中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
1期
37-39
,共3页
侯麦花%卢新政%Peter M Elias
侯麥花%盧新政%Peter M Elias
후맥화%로신정%Peter M Elias
黑素细胞%角质形成细胞%半胱氨酸天冬氨酸蛋白酶14%人%肤色
黑素細胞%角質形成細胞%半胱氨痠天鼕氨痠蛋白酶14%人%膚色
흑소세포%각질형성세포%반광안산천동안산단백매14%인%부색
Melanocytes%Keratinocytes%Caspase 14%Persons%Skin color
目的 探讨不同肤色人群角质形成细胞中Caspase14表达变化,明确黑素细胞和(或)黑素对其表达的影响.方法 以Western印迹检测不同肤色个体原代角质形成细胞Caspase14的表达.取体外培养不同肤色的2代角质形成细胞,分别加人不同肤色的黑素细胞在分化培养基下共同培养24 h,以不加黑素细胞组为对照,Western印迹检测Caspase14的表达.结果 浅、深肤色个体包皮原代角质形成细胞Caspase 14的表达存在显著差异,深肤色者(Fitzpatrick Ⅳ/Ⅴ)原代角质形成细胞中Caspase14的表达显著高于浅肤色者(Fitzpatrick Ⅰ/Ⅱ)(P＜0.01)；深、浅肤色个体的角质形成细胞在与不同肤色个体的黑素细胞在分化培养基中共培养后24 h后,检测发现,与对照组相比,浅肤色个体角质形成细胞Caspase14表达的增加,差异无统计学意义(P＞0.05),而深肤色个体角质形成细胞Caspase14的表达则显著上调(P＜0.05).结论 深肤色个体角质形成细胞Caspase14表达显著高于浅肤色个体者.黑素细胞显著增加深肤色者角质形成细胞中Caspase 14水平的表达.
目的 探討不同膚色人群角質形成細胞中Caspase14錶達變化,明確黑素細胞和(或)黑素對其錶達的影響.方法 以Western印跡檢測不同膚色箇體原代角質形成細胞Caspase14的錶達.取體外培養不同膚色的2代角質形成細胞,分彆加人不同膚色的黑素細胞在分化培養基下共同培養24 h,以不加黑素細胞組為對照,Western印跡檢測Caspase14的錶達.結果 淺、深膚色箇體包皮原代角質形成細胞Caspase 14的錶達存在顯著差異,深膚色者(Fitzpatrick Ⅳ/Ⅴ)原代角質形成細胞中Caspase14的錶達顯著高于淺膚色者(Fitzpatrick Ⅰ/Ⅱ)(P＜0.01)；深、淺膚色箇體的角質形成細胞在與不同膚色箇體的黑素細胞在分化培養基中共培養後24 h後,檢測髮現,與對照組相比,淺膚色箇體角質形成細胞Caspase14錶達的增加,差異無統計學意義(P＞0.05),而深膚色箇體角質形成細胞Caspase14的錶達則顯著上調(P＜0.05).結論 深膚色箇體角質形成細胞Caspase14錶達顯著高于淺膚色箇體者.黑素細胞顯著增加深膚色者角質形成細胞中Caspase 14水平的錶達.
목적 탐토불동부색인군각질형성세포중Caspase14표체변화,명학흑소세포화(혹)흑소대기표체적영향.방법 이Western인적검측불동부색개체원대각질형성세포Caspase14적표체.취체외배양불동부색적2대각질형성세포,분별가인불동부색적흑소세포재분화배양기하공동배양24 h,이불가흑소세포조위대조,Western인적검측Caspase14적표체.결과 천、심부색개체포피원대각질형성세포Caspase 14적표체존재현저차이,심부색자(Fitzpatrick Ⅳ/Ⅴ)원대각질형성세포중Caspase14적표체현저고우천부색자(Fitzpatrick Ⅰ/Ⅱ)(P＜0.01)；심、천부색개체적각질형성세포재여불동부색개체적흑소세포재분화배양기중공배양후24 h후,검측발현,여대조조상비,천부색개체각질형성세포Caspase14표체적증가,차이무통계학의의(P＞0.05),이심부색개체각질형성세포Caspase14적표체칙현저상조(P＜0.05).결론 심부색개체각질형성세포Caspase14표체현저고우천부색개체자.흑소세포현저증가심부색자각질형성세포중Caspase 14수평적표체.
Objective To compare the expression of caspase 14 in keratinocytes from people of different skin color,and to evaluate the effect of melanocytes and/or melanin on the expression of caspase 14.Methods Epidermal keratinocytes and melanocytes were isolated from the foreskin of fair-and dark-skinned neonates,and subjected to a primary culture.The second-passage keratinocytes were divided into 6 groups to be cultured with or without the presence of melanocytes from individuals with fair or dark skin at a ratio of 10 ∶ 1 for 24 hours.Western blot was performed to measure the expression of caspase 14 in these keratinocytes.The difference in caspase 14 expression between keratinocytes receiving different treatment was assessed by t test and analysis of variance (ANOVA).Results The expression of caspase 14 was significantly higher in keratinocytes from darkskinned(Fitzpatrick skin type Ⅳ/Ⅴ) individuals than in fair-skinned (Fitzpatrick skin type Ⅰ/Ⅱ) individuals(P ＜ 0.01).The cocuhure with melanocytes elevated the expression of caspase 14 in second-passage keratinocytes,and the elevation was statistically significant in keratinocytes from dark-skinned individuals cocultured with melanocytes from dark-skinned individuals (P ＜ 0.05),but insignificant in keratinocytes from fair-skinned individuals cocultured with melanocytes from dark-or fair-skinned individuals or in keratinocytes from darkskinned individuals cocultured with melanocytes from fair-skinned individuals (all P ＞ 0.05).Conclusions The expression of caspase 14 is higher in keratinocytes from dark-skinned individuals than in those from fair-skinned individuals,and melanocytes can enhance the expression of caspase 14 in keratinocytes from dark-skinned individuals.
