中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2014年
6期
385-388
,共4页
曾宪玉%姜敏%付辰%董碧麟%吴卓璇%王玮蓁
曾憲玉%薑敏%付辰%董碧麟%吳卓璇%王瑋蓁
증헌옥%강민%부신%동벽린%오탁선%왕위진
丙酸杆菌属%红霉素%克林霉素%23S rRNA%基因,erm
丙痠桿菌屬%紅黴素%剋林黴素%23S rRNA%基因,erm
병산간균속%홍매소%극림매소%23S rRNA%기인,erm
Propionibacterium%Erythromycin%Clindamycin%23S rRNA%Genes,erm
目的 探讨武汉市耐红霉素丙酸杆菌23S rRNA有无点突变以及携带ermX基因的Tn5432转座子是否转入了丙酸杆菌.方法 从痤疮患者皮损中分离丙酸杆菌,E-test法检测分离株对红霉素和克林霉素的MIC值.PCR扩增耐药株23S rRNA、ermX、ermX(cj)、IS1249a、IS1249b并测序,并在基因库中比较.结果 19株痤疮丙酸杆菌(P.acnes)和10株卵白丙酸杆菌(P.avidum)对红霉素均表现为高度耐药(MIC均＞256 μg/ml).19株P.acnes中,16株对克林霉素高度耐药(MIC＞ 256 μg/ml),3株敏感;10株P.avidum对克林霉素高度耐药(MIC＞ 256 μg/ml).19株P.acnes耐药株中,7株在相当于E.coli 23S rRNA 2058位点发现由A→G点突变,均对红霉素和克林霉素高度耐药;4株在相当于E.coli 23SrRNA 2059位点发现由A→G点突变,其中1株对克林霉素耐药,3株敏感;另外8株P.acnes扩增ermX阳性,其序列与基因库中P.acnes ermX基因100％同源.10株P.avidum中,2株ermX扩增阳性,其序列与P.acnes ermX基因100％同源;另外8株扩增ermX(cj)得到预期片段PCR产物,与基因库中Corynebactedum jeikeium ermX(cj)序列99％同源,而与P.acnes ermX基因仅有94％的同源性.10株扩增ermX基因阳性的菌株扩增IS1249a和IS1249b均阳性,而其余菌株均阴性.结论武汉市耐红霉素丙酸杆菌分别由相当于E.coli 23S rRNA 2058、2059由A→G点突变、携带ermX的Tn5432传入以及ermX(cj)传入丙酸杆菌引起.
目的 探討武漢市耐紅黴素丙痠桿菌23S rRNA有無點突變以及攜帶ermX基因的Tn5432轉座子是否轉入瞭丙痠桿菌.方法 從痤瘡患者皮損中分離丙痠桿菌,E-test法檢測分離株對紅黴素和剋林黴素的MIC值.PCR擴增耐藥株23S rRNA、ermX、ermX(cj)、IS1249a、IS1249b併測序,併在基因庫中比較.結果 19株痤瘡丙痠桿菌(P.acnes)和10株卵白丙痠桿菌(P.avidum)對紅黴素均錶現為高度耐藥(MIC均＞256 μg/ml).19株P.acnes中,16株對剋林黴素高度耐藥(MIC＞ 256 μg/ml),3株敏感;10株P.avidum對剋林黴素高度耐藥(MIC＞ 256 μg/ml).19株P.acnes耐藥株中,7株在相噹于E.coli 23S rRNA 2058位點髮現由A→G點突變,均對紅黴素和剋林黴素高度耐藥;4株在相噹于E.coli 23SrRNA 2059位點髮現由A→G點突變,其中1株對剋林黴素耐藥,3株敏感;另外8株P.acnes擴增ermX暘性,其序列與基因庫中P.acnes ermX基因100％同源.10株P.avidum中,2株ermX擴增暘性,其序列與P.acnes ermX基因100％同源;另外8株擴增ermX(cj)得到預期片段PCR產物,與基因庫中Corynebactedum jeikeium ermX(cj)序列99％同源,而與P.acnes ermX基因僅有94％的同源性.10株擴增ermX基因暘性的菌株擴增IS1249a和IS1249b均暘性,而其餘菌株均陰性.結論武漢市耐紅黴素丙痠桿菌分彆由相噹于E.coli 23S rRNA 2058、2059由A→G點突變、攜帶ermX的Tn5432傳入以及ermX(cj)傳入丙痠桿菌引起.
목적 탐토무한시내홍매소병산간균23S rRNA유무점돌변이급휴대ermX기인적Tn5432전좌자시부전입료병산간균.방법 종좌창환자피손중분리병산간균,E-test법검측분리주대홍매소화극림매소적MIC치.PCR확증내약주23S rRNA、ermX、ermX(cj)、IS1249a、IS1249b병측서,병재기인고중비교.결과 19주좌창병산간균(P.acnes)화10주란백병산간균(P.avidum)대홍매소균표현위고도내약(MIC균＞256 μg/ml).19주P.acnes중,16주대극림매소고도내약(MIC＞ 256 μg/ml),3주민감;10주P.avidum대극림매소고도내약(MIC＞ 256 μg/ml).19주P.acnes내약주중,7주재상당우E.coli 23S rRNA 2058위점발현유A→G점돌변,균대홍매소화극림매소고도내약;4주재상당우E.coli 23SrRNA 2059위점발현유A→G점돌변,기중1주대극림매소내약,3주민감;령외8주P.acnes확증ermX양성,기서렬여기인고중P.acnes ermX기인100％동원.10주P.avidum중,2주ermX확증양성,기서렬여P.acnes ermX기인100％동원;령외8주확증ermX(cj)득도예기편단PCR산물,여기인고중Corynebactedum jeikeium ermX(cj)서렬99％동원,이여P.acnes ermX기인부유94％적동원성.10주확증ermX기인양성적균주확증IS1249a화IS1249b균양성,이기여균주균음성.결론무한시내홍매소병산간균분별유상당우E.coli 23S rRNA 2058、2059유A→G점돌변、휴대ermX적Tn5432전입이급ermX(cj)전입병산간균인기.
Objective To determine whether erythromycin-resistant propionibacteria isolated from patients with acne in Wuhan city harbor 23S rRNA gene mutations as well as the transposon Tn5432 carrying ermX genes.Methods Twenty-nine Propionibacterium strains isolated from outpatients with acne in Wuhan city were included in this study.The E-test method was used to determine the susceptibility of these strains to erythromycin and clindamycin.PCR was performed to amplify the 23S rRNA,ermX,ermX (cj),IS1249a and IS1249b genes from resistant strains followed by DNA sequencing and nucleotide alignment.Results Among the 29 Propionibacterium strains,19 were identified as P.acnes and 10 as P.avidum.All of these Propionibacterium strains were resistant to erythromycin (MIC ＞ 256 μg/ml) and clindamycin (MIC ＞ 256 μg/ml),except for 3 P.acnes strains sensitive to clindamycin.Seven P.acnes strains resistant to both antibiotics exhibited an A→G transition at a position cognate with Escherichia coli 23S rRNA 2058.An A→G transition at a position cognate with E.coli 23S rRNA 2059 was identified in one clindamycin-resitant and three clindamycin-sensitive P.acnes isolates.The ermX gene was found in the remaining 8 P.acnes isolates and 2 P.avidum isolates,with the sequence 100％ identical to the reference sequence of the ermX gene of P.acnes in Genbank.Meanwhile,the ermX (cj) gene was successfully amplified from the other 8 P.avidum isolates,which showed 99％ sequence homology with the ermX (cj) gene of Corynebacterium jeikeium,but 94％ homology with the ermX gene of P.acnes in Genbank.Both IS1249a and IS1249b genes were amplified in the 10 ermX gene-positive Propionibacterium strains,but not in the other ermX gene-negative strains.Conclusions The erythromycin resistance in Propionibacterium isolates from Wuhan city may be associated with the A→G transition at the E.coli equivalent bases 2058 and 2059 of the 23S rRNA gene,as well as the presence of the erm X (transferred through the transposon Tn5432) and ermX (c j) genes.
