中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
1期
58-60
,共3页
目的 探讨马齿苋提取物对肿瘤坏死因子α(TNF-α)、细胞间黏附分子1(ICAM-1)表达的影响,初步阐明马齿苋抗炎机制.方法 建立二甲苯致小鼠耳廓肿胀炎症模型,并分为4组.空白组:不采用任何干预措施,阴性对照组:建立模型后,不加干预,阳性组:模型建立后,使用韩国进口马齿苋外涂小鼠致炎处,实验组:建立模型后,使用云南马齿苋提取物涂于致炎处.组织病理及免疫组化观察TNF-α、ICAM-1的变化并比较各组的抗炎效果.结果组织病理:空白组:真皮可见血管及少量淋巴细胞；阴性对照组:真皮组织疏松、水肿及大量淋巴细胞浸润；阳性组:轻度水肿,真皮血管周围可见部分炎症细胞浸润；实验组:HE染色:真皮轻度水肿,血管周围可见部分炎症细胞浸润.免疫组化:空白组:TNF-α、ICAM-1未见表达；阴性对照组:TNF-α强阳性表达于血管内皮细胞膜,ICAM-1强阳性表达于血管内皮细胞膜及淋巴细胞膜；阳性组:TNF-α阳性表达于血管内皮细胞膜,ICAM-1阳性表达于血管内皮细胞膜及淋巴细胞膜；实验组:TNF-α阳性表达于血管内皮细胞膜,ICAM-1阳性表达于血管内皮细胞膜及淋巴细胞膜.秩和检验:实验组与空白组比较TNF-α、ICAM-1,P值均＜0.01,差异有统计学意义.结论 云南马齿苋抗炎机制可能与影响TNF-α、ICAM-1的表达有关.
目的 探討馬齒莧提取物對腫瘤壞死因子α(TNF-α)、細胞間黏附分子1(ICAM-1)錶達的影響,初步闡明馬齒莧抗炎機製.方法 建立二甲苯緻小鼠耳廓腫脹炎癥模型,併分為4組.空白組:不採用任何榦預措施,陰性對照組:建立模型後,不加榦預,暘性組:模型建立後,使用韓國進口馬齒莧外塗小鼠緻炎處,實驗組:建立模型後,使用雲南馬齒莧提取物塗于緻炎處.組織病理及免疫組化觀察TNF-α、ICAM-1的變化併比較各組的抗炎效果.結果組織病理:空白組:真皮可見血管及少量淋巴細胞；陰性對照組:真皮組織疏鬆、水腫及大量淋巴細胞浸潤；暘性組:輕度水腫,真皮血管週圍可見部分炎癥細胞浸潤；實驗組:HE染色:真皮輕度水腫,血管週圍可見部分炎癥細胞浸潤.免疫組化:空白組:TNF-α、ICAM-1未見錶達；陰性對照組:TNF-α彊暘性錶達于血管內皮細胞膜,ICAM-1彊暘性錶達于血管內皮細胞膜及淋巴細胞膜；暘性組:TNF-α暘性錶達于血管內皮細胞膜,ICAM-1暘性錶達于血管內皮細胞膜及淋巴細胞膜；實驗組:TNF-α暘性錶達于血管內皮細胞膜,ICAM-1暘性錶達于血管內皮細胞膜及淋巴細胞膜.秩和檢驗:實驗組與空白組比較TNF-α、ICAM-1,P值均＜0.01,差異有統計學意義.結論 雲南馬齒莧抗炎機製可能與影響TNF-α、ICAM-1的錶達有關.
목적 탐토마치현제취물대종류배사인자α(TNF-α)、세포간점부분자1(ICAM-1)표체적영향,초보천명마치현항염궤제.방법 건립이갑분치소서이곽종창염증모형,병분위4조.공백조:불채용임하간예조시,음성대조조:건립모형후,불가간예,양성조:모형건립후,사용한국진구마치현외도소서치염처,실험조:건립모형후,사용운남마치현제취물도우치염처.조직병리급면역조화관찰TNF-α、ICAM-1적변화병비교각조적항염효과.결과조직병리:공백조:진피가견혈관급소량림파세포；음성대조조:진피조직소송、수종급대량림파세포침윤；양성조:경도수종,진피혈관주위가견부분염증세포침윤；실험조:HE염색:진피경도수종,혈관주위가견부분염증세포침윤.면역조화:공백조:TNF-α、ICAM-1미견표체；음성대조조:TNF-α강양성표체우혈관내피세포막,ICAM-1강양성표체우혈관내피세포막급림파세포막；양성조:TNF-α양성표체우혈관내피세포막,ICAM-1양성표체우혈관내피세포막급림파세포막；실험조:TNF-α양성표체우혈관내피세포막,ICAM-1양성표체우혈관내피세포막급림파세포막.질화검험:실험조여공백조비교TNF-α、ICAM-1,P치균＜0.01,차이유통계학의의.결론 운남마치현항염궤제가능여영향TNF-α、ICAM-1적표체유관.
Objective To explore the anti-inflammatory mechanisms of purslane by evaluating its effects on the expression of tumor necrosis factor-α (TNF-α) and intercellular adhesion molecule 1 (ICAM-1).Methods A model of inflammation was developed in 45 mice by painting xylene to the auricle of the right ears,which were then divided into 3 groups to receive no treatment (negative control group),be topically treated with the extraction of purslane from South Korea (positive control group) or Yunnan province (experimental group).Fifteen mice receiving no sensitization nor treatment served as the blank control group.Two hours after the single topical treatment,skin tissue samples were obtained from the site of experimental inflammation and subjected to pathological examination by using hematoxylin and eosin (HE) staining.Immunohistochemistry was performed to quantify the expression of TNF-α and ICAM-1 in the tissue samples.Results Pathological examination showed blood vessels and a small quantity of lymphocytes in murine dermis of the blank control group as well as loose and edematous dermis infiltrated with massive lymphocytes in the negative control group.However,there was only mild edema and perivascular infiltration with some inflammatory cells in the dermis of the positive control group and experimental group.Neither TNF-α nor ICAM-1 was expressed in the skin tissue of the blank control group,but an intense expression was observed for TNF-α in the vascular endothelial cell membrane and for ICAM-1 in the vascular endothelial cell membrane and lymphocyte membrane in the negative control group,which was significantly downregulated by the purslane from South Korea in the positive control group and by the purslane from Yunnan province in the experimental group (all P ＜ 0.01).Rank sum test showed a statistical difference in the expression level of TNF-α and ICAM-1 between the blank control group and experimental group (both P ＜0.01).Conclusion The purslane from Yunnan province may counteract inflammation by affecting the expression of TNF-α and ICAM-1.
