中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
2期
84-87
,共4页
杨晓红%曹毅%刘改荣%代群%解凡%李园园%陈微
楊曉紅%曹毅%劉改榮%代群%解凡%李園園%陳微
양효홍%조의%류개영%대군%해범%리완완%진미
银屑病%肿瘤坏死因子-α%姜黄素
銀屑病%腫瘤壞死因子-α%薑黃素
은설병%종류배사인자-α%강황소
Psoriasis%Tumor necrosis factor-alpha%Curcumin
目的 探讨姜黄素对肿瘤坏死因子(TNF)-α诱导的HaCaT细胞增殖及凋亡的影响.方法 MTT法检测不同浓度重组TNF-α(0、1、5、10、25、50、100 ng/ml)、姜黄素20μmol/L对HaCaT细胞增殖的影响.蛋白印迹实验检测25 ng/ml TNF-α和20 μmol/L姜黄素作用下HaCaT细胞PCNA、Notch-1的蛋白表达.使用Annexin-V/PI试剂盒在流式细胞仪上检测25 ng/ml TNF-α和20 μmol/L姜黄素作用下HaCaT细胞早期凋亡情况.结果 TNF-α呈浓度依赖性促进HaCaT细胞增殖,25 ng/ml TNF-α对HaCaT细胞的促增殖基本达到最大,20 μmol/L姜黄素有效抑制25 ng/ml TNF-α对HaCaT细胞的促增殖作用,25 ng/ml TNF-α对HaCaT细胞无明显促凋亡作用,而20 μmol/L姜黄素有效促进HaCaT细胞发生凋亡,并可激发25 ng/mlTNF-α对HaCaT细胞的凋亡诱导作用.结论 姜黄素可激发TNF-α对HaCaT细胞的促凋亡作用,同时抑制TNF-α对HaCaT细胞促增殖作用.
目的 探討薑黃素對腫瘤壞死因子(TNF)-α誘導的HaCaT細胞增殖及凋亡的影響.方法 MTT法檢測不同濃度重組TNF-α(0、1、5、10、25、50、100 ng/ml)、薑黃素20μmol/L對HaCaT細胞增殖的影響.蛋白印跡實驗檢測25 ng/ml TNF-α和20 μmol/L薑黃素作用下HaCaT細胞PCNA、Notch-1的蛋白錶達.使用Annexin-V/PI試劑盒在流式細胞儀上檢測25 ng/ml TNF-α和20 μmol/L薑黃素作用下HaCaT細胞早期凋亡情況.結果 TNF-α呈濃度依賴性促進HaCaT細胞增殖,25 ng/ml TNF-α對HaCaT細胞的促增殖基本達到最大,20 μmol/L薑黃素有效抑製25 ng/ml TNF-α對HaCaT細胞的促增殖作用,25 ng/ml TNF-α對HaCaT細胞無明顯促凋亡作用,而20 μmol/L薑黃素有效促進HaCaT細胞髮生凋亡,併可激髮25 ng/mlTNF-α對HaCaT細胞的凋亡誘導作用.結論 薑黃素可激髮TNF-α對HaCaT細胞的促凋亡作用,同時抑製TNF-α對HaCaT細胞促增殖作用.
목적 탐토강황소대종류배사인자(TNF)-α유도적HaCaT세포증식급조망적영향.방법 MTT법검측불동농도중조TNF-α(0、1、5、10、25、50、100 ng/ml)、강황소20μmol/L대HaCaT세포증식적영향.단백인적실험검측25 ng/ml TNF-α화20 μmol/L강황소작용하HaCaT세포PCNA、Notch-1적단백표체.사용Annexin-V/PI시제합재류식세포의상검측25 ng/ml TNF-α화20 μmol/L강황소작용하HaCaT세포조기조망정황.결과 TNF-α정농도의뢰성촉진HaCaT세포증식,25 ng/ml TNF-α대HaCaT세포적촉증식기본체도최대,20 μmol/L강황소유효억제25 ng/ml TNF-α대HaCaT세포적촉증식작용,25 ng/ml TNF-α대HaCaT세포무명현촉조망작용,이20 μmol/L강황소유효촉진HaCaT세포발생조망,병가격발25 ng/mlTNF-α대HaCaT세포적조망유도작용.결론 강황소가격발TNF-α대HaCaT세포적촉조망작용,동시억제TNF-α대HaCaT세포촉증식작용.
Objective To evaluate the effect of curcumin on the proliferation of and apoptosis in HaCaT cells induced by tumor necrosis factor α (TNF-α).Methods HaCaT cells were cultured with the presence of different concentrations (0,1,5,10,25,50,100 ng/ml) of recombinant TNF-α,curcumin of 20 μmol/L,or the combination of recombinant TNF-α (25 ng/ml) and curcumin (20 μmol/L),for 24 hours followed by the determination of cell proliferation with methyl thiazolyl tetrazolium (MTT) assay.Western blot was conducted to measure the protein expression of proliferating cell nuclear antigen (PCNA) and Notch-1 in HaCaT cells treated with recombinant TNF-α (25 ng/ml) and curcumin (20 μ mol/L) alone or in combination for 24 hours.Flow cytometry using annexin-V/propidium iodine (PI) was performed to assess the early apoptosis in HaCaT cells incubated with recombinant TNF-α of 25 ng/ml and curcumin of 20 μmol/L alone or in combination for 12 hours.Statistical analysis was carried out with one-way analysis of variance.Results Recombinant TNF-α promoted the proliferation of HaCaT cells in a dose-dependent manner,with the maximum proliferation activity observed in HaCaT cells treated with TNF-α of 25 ng/ml,while curcumin of 20 μmol/L effectively inhibited the proliferation of HaCaT cells induced by TNF-α of 25 ng/ml (P ＜ 0.01).TNF-α of 25 ng/ml had no obvious effect on cell apoptosis,while curcumin of 20 μ mol/L markedly induced the apoptosis in HaCaT cells,and there was a synergy between TNF-α of 25 ng/ml and curcumin of 20 μmol/L in the induction of apoptosis in HaCaT cells,with the apoptosis rate being 2.3％,3.4％,11.6％ and 16.8％ respectively in untreated cells,cells treated with TNF-α,curcumin,and the combination of TNF-α and curcumin,respectively.Conclusions Curcumin could enhance the inductive effect of TNF-α on the apoptosis in,but suppress the promotive effect of TNF-α on the proliferation of,HaCaT cells.
