中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
9期
644-647
,共4页
徐圣经%饶彦婷%赵志国%尹雪锋%张彩萍%马朝东%张宇%陈建华%丁克云
徐聖經%饒彥婷%趙誌國%尹雪鋒%張綵萍%馬朝東%張宇%陳建華%丁剋雲
서골경%요언정%조지국%윤설봉%장채평%마조동%장우%진건화%정극운
皮肤纤维肉瘤%纤维瘤%抗原,CD99%miR-205
皮膚纖維肉瘤%纖維瘤%抗原,CD99%miR-205
피부섬유육류%섬유류%항원,CD99%miR-205
Dermatofibrosarcoma%Fibroma%Antigens,CD99%miR-205
目的 探讨隆突性皮肤纤维肉瘤(DFSP)及皮肤纤维瘤(DF)中CD99及miRNA的表达及临床意义.方法 免疫组化染色及Western印迹检测DFSP和DF中CD99蛋白表达.实时PCR检测miR-637、miR-576-5p、miR-23a、miR-23b、miR-625及miR-205在DFSP和DF中的表达差异.结果 免疫组化染色结果显示,CD99蛋白在DFSP(73.33％)中阳性表达率明显高于DF(6.77％),差异有统计学意义(P＜0.05).Western印迹检测结果显示,DFSP组织中CD99蛋白含量(1例,CD99/黏着斑蛋白=11.289)明显高于DF(2例,DF1:CD99/黏着斑蛋白=4.743,DF2:CD99/黏着斑蛋白=2.402).实时PCR检测结果显示,与DF相比,DFSP中miR-205表达明显下降(DF:DFSP=1:0.160);生物信息学分析表明,miR-205可以作用于CD99 mRNA的3'UTR区域.结论 CD99及miR-205均可作为鉴别诊断DFSP和DF的分子标记物.
目的 探討隆突性皮膚纖維肉瘤(DFSP)及皮膚纖維瘤(DF)中CD99及miRNA的錶達及臨床意義.方法 免疫組化染色及Western印跡檢測DFSP和DF中CD99蛋白錶達.實時PCR檢測miR-637、miR-576-5p、miR-23a、miR-23b、miR-625及miR-205在DFSP和DF中的錶達差異.結果 免疫組化染色結果顯示,CD99蛋白在DFSP(73.33％)中暘性錶達率明顯高于DF(6.77％),差異有統計學意義(P＜0.05).Western印跡檢測結果顯示,DFSP組織中CD99蛋白含量(1例,CD99/黏著斑蛋白=11.289)明顯高于DF(2例,DF1:CD99/黏著斑蛋白=4.743,DF2:CD99/黏著斑蛋白=2.402).實時PCR檢測結果顯示,與DF相比,DFSP中miR-205錶達明顯下降(DF:DFSP=1:0.160);生物信息學分析錶明,miR-205可以作用于CD99 mRNA的3'UTR區域.結論 CD99及miR-205均可作為鑒彆診斷DFSP和DF的分子標記物.
목적 탐토륭돌성피부섬유육류(DFSP)급피부섬유류(DF)중CD99급miRNA적표체급림상의의.방법 면역조화염색급Western인적검측DFSP화DF중CD99단백표체.실시PCR검측miR-637、miR-576-5p、miR-23a、miR-23b、miR-625급miR-205재DFSP화DF중적표체차이.결과 면역조화염색결과현시,CD99단백재DFSP(73.33％)중양성표체솔명현고우DF(6.77％),차이유통계학의의(P＜0.05).Western인적검측결과현시,DFSP조직중CD99단백함량(1례,CD99/점착반단백=11.289)명현고우DF(2례,DF1:CD99/점착반단백=4.743,DF2:CD99/점착반단백=2.402).실시PCR검측결과현시,여DF상비,DFSP중miR-205표체명현하강(DF:DFSP=1:0.160);생물신식학분석표명,miR-205가이작용우CD99 mRNA적3'UTR구역.결론 CD99급miR-205균가작위감별진단DFSP화DF적분자표기물.
Objective To detect the expressions of CD99 and miRNAs in dermatofibrosarcoma protuberan (DFSP) and dermatofibroma (DF),and to investigate their clinical implications.Methods This study included 30 DFSP and 15 DF paraffin-embedded tissue specimens collected at the Department of Pathology,Affiliated People's Hospital of Jiangsu University,from January 2002 to December 2010,as well as two DFSP and three DF fresh-frozen tissue specimens.The expression of CD99 was detected by immunohistochemistry in all of these paraffin-embedded specimens,and by Western blot in one DFSP and two DF fresh-frozen tissue specimens.Real-time PCR was carried out to determine the expression levels of miR-637,miR-576-5p,miR-23a,miR-23b,miR-625 and miR-205 in two DFSP and three DF fresh-frozen tissue specimens.Immunohistochemical results were compared by chi-square test.Results Immunohistochemistry showed that the expression rate of CD99 protein was statistically higher in DFSP than in DF paraffin-embedded tissue specimens (73.33％ vs.6.67％,P ＜ 0.05).As Western blot displayed,the CD99/vinculin ratio was higher in the fresh-frozen DFSP specimen (11.289) than in the two DF specimens (4.743 and 2.402 respectively).Real-time PCR revealed that the expression intensity of miR-205 was lower in DFSP than in DF fresh-frozen tissue specimens (0.160 vs.1).Bioinformatics analysis showed that miR-205 could target the CD99 mRNA 3'-untranslated region.Conclusion Both CD99 and miR-205 may serve as valuable markers for the differential diagnosis between DFSP and DF.
