中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
11期
784-787
,共4页
银屑病%成纤维细胞生长因子1%丝裂原活化蛋白激酶1%抗原,CD34
銀屑病%成纖維細胞生長因子1%絲裂原活化蛋白激酶1%抗原,CD34
은설병%성섬유세포생장인자1%사렬원활화단백격매1%항원,CD34
Psoriasis%Fibroblast growth factor 1%Mitogen-activated protein kinase 1%Antigens,CD34
目的 探讨寻常性银屑病皮损成纤维细胞生长因子1(FGF-1)、丝裂原活化蛋白激酶1(MAPK-1)、CD34与临床病理的相关性.方法 收集30例寻常性银屑病患者的皮损,记录患者的一般情况和临床表现.用组织芯片技术和免疫组化法检测银屑病皮损中FGF-1、MAPK-1、CD34蛋白的表达,反转录PCR(RT-PCR)法检测银屑病皮损中FGF-1 mRNA的表达,统计分析患者的性别、年龄、临床分期、PASI评分、血管新生、表皮异常增生与FGF-1蛋白表达水平的相关性.结果 与对照皮肤相比,寻常性银屑病皮损中FGF-1蛋白和mRNA表达均明显上调,差异有统计学意义(P＜0.05).CD34和MAPK-1在银屑病皮损中均表达上调,且与FGF-1表达呈正相关.FGF-1表达上调与患者的性别和年龄无关,与临床分期和PASI评分相关,其中进行期FGF-1表达上调人数远多于静止期和低评分患者的人数,差异有统计学意义(P＜0.05).结论 FGF-1可能参与了寻常性银屑病表皮异常增殖和真皮乳头层血管异常增生,表达上调与疾病进展和皮损加重相关.
目的 探討尋常性銀屑病皮損成纖維細胞生長因子1(FGF-1)、絲裂原活化蛋白激酶1(MAPK-1)、CD34與臨床病理的相關性.方法 收集30例尋常性銀屑病患者的皮損,記錄患者的一般情況和臨床錶現.用組織芯片技術和免疫組化法檢測銀屑病皮損中FGF-1、MAPK-1、CD34蛋白的錶達,反轉錄PCR(RT-PCR)法檢測銀屑病皮損中FGF-1 mRNA的錶達,統計分析患者的性彆、年齡、臨床分期、PASI評分、血管新生、錶皮異常增生與FGF-1蛋白錶達水平的相關性.結果 與對照皮膚相比,尋常性銀屑病皮損中FGF-1蛋白和mRNA錶達均明顯上調,差異有統計學意義(P＜0.05).CD34和MAPK-1在銀屑病皮損中均錶達上調,且與FGF-1錶達呈正相關.FGF-1錶達上調與患者的性彆和年齡無關,與臨床分期和PASI評分相關,其中進行期FGF-1錶達上調人數遠多于靜止期和低評分患者的人數,差異有統計學意義(P＜0.05).結論 FGF-1可能參與瞭尋常性銀屑病錶皮異常增殖和真皮乳頭層血管異常增生,錶達上調與疾病進展和皮損加重相關.
목적 탐토심상성은설병피손성섬유세포생장인자1(FGF-1)、사렬원활화단백격매1(MAPK-1)、CD34여림상병리적상관성.방법 수집30례심상성은설병환자적피손,기록환자적일반정황화림상표현.용조직심편기술화면역조화법검측은설병피손중FGF-1、MAPK-1、CD34단백적표체,반전록PCR(RT-PCR)법검측은설병피손중FGF-1 mRNA적표체,통계분석환자적성별、년령、림상분기、PASI평분、혈관신생、표피이상증생여FGF-1단백표체수평적상관성.결과 여대조피부상비,심상성은설병피손중FGF-1단백화mRNA표체균명현상조,차이유통계학의의(P＜0.05).CD34화MAPK-1재은설병피손중균표체상조,차여FGF-1표체정정상관.FGF-1표체상조여환자적성별화년령무관,여림상분기화PASI평분상관,기중진행기FGF-1표체상조인수원다우정지기화저평분환자적인수,차이유통계학의의(P＜0.05).결론 FGF-1가능삼여료심상성은설병표피이상증식화진피유두층혈관이상증생,표체상조여질병진전화피손가중상관.
Objective To estimate the relationship of fibroblast growth factor-1 (FGF-1),mitogen activated protein kinase-1 (MAPK-1) and CD34 expressions with clinicopathological findings in lesions of psoriasis vulgaris.Methods Skin specimens were collected from 30 patients with psoriasis vulgaris,whose general information and clinical findings were recorded.The protein expressions of FGF-1,MAPK-1 and CD34 in these specimens were detected by tissue chip technology and immunohistochemistry,and the mRNA expression of FGF-1 by reverse transcription-PCR.The correlations of FGF-1 protein expression with patients' gender,age,clinical stage,psoriasis area and severity index (PASI) score,angiogenesis and abnormal epidermal proliferation were statistically analyzed.Results The protein and mRNA expressions of FGF-1 were significantly higher in the lesions than in the control skin (both P ＜ 0.05).The protein expressions of CD34 and MAPK-1 were significantly increased,and positively associated with the protein expression of FGF-1 in psoriatic lesions.The up-regulation of FGF-1 protein was unrelated to the age or gender of patients,but associated with clinical stage and PASI score.The proportion of patients with increased FGF-1 expression was significantly larger in patients at active stage and with high PSAI score than in those at quiescent stage and with low PSAI score (P ＜ 0.05).Conclusions In psoriasis vulgaris,FGF-1 may participate in the abnormal epidermal proliferation and vascular dysplasia in dermal papilla,and the upregulation of FGF-1 appears to be associated with the progression of disease and aggravation of lesions.
