CAJ | 학술논문

目的探讨点阵Er:YAG激光照射对紫外线所致豚鼠光老化皮肤胶原纤维和弹性纤维的影响.方法随机数字表法将60只豚鼠分为无处理组10只和模型组50只,中波紫外线(UVB)和长波紫外线(UVA)照射模型组豚鼠皮肤构建光老化模型,随机选择10只作为光老化模型组,余40只豚鼠分为2周、4周、8周、12周治疗组.点阵Er:YAG激光连续多脉冲照射治疗组豚鼠左侧背部(治疗侧)皮肤,HE染色、Masson染色、Weigert-van Gieson染色和碱水解-分光光度法比较照射后,治疗侧和对照侧皮肤组织学改变、胶原纤维、弹性纤维和羟脯氨酸含量.结果 UVA和UVB的最小红斑量4224 mJ/cm2和504 mJ/cm2隔日照射豚鼠背部皮肤,累积照射UVA 459.36 J/cm2和UVB 54.81 J/cm2,成功获得光老化模型.HE染色显示,治疗组治疗侧皮肤真皮浅层激光刺激区新生的胶原纤维和弹性纤维增加、排列致密.12周组Masson染色胶原面积比值治疗侧(0.70±0.12)较对照侧(0.63±0.08)均有增加,差异有统计学意义(f=1.18,P＜0.05)；羟脯氨酸含量治疗侧[(4.73±0.39) mg/g]较对照侧[(3.66±0.85) mg/g]亦有增加,差异有统计学意义(t=3.40,P＜ 0.05).结论点阵Er:YAG激光连续多脉冲照射可以刺激光老化豚鼠皮肤真皮浅层胶原纤维含量增加,纤细的弹性纤维新生.
목적 탐토점진Er:YAG격광조사대자외선소치돈서광노화피부효원섬유화탄성섬유적영향.방법 수궤수자표법장60지돈서분위무처리조10지화모형조50지,중파자외선(UVB)화장파자외선(UVA)조사모형조돈서피부구건광노화모형,수궤선택10지작위광노화모형조,여40지돈서분위2주、4주、8주、12주치료조.점진Er:YAG격광련속다맥충조사치료조돈서좌측배부(치료측)피부,HE염색、Masson염색、Weigert-van Gieson염색화감수해-분광광도법비교조사후,치료측화대조측피부조직학개변、효원섬유、탄성섬유화간포안산함량.결과 UVA화UVB적최소홍반량4224 mJ/cm2화504 mJ/cm2격일조사돈서배부피부,루적조사UVA 459.36 J/cm2화UVB 54.81 J/cm2,성공획득광노화모형.HE염색현시,치료조치료측피부진피천층격광자격구신생적효원섬유화탄성섬유증가、배렬치밀.12주조Masson염색효원면적비치치료측(0.70±0.12)교대조측(0.63±0.08)균유증가,차이유통계학의의(f=1.18,P＜0.05)；간포안산함량치료측[(4.73±0.39) mg/g]교대조측[(3.66±0.85) mg/g]역유증가,차이유통계학의의(t=3.40,P＜ 0.05).결론 점진Er:YAG격광련속다맥충조사가이자격광노화돈서피부진피천층효원섬유함량증가,섬세적탄성섬유신생.
Objective To evaluate the effect of fractional Er:YAG laser therapy on collagen and elastic fibers in photoaged skin of ultraviolet-irradiated Guinea pigs.Methods Sixty Guinea pigs were randomly divided into the non-intervention group (n =10) and model group (n =50) by a random number table.To establish a model of photoaging,the 50 Guinea pigs were irradiated by ultraviolet A (UVA) and ultraviolet B (UVB) on the back every other day for two months.Subsequently,the 50 mice were equally classified into five groups:photoaging group receiving no laser therapy,as well as 2-week,4-week,8-week and 12-week group receiving one,one,two and three sessions of successive multi-pulsed fractional Er:YAG laser therapy,respectively,on the left side (treatment side) of the back,with the right side as the control side.The interval of laser therapy was four weeks.Skin biopsies were obtained from the right and left side of the back of Guinea pigs after the laser therapy,and subjected to haematoxylin and eosin (HE) staining,Masson staining and Weigert-van Gieson staining.The content of hydroxyproline was measured by using an alkaline-hydrolysis method.Results The minimal erythema dose for UVA and UVB was 4224 mJ/cm2 and 504 mJ/cm2 respectively on Guinea pig skin.The model for photoaging was successfully established in 50 Guinea pigs after two months of irradiation with the cumulative dose of UVA and UVB being 459.36 J/cm2 and 54.81 J/cm2 respectively.HE staining revealed an obvious increase in newly-growing collagen and elastic fibers,which were arranged densely,in the treatment side compared with the control side of the back of Guinea pigs after laser therapy.The area ratio of collagen fibers stained positive for Masson's trichrome and content of hydroxyproline were significantly higher in the treatment side than in the control side of the 12-week group (0.70 ± 0.12 vs.0.63 ± 0.08,t =1.18,P ＜ 0.05;(4.73 ± 0.39) mg/g vs.(3.66 ± 0.85) mg/g,t =3.40,P ＜ 0.05).Conclusion Successive multi-pulsed fractional Er:YAG laser irradiation can result in an increase in collagen fibers and renewal of elastic fibers in superficial dermis of photoaged skin of Guinea pigs.