中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2014年
3期
172-175
,共4页
红斑狼疮,系统性%CD4阳性T淋巴细胞%miR-17%miR-20a
紅斑狼瘡,繫統性%CD4暘性T淋巴細胞%miR-17%miR-20a
홍반랑창,계통성%CD4양성T림파세포%miR-17%miR-20a
Lupus erythematosus,systemic%CD4-positive T-lymphocytes%miR-17%miR-20a
目的 探讨miR-17和miR-20a在系统性红斑狼疮(SEE)患者CD4+T细胞中的表达及与疾病活动的相关性.方法 应用实时荧光定量聚合酶链反应(PCR)检测SLE患者活动组17例、缓解组13例及健康对照组18例外周血CD4+T细胞中miR-17和miR-20a的表达水平,并与SLE患者临床指标进行相关性分析.结果 SLE患者组30例miR-17的表达水平(0.24±0.08)显著高于健康对照组(0.15±0.06),两组比较,P＜ 0.05；两组miR-20a的表达水平分别为0.19±0.10和0.12±0.09,两组比较,P＜0.05.SLE活动组、缓解组与健康对照组miR-17表达水平分别为0.27±0.07、0.20±0.05、0.16±0.08,活动组高于缓解组(P＜0.05)和健康对照组(P＜0.05)；miR-20a表达水平三组分别为0.22±0.10、0.15±0.07、0.13±0.09,活动组高于缓解组(P＜0.05)和健康对照组(P＜0.05)；缓解组miR-17和miR-20a的表达水平与健康对照组比较差异均无统计学意义(P＞0.05).SLE患者组miR-17、miR-20a的表达与SLE活动指数(SLEDAI)均呈正相关(r=0.45,P＜0.05和r=0.38,P＜0.05),与抗dsDNA抗体滴度呈正相关(r=0.54,P＜0.01和r=0.46,P＜ 0.01),而与C3水平呈负相关(r=-0.43,P＜0.05和r=-0.42,P＜0.05).结论 SLE患者外周血CD4+T细胞中miR-17、miR-20a表达上调,且miR-17、miR-20a表达水平与SLE病情活动相关.
目的 探討miR-17和miR-20a在繫統性紅斑狼瘡(SEE)患者CD4+T細胞中的錶達及與疾病活動的相關性.方法 應用實時熒光定量聚閤酶鏈反應(PCR)檢測SLE患者活動組17例、緩解組13例及健康對照組18例外週血CD4+T細胞中miR-17和miR-20a的錶達水平,併與SLE患者臨床指標進行相關性分析.結果 SLE患者組30例miR-17的錶達水平(0.24±0.08)顯著高于健康對照組(0.15±0.06),兩組比較,P＜ 0.05；兩組miR-20a的錶達水平分彆為0.19±0.10和0.12±0.09,兩組比較,P＜0.05.SLE活動組、緩解組與健康對照組miR-17錶達水平分彆為0.27±0.07、0.20±0.05、0.16±0.08,活動組高于緩解組(P＜0.05)和健康對照組(P＜0.05)；miR-20a錶達水平三組分彆為0.22±0.10、0.15±0.07、0.13±0.09,活動組高于緩解組(P＜0.05)和健康對照組(P＜0.05)；緩解組miR-17和miR-20a的錶達水平與健康對照組比較差異均無統計學意義(P＞0.05).SLE患者組miR-17、miR-20a的錶達與SLE活動指數(SLEDAI)均呈正相關(r=0.45,P＜0.05和r=0.38,P＜0.05),與抗dsDNA抗體滴度呈正相關(r=0.54,P＜0.01和r=0.46,P＜ 0.01),而與C3水平呈負相關(r=-0.43,P＜0.05和r=-0.42,P＜0.05).結論 SLE患者外週血CD4+T細胞中miR-17、miR-20a錶達上調,且miR-17、miR-20a錶達水平與SLE病情活動相關.
목적 탐토miR-17화miR-20a재계통성홍반랑창(SEE)환자CD4+T세포중적표체급여질병활동적상관성.방법 응용실시형광정량취합매련반응(PCR)검측SLE환자활동조17례、완해조13례급건강대조조18예외주혈CD4+T세포중miR-17화miR-20a적표체수평,병여SLE환자림상지표진행상관성분석.결과 SLE환자조30례miR-17적표체수평(0.24±0.08)현저고우건강대조조(0.15±0.06),량조비교,P＜ 0.05；량조miR-20a적표체수평분별위0.19±0.10화0.12±0.09,량조비교,P＜0.05.SLE활동조、완해조여건강대조조miR-17표체수평분별위0.27±0.07、0.20±0.05、0.16±0.08,활동조고우완해조(P＜0.05)화건강대조조(P＜0.05)；miR-20a표체수평삼조분별위0.22±0.10、0.15±0.07、0.13±0.09,활동조고우완해조(P＜0.05)화건강대조조(P＜0.05)；완해조miR-17화miR-20a적표체수평여건강대조조비교차이균무통계학의의(P＞0.05).SLE환자조miR-17、miR-20a적표체여SLE활동지수(SLEDAI)균정정상관(r=0.45,P＜0.05화r=0.38,P＜0.05),여항dsDNA항체적도정정상관(r=0.54,P＜0.01화r=0.46,P＜ 0.01),이여C3수평정부상관(r=-0.43,P＜0.05화r=-0.42,P＜0.05).결론 SLE환자외주혈CD4+T세포중miR-17、miR-20a표체상조,차miR-17、miR-20a표체수평여SLE병정활동상관.
Objective To investigate the expressions of miR-17 and miR-20a in CD4+ T cells from patients with systemic lupus erythematosus (SLE) and their correlations with disease activity.Methods CD4+ T cells were isolated from the venous blood of 30 patients with SLE and 18 healthy human controls.RNA was extracted from the CD4+T cells,and real-time fluorescence-based quantitative PCR (qPCR) was performed to determine the expression levels of miR-17 and miR-20a.The intergroup comparison of miR-17 and miR-20a expression levels was done using t test and Mann-Whitney test,and the correlations of miR-17 and miR-20a expressions with clinical parameters were assessed using Pearson or Spearman correlation coefficients.Results The patients with SLE showed increased expression levels (2-△c~) of miR-17 and miR-20a compared with the healthy controls (0.24 ± 0.08 vs.0.15 ± 0.06 for miR-17,0.19 ± 0.10 vs.0.12 ± 0.09 for miR-20a,both P ＜ 0.05).The miR-17 and miR-20a expression levels were also significantly higher in patients with active SLE than in those with inactive SLE and the healthy controls (0.27 ± 0.07 vs.0.20 ± 0.05 and 0.16 ± 0.08 for miR-17,0.22 ± 0.10 vs.0.15-0.07 and 0.13 ± 0.09 for miR-20a,all P ＜ 0.05),but similar between the patients with inactive SLE and healthy controls (both P ＞ 0.05).The expression levels of both miR-17 and miR-20a were positively correlated with SLE disease activity index (SLEDAI)(r =0.45,0.38,respectively,both P ＜ 0.05) and anti-dsDNA antibody level (r =0.54,0.46,respectively,both P ＜0.05),but negatively correlated with serum C3 levels (r =-0.43,-0.42,respectively,both P ＜ 0.05).Condusions The expressions of miR-17 and miR-20a are increased in CD4+ T cells from patients with SLE,and correlated with the disease activity in SLE.
