中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
9期
640-643
,共4页
王爱民%陆捷洁%许玉军%乔凤%陆玉珠%杨先旭%张明%吴伟伟%刘巧
王愛民%陸捷潔%許玉軍%喬鳳%陸玉珠%楊先旭%張明%吳偉偉%劉巧
왕애민%륙첩길%허옥군%교봉%륙옥주%양선욱%장명%오위위%류교
银屑病%Toll样受体4%NF-κBp65%CCL20
銀屑病%Toll樣受體4%NF-κBp65%CCL20
은설병%Toll양수체4%NF-κBp65%CCL20
Psoriasis%Toll-like receptor 4%NF-κBp65%CCL20
目的 探讨Toll样受体4(TLR4)、核因子(NF)-κBp65、CC趋化因子配体20(CCL20)在银屑病患者皮损区及非皮损区的表达及意义.方法 用免疫组化EnVision法检测40例进行期银屑病患者斑块状皮损及20例进行期银屑病患者非皮损区皮肤、10例正常皮肤石蜡标本中的TLR4、NF-κBp65、CCL20的表达,对其在皮损中的表达水平进行相关性分析.结果 银屑病皮损区角质形成细胞中TLR4、NF-κBp65、CCL20的表达(A值分别为0.3006±0.1200、0.4551±0.1572和0.3862±0.1304)较正常人皮肤TLR4、NF-κBp65、CCL20的表达(A值分别为0.1379±0.0663、0.1970±0.0679和0.1262±0.0612)及银屑病非皮损区组(A值分别为0.1930±0.0687、0.2626±0.0606和0.2387±0.0467)明显上调(t值分别为4.113、5.044、6.106、3.711、5.265和4.889,均P＜0.05).与正常人皮肤相比,银屑病非皮损区TLR4、NF-κBp65、CCL20表达明显上调(t值分别为2.118、2.685和5.608,均P＜0.05).银屑病患者TLR4、NF-κBp65、CCL20的表达水平间均呈正相关(r值分别为0.766、0.851和0.885,均P＜0.05).结论 TLR4、NF-κBp65、CCL20的高表达可能与银屑病发病有关.
目的 探討Toll樣受體4(TLR4)、覈因子(NF)-κBp65、CC趨化因子配體20(CCL20)在銀屑病患者皮損區及非皮損區的錶達及意義.方法 用免疫組化EnVision法檢測40例進行期銀屑病患者斑塊狀皮損及20例進行期銀屑病患者非皮損區皮膚、10例正常皮膚石蠟標本中的TLR4、NF-κBp65、CCL20的錶達,對其在皮損中的錶達水平進行相關性分析.結果 銀屑病皮損區角質形成細胞中TLR4、NF-κBp65、CCL20的錶達(A值分彆為0.3006±0.1200、0.4551±0.1572和0.3862±0.1304)較正常人皮膚TLR4、NF-κBp65、CCL20的錶達(A值分彆為0.1379±0.0663、0.1970±0.0679和0.1262±0.0612)及銀屑病非皮損區組(A值分彆為0.1930±0.0687、0.2626±0.0606和0.2387±0.0467)明顯上調(t值分彆為4.113、5.044、6.106、3.711、5.265和4.889,均P＜0.05).與正常人皮膚相比,銀屑病非皮損區TLR4、NF-κBp65、CCL20錶達明顯上調(t值分彆為2.118、2.685和5.608,均P＜0.05).銀屑病患者TLR4、NF-κBp65、CCL20的錶達水平間均呈正相關(r值分彆為0.766、0.851和0.885,均P＜0.05).結論 TLR4、NF-κBp65、CCL20的高錶達可能與銀屑病髮病有關.
목적 탐토Toll양수체4(TLR4)、핵인자(NF)-κBp65、CC추화인자배체20(CCL20)재은설병환자피손구급비피손구적표체급의의.방법 용면역조화EnVision법검측40례진행기은설병환자반괴상피손급20례진행기은설병환자비피손구피부、10례정상피부석사표본중적TLR4、NF-κBp65、CCL20적표체,대기재피손중적표체수평진행상관성분석.결과 은설병피손구각질형성세포중TLR4、NF-κBp65、CCL20적표체(A치분별위0.3006±0.1200、0.4551±0.1572화0.3862±0.1304)교정상인피부TLR4、NF-κBp65、CCL20적표체(A치분별위0.1379±0.0663、0.1970±0.0679화0.1262±0.0612)급은설병비피손구조(A치분별위0.1930±0.0687、0.2626±0.0606화0.2387±0.0467)명현상조(t치분별위4.113、5.044、6.106、3.711、5.265화4.889,균P＜0.05).여정상인피부상비,은설병비피손구TLR4、NF-κBp65、CCL20표체명현상조(t치분별위2.118、2.685화5.608,균P＜0.05).은설병환자TLR4、NF-κBp65、CCL20적표체수평간균정정상관(r치분별위0.766、0.851화0.885,균P＜0.05).결론 TLR4、NF-κBp65、CCL20적고표체가능여은설병발병유관.
Objective To investigate the expressions of Toll-like receptor 4 (TLR4),nuclear factorkappa B (NF-κB) p65 and chemokine CCL20 in lesional and nonlesional skin of patients with progressive plaque-type psoriasis,as well as their clinical implications.Methods Paraffin-embedded tissue samples were prepared with lesional (n =40) and nonlesional (n =20) skin from progressive plaque-type psoriatic patients as well as from the normal skin of 10 human controls.Then,the expressions of TLR4,NF-κBp65 and CCL20 were detected by immunohistochemical staining technique using the EnVision antibody complex.Differences in the expression of these parameters were assessed by t test or corrected t test,and the correlations between these proteins were evaluated by Pearson's product-moment correlation coefficient.Results The expression levels of TLR4,NF-κBp65 and CCL20 were significantly higher in lesional skin than in nonlesional skin from patients and in normal skin from normal controls (TLR4:0.3006 ± 0.1200 vs.0.1930 ± 0.0687 and 0.1379 ± 0.0663,t =5.044,4.113,respectively,both P ＜ 0.05; NF-κBp65:0.4551 ± 0.1572 vs.0.2626 ± 0.0606 and 0.1970 ± 0.0679,t =3.711,6.106,respectively,both P ＜ 0.05; CCL20:0.3862 ± 0.1304 vs.0.2387 ± 0.0467 and 0.1262 ± 0.0612,t =4.889,5.265,respectively,both P ＜ 0.05),and higher in nonlesional skin of patients than in normal skin of the controls (t =2.118,2.685,5.608,respectively,all P ＜ 0.05).Moreover,the expressions of TLR4,NF-κBp65 and CCL20 were positively correlated with each other in lesional skin of the patients (r =0.766,0.851,0.885,respectively,all P ＜ 0.05).Conclusion The up-regulated expressions of TLR4,NF-κBp65 and CCL20 might be involved in the pathogenesis of psoriasis.
