中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
9期
633-635
,共3页
张峻岭%于旺%Youwen Zhou%马秀亮%索丹凤%闫翠彦
張峻嶺%于旺%Youwen Zhou%馬秀亮%索丹鳳%閆翠彥
장준령%우왕%Youwen Zhou%마수량%색단봉%염취언
白癜风%CLEC2B mRNA%基因表达
白癜風%CLEC2B mRNA%基因錶達
백전풍%CLEC2B mRNA%기인표체
Vitiligo%CLEC2B mRNA%Gene expression
目的 探讨CLEC2B(C-type lectin domain family 2,member B)基因在白癜风发病中的作用.方法 实时荧光定量PCR方法检测白癜风患者皮损区及外周血中CLEC2B mRNA表达,比较不同临床类型、病期、病程CLEC2B mRNA的差异表达.结果 白癜风患者组皮损中,CLEC2B mRNA表达量高于健康对照组(两组表达量分别为1.21±0.03,1.00),差异有统计学意义(t=4.432,P<0.05).白癜风患者与健康对照组外周血CLEC2B mRNA表达量比较,差异无统计学意义(两组表达量分别为1.02±0.05,1.00;t=1.435,P> 0.05).寻常型白癜风皮损组中,CLEC2B mRNA表达量高于节段型白癜风组(1.21±0.03,1.02±0.01),差异有统计学意义(t=5.330,P<0.05).不同病程白癜风患者皮损中,CLEC2B mRNA表达量比较,差异无统计学意义(P>0.05).进展期白癜风患者组皮损中CLEC2B mRNA表达量高于稳定期患者组(1.25±0.05比1.08±0.03),差异有统计学意义(t=3.046,P< 0.05).结论 CLEC2B mRNA的差异表达可能参与白癜风的发病.
目的 探討CLEC2B(C-type lectin domain family 2,member B)基因在白癜風髮病中的作用.方法 實時熒光定量PCR方法檢測白癜風患者皮損區及外週血中CLEC2B mRNA錶達,比較不同臨床類型、病期、病程CLEC2B mRNA的差異錶達.結果 白癜風患者組皮損中,CLEC2B mRNA錶達量高于健康對照組(兩組錶達量分彆為1.21±0.03,1.00),差異有統計學意義(t=4.432,P<0.05).白癜風患者與健康對照組外週血CLEC2B mRNA錶達量比較,差異無統計學意義(兩組錶達量分彆為1.02±0.05,1.00;t=1.435,P> 0.05).尋常型白癜風皮損組中,CLEC2B mRNA錶達量高于節段型白癜風組(1.21±0.03,1.02±0.01),差異有統計學意義(t=5.330,P<0.05).不同病程白癜風患者皮損中,CLEC2B mRNA錶達量比較,差異無統計學意義(P>0.05).進展期白癜風患者組皮損中CLEC2B mRNA錶達量高于穩定期患者組(1.25±0.05比1.08±0.03),差異有統計學意義(t=3.046,P< 0.05).結論 CLEC2B mRNA的差異錶達可能參與白癜風的髮病.
목적 탐토CLEC2B(C-type lectin domain family 2,member B)기인재백전풍발병중적작용.방법 실시형광정량PCR방법검측백전풍환자피손구급외주혈중CLEC2B mRNA표체,비교불동림상류형、병기、병정CLEC2B mRNA적차이표체.결과 백전풍환자조피손중,CLEC2B mRNA표체량고우건강대조조(량조표체량분별위1.21±0.03,1.00),차이유통계학의의(t=4.432,P<0.05).백전풍환자여건강대조조외주혈CLEC2B mRNA표체량비교,차이무통계학의의(량조표체량분별위1.02±0.05,1.00;t=1.435,P> 0.05).심상형백전풍피손조중,CLEC2B mRNA표체량고우절단형백전풍조(1.21±0.03,1.02±0.01),차이유통계학의의(t=5.330,P<0.05).불동병정백전풍환자피손중,CLEC2B mRNA표체량비교,차이무통계학의의(P>0.05).진전기백전풍환자조피손중CLEC2B mRNA표체량고우은정기환자조(1.25±0.05비1.08±0.03),차이유통계학의의(t=3.046,P< 0.05).결론 CLEC2B mRNA적차이표체가능삼여백전풍적발병.
Objective To evaluate the role of C-type lectin domain family 2,member B (CLEC2B) gene in the pathogenesis of vitiligo.Methods Real time fluorescence-based PCR was performed to detect the expression of CLEC2B mRNA in the peripheral blood and lesional skin of 37 patients with vitiligo as well as in the peripheral blood and normal skin of 40 healthy controls.Data were statistically analyzed by t test and chisquare test.Results Among the 37 patients,23 had progressive vitiligo,14 stable vitiligo,31 vitiligo vulgaris,6 segmental vitiligo.The expression level of CLEC2B mRNA was significantly higher in vitiligo lesions than in the control skin (1.21 ± 0.03 vs.1.00,t =4.432,P < 0.05),but was of no significant difference in peripheral blood between the patients and healthy controls (1.02 ± 0.05 vs.1.00,t =1.435,P > 0.05).Increased expression of CLEC2B mRNA was noted in lesions of vulgaris vitiligo compared with those of segmental vitiligo (1.21 ± 0.03 vs.1.02 ± 0.01,t =5.330,P < 0.05),as well as in lesions of progressive vitiligo compared with those of stable vitiligo (1.25 ± 0.05 vs.1.08 ± 0.03,t =3.046,P < 0.05).No significant difference was observed in the expression of CLEC2B mRNA among lesions of vitiligo with different courses (P > 0.05).Conclusion The differential expression of CLEC2B mRNA may take part in the pathogenesis of vitiligo.