according to severity scoring of atopic dermatitis (SCORAD) score.Venous blood samples were obtained from the patients and healthy controls.CD4+CD25+ regulatory T cells were separated from the blood samples by magnetic cell sorting (MACS) system in two steps and cultured in vitro.Enzyme linked immunosorbent assay (ELISA) was conducted to quantify the level of IL-10 in sera and culture supernatants of CD4+CD25 + T cells from these subjects.Analysis of variance was carried out to compare the level of supematant and serum IL-10 between the patients and controls,and Pearson correlation analysis to assess the relationship between the level of IL-10 and SCORAD score.Results The patients with mild,moderate and severe AD showed a similar serum IL-10 level compared with the healthy controls ((43.10 ± 25.07) pg/ml,(68.40 ± 36.65) pg/ml and (55.55 ± 41.97) pg/ml vs.(58.27 ± 36.84) pg/ml,all P ＞ 0.05).The level of supernatant IL-10 secreted by CD4+CD25+ T cells from the controls was significantly higher than that from the patients with severe AD ((55.15 ± 11.15) pg/ml vs.(27.25 ± 7.01) pg/ml,P ＜ 0.05),but similar to that from the patients with mild and moderate AD ((52.96 ± 11.69) pg/ml and (49.86 ± 9.18) pg/ml,respectively,both P ＞ 0.05).The level of secreted IL-10 was negatively correlated with SCORAD score (r =－0.757,P ＜ 0.01),whereas the serum level of IL-10 showed no statistical correlation with SCORAD score.Conclusion CD4+CD25+ T cells and IL-10 may be implicated in the development of AD.