CAJ | 학술논문

目的探讨并比较530 nm、630 nm以及850 nm发光二极管(LED)光对光老化皮肤的作用.方法选择14例皮肤光老化受试者,随机于其一侧前臂伸侧三个区域,分别给予530、630及850 nm LED光照射,每周3次,共12次.对照射部位皮肤进行皮肤镜检查,并比较照射前、照射第2、4和6周皮肤角质层含水量、经皮水分丢失量(TEWL)以及反映皮肤颜色的皮肤亮度(L*值)和皮肤黑素指数(MI值)的变化.同时,随机选择5例、5例、4例受试者在试验前和试验第6周分别取530、630及850 nm LED光照射皮肤进行组织病理检查.结果经12次630、850 nm LED光照射后,经皮肤镜检查,分别有10例、7例受试者皮肤纹理变浅,色素沉着减少；7例受试者530 nm LED光照射部位皮肤纹理加深,色素沉着增加.经630和850 nm LED光照射后,14例受试者皮肤角质层含水量均明显升高(6周时分别为37.9±7.7和34.5±7.1 au,照射前分别为33.1±6.1和32.0±7.0 au,照射前后比较,均P＜ 0.05),TEWL值均明显降低(6周时分别为9.8±2.5和10.9-2.5 9·m-2,h-1,照射前分别为14.0±1.8和14.2±2.6 9·m-2·h-1,照射前后比较,均P＜0.05),L*值与MI值均未发现明显改变(P＞0.05).530 nm组皮肤角质层含水量及TEWL值较照射前差异均无统计学意义(P＞0.05),L*值显著降低(P＜0.05),MI值明显增高(P＜0.05).组织病理检查显示,照射前真皮具有典型的光化性胶原纤维、弹性纤维变性损害,照射后3组均出现新生胶原纤维、弹性纤维,排列较照射前整齐致密.结论 630和850 nm LED光均可以改善光老化皮肤外观、屏障功能,促进胶原纤维和弹性纤维增生和重排,530 nm LED光会增加皮肤色素沉着.
목적 탐토병비교530 nm、630 nm이급850 nm발광이겁관(LED)광대광노화피부적작용.방법 선택14례피부광노화수시자,수궤우기일측전비신측삼개구역,분별급여530、630급850 nm LED광조사,매주3차,공12차.대조사부위피부진행피부경검사,병비교조사전、조사제2、4화6주피부각질층함수량、경피수분주실량(TEWL)이급반영피부안색적피부량도(L*치)화피부흑소지수(MI치)적변화.동시,수궤선택5례、5례、4례수시자재시험전화시험제6주분별취530、630급850 nm LED광조사피부진행조직병리검사.결과 경12차630、850 nm LED광조사후,경피부경검사,분별유10례、7례수시자피부문리변천,색소침착감소；7례수시자530 nm LED광조사부위피부문리가심,색소침착증가.경630화850 nm LED광조사후,14례수시자피부각질층함수량균명현승고(6주시분별위37.9±7.7화34.5±7.1 au,조사전분별위33.1±6.1화32.0±7.0 au,조사전후비교,균P＜ 0.05),TEWL치균명현강저(6주시분별위9.8±2.5화10.9-2.5 9·m-2,h-1,조사전분별위14.0±1.8화14.2±2.6 9·m-2·h-1,조사전후비교,균P＜0.05),L*치여MI치균미발현명현개변(P＞0.05).530 nm조피부각질층함수량급TEWL치교조사전차이균무통계학의의(P＞0.05),L*치현저강저(P＜0.05),MI치명현증고(P＜0.05).조직병리검사현시,조사전진피구유전형적광화성효원섬유、탄성섬유변성손해,조사후3조균출현신생효원섬유、탄성섬유,배렬교조사전정제치밀.결론 630화850 nm LED광균가이개선광노화피부외관、병장공능,촉진효원섬유화탄성섬유증생화중배,530 nm LED광회증가피부색소침착.
Objective To evaluate the efficacy of light-emitting diode (LED) irradiation at 530 nm,630 nm and 850 nm in the treatment of photoaged skin.Methods A total of 14 adults with photoaged extensor forearm were recruited in this study.Three areas were selected at the extensor forearm in each of these subjects and received LED irradiation at 530 nm,630 nm and 850 nm,respectively,thrice a week for 12 sessions.Dermoscopy was used to visualize these areas before and after the irradiation,the Corueometer CM 825 to measure stratum corneum (SC) hydration,Tewameter TM 300 to determine transepidermal water loss (TEWL),and CM-2600d spectrophotometer to measure L* value and melanin index (MI).Tissue specimens were obtained from these areas irradiated with LED at 530 nm (n =5),630 nm (n =5) and 850 nm (n =4) separately before and 6 weeks after the initial irradiation,and subjected to histopathological examination.Results Both skin texture and pigmentation were improved in 10 and 7 testees after 12 sessions of LED irradiation at 630 nm and 850 nm,respectively,but the skin texture became rougher and pigmentation was increased in 7 testees after LED irradiation at 530 nm.After LED irradiation at 630 nm and 850 nm,the testees experienced an obvious increase in SC hydration ((37.9 ± 7.7) au and (34.5 ± 7.1) au at 6 weeks after the initial irradiation vs.(33.1 ± 6.1) au and (32.0 ± 7.0) au before irradiation,both P ＜ 0.05),but a significant decrease in TEWL value ((9.8-± 2.5) and (10.9 ± 2.5) g·m-2·h-1 at 6 weeks after the initial irradiation vs.(14.0 ± 1.8) and (14.2 ± 2.6) g·m-2·h-1 before irradiation),with no significant changes in L* value or MI (both P ＞ 0.05).No evident alteration was observed in SC hydration or TEWL value (both P ＞ 0.05),while the L* value significantly decreased (P ＜ 0.05) and MI increased (P ＜ 0.05) after LED irradiation at 530 nm.Histopathological examination showed photo-induced denaturation of collagen fibers and elastic fibers in the dermis of these subjects before irradiation,as well as nascent collagen fibers and elastic fibers in a more dense and regular arrangement after irradiation at the three wavelengths.Conclusions LED irradiation at both 630 nm and 850 nm can improve the appearance and barrier function of,and stimulate the proliferation and rearrangement of collagen fibers and elastic fibers in,photoaged skin,while LED irradiation at 530 nm increases skin pigmentation.