CAJ | 학술논문

目的研究肝癌干细胞与肿瘤血管生成的关系及其临床意义.方法采用免疫组织化学染色检测61例原发性肝癌组织中CD90、CD133和CD34的表达水平,探索其与肝癌患者术后生存预后间的关系.将患者分为3组,1组为高危组,CD90表达和(或)CD133合并肿瘤微血管密度(microvessel density,MVD)高表达.2组为中危组,CD90表达和(或)CD133而MVD低表达或不表达CD90和CD133,但MVD高表达.3组为低危组,不表达CD90和CD133且MVD低表达.结果肝癌组织标本中CD90、CD133阳性率分别为29.5％(18/61)、31.1％(19/61),CD90和(或)CD133表达阳性率为45.9％ (28/61),有血管侵犯、肿瘤分化程度Ⅲ/Ⅳ、包膜不完整、TNM分期Ⅲ/Ⅳ患者CD90和(或)CD133表达阳性率明显高于无血管侵犯、肿瘤分化程度Ⅰ/Ⅱ、包膜完整、TNM分期Ⅰ/Ⅱ患者,CD90/CD133与MVD表达在肝癌组织中表达呈正相关(r=0.5,P＜0.05),与不表达CD90和CD133且MVD低表达相比,表达CD90和(或)CD133且MVD高表达的患者的术后总体生存率和无瘤生存率更差. 结论高表达肝癌干细胞表面标记物和肝癌血管生成有一定关系,两者结合可以预测肝癌患者术后生存状况.
목적 연구간암간세포여종류혈관생성적관계급기림상의의.방법 채용면역조직화학염색검측61례원발성간암조직중CD90、CD133화CD34적표체수평,탐색기여간암환자술후생존예후간적관계.장환자분위3조,1조위고위조,CD90표체화(혹)CD133합병종류미혈관밀도(microvessel density,MVD)고표체.2조위중위조,CD90표체화(혹)CD133이MVD저표체혹불표체CD90화CD133,단MVD고표체.3조위저위조,불표체CD90화CD133차MVD저표체.결과 간암조직표본중CD90、CD133양성솔분별위29.5％(18/61)、31.1％(19/61),CD90화(혹)CD133표체양성솔위45.9％ (28/61),유혈관침범、종류분화정도Ⅲ/Ⅳ、포막불완정、TNM분기Ⅲ/Ⅳ환자CD90화(혹)CD133표체양성솔명현고우무혈관침범、종류분화정도Ⅰ/Ⅱ、포막완정、TNM분기Ⅰ/Ⅱ환자,CD90/CD133여MVD표체재간암조직중표체정정상관(r=0.5,P＜0.05),여불표체CD90화CD133차MVD저표체상비,표체CD90화(혹)CD133차MVD고표체적환자적술후총체생존솔화무류생존솔경차. 결론 고표체간암간세포표면표기물화간암혈관생성유일정관계,량자결합가이예측간암환자술후생존상황.
Objective To investigate the expression of liver cancer stem cells(LCSCs) biomarkers and tumour angiogenesis in patients with hepatocellular carcinoma (HCC) and its clinical significance.Methods Immunohistochemistry was used to detect the expression of CD90,CD133 and CD34 in tumor tissues from 61 patients with HCC undergoing curative hepatectomy.Results The positive rates of CD90and CD133 in HCC tissues were 29.5％ (18/61)and 31.1％ (19/61),the positive rates of CD90 and/or CD133 in HCC were 45.9％ (28/61).The positive rate of LCSCs biomarkers in patients with vascular invasion,tumor differentiation Ⅲ/Ⅳ,non-complete encapsulation,and TNM stage Ⅲ/Ⅳ was significantly higher than those with non-vascular invasion,tumor differentiation Ⅰ / Ⅱ,with complete encapsulation,and TNM stage Ⅰ/Ⅱ.There were a significant positive correlation between LCSCs biomarkers expression and MVD(r =0.5,P ＜ 0.05).As compared with negative LCSCs expression and MVD low group,patients with a positive LCSCs biomarkers expression and MVD high in tumours had significantly lower overall survival (OS) and poor relapse-free survival (RFS).Conclusions High expression levels of LCSCs biomarkers are related to markers of angiogenesis microvessel density (MVD).By combining two of those we can classify paitients and anticipate the prognosis after surgery.