CAJ | 학술논문

目的研究Twist蛋白和表皮-钙黏附素在大肠癌组织中的表达及其与大肠癌侵袭、转移和预后之间的关系. 方法采用免疫组化EnVision法,检测Twist蛋白和表皮-钙黏附素(E-CD)在30例正常大肠黏膜、30例大肠腺瘤及142例大肠癌组织中的表达.统计采用x2检验、Fisher's确切概率法及Spearman等级相关分析.生存分析采用Kaplan-Meier检验及Cox回归模型,P＜0.05为差异有统计学意义.结果 E-CD蛋白在正常大肠黏膜组织中的强阳性表达率为90％,明显高于其在大肠腺瘤和大肠癌组织中的强阳性表达率(63％和42％,P=0.046,P=0.000).Twist蛋白在大肠癌组织中的阳性表达率为68％,明显高于其在正常大肠黏膜和大肠腺瘤组织中的阳性表达率(20％和30％,P=0.000,P=0.000).E-CD蛋白的表达与肿瘤的分化程度(P =0.048)、浸润深度(P=0.000)、静脉侵犯(P=0.000)、淋巴管侵犯(P =0.030)、淋巴结转移(P =0.001)以及Dukes分期(P =0.016)有关,而与患者的年龄(P =0.174)、性别(P =0.159)、肿瘤大小(P =0.628)以及病理组织学类型(P =0.153)无关.Twist蛋白表达与肿瘤的分化程度(P =0.000)、浸润深度(P=0.000)、静脉侵犯(P =0.000)、淋巴管侵犯(P=0.000)、淋巴结转移(P=0.000)、病理组织学类型(P=0.010)以及Dukes分期(P=0.000)有关,而与患者的年龄(P=0.866)、性别(P =0.254)以及肿瘤大小(P=0.972)无关.E-CD蛋白与Twist蛋白在大肠癌组织中的表达呈负相关(r=-0.530,P=0.000).E-CD阳性表达者的术后1、3和5年的生存率明显高于阴性表达者(P=0.000),Twist阴性表达者的术后1、3和5年的生存率明显高于阳性表达者(P =0.000).静脉侵犯(P =0.045)、淋巴结转移(P=0.040)、Dukes分期(P =0.000)、E-CD(P =0.003)和Twist蛋白的表达(P =0.031)与大肠癌的预后密切相关.结论大肠癌组织中E-CD蛋白表达降低和Twist蛋白表达增强的患者病期晚、预后差.
목적 연구Twist단백화표피-개점부소재대장암조직중적표체급기여대장암침습、전이화예후지간적관계. 방법 채용면역조화EnVision법,검측Twist단백화표피-개점부소(E-CD)재30례정상대장점막、30례대장선류급142례대장암조직중적표체.통계채용x2검험、Fisher's학절개솔법급Spearman등급상관분석.생존분석채용Kaplan-Meier검험급Cox회귀모형,P＜0.05위차이유통계학의의.결과 E-CD단백재정상대장점막조직중적강양성표체솔위90％,명현고우기재대장선류화대장암조직중적강양성표체솔(63％화42％,P=0.046,P=0.000).Twist단백재대장암조직중적양성표체솔위68％,명현고우기재정상대장점막화대장선류조직중적양성표체솔(20％화30％,P=0.000,P=0.000).E-CD단백적표체여종류적분화정도(P =0.048)、침윤심도(P=0.000)、정맥침범(P=0.000)、림파관침범(P =0.030)、림파결전이(P =0.001)이급Dukes분기(P =0.016)유관,이여환자적년령(P =0.174)、성별(P =0.159)、종류대소(P =0.628)이급병리조직학류형(P =0.153)무관.Twist단백표체여종류적분화정도(P =0.000)、침윤심도(P=0.000)、정맥침범(P =0.000)、림파관침범(P=0.000)、림파결전이(P=0.000)、병리조직학류형(P=0.010)이급Dukes분기(P=0.000)유관,이여환자적년령(P=0.866)、성별(P =0.254)이급종류대소(P=0.972)무관.E-CD단백여Twist단백재대장암조직중적표체정부상관(r=-0.530,P=0.000).E-CD양성표체자적술후1、3화5년적생존솔명현고우음성표체자(P=0.000),Twist음성표체자적술후1、3화5년적생존솔명현고우양성표체자(P =0.000).정맥침범(P =0.045)、림파결전이(P=0.040)、Dukes분기(P =0.000)、E-CD(P =0.003)화Twist단백적표체(P =0.031)여대장암적예후밀절상관.결론 대장암조직중E-CD단백표체강저화Twist단백표체증강적환자병기만、예후차.
Objective To study Twist,E-CD expression in colorectal cancer tissues and its relationship with colorectal cancer invasion,metastasis and prognosis.Methods Immunohistochemical staining (EnVision) was used to detect E-CD,Twist expression of normal colon mucosa in 30 cases,colorectal adenoma in 30 cases and colorectal cancer tissues in 142 cases.Chi-square、Fisher's and Spearman test were used to analyze E-CD and Twist protein expression,Kaplan-Meier survival analysis and multivariate COX regression were used to analyze prognosis of patients.Results E-CD in the normal mucosa were positively expressed in 90％ cases,which was significantly higher than that in colorectal adenomas (63％) (P =0.046) and colorectal cancer tissues (42％) (P =0.000).E-CD expression was related to tumor differentiation (P =0.048),invasion depth (P =0.000),vein (P =0.000) and lymph vessel invasions (P =0.030),lymph node metastasis (P =0.001) and Dukes' stage (P =0.016),but not related to patient's age(P =0.174),gender(P =0.159),tumor size (P =0.628) and tumor histological type (P =0.153).1,3,5 year survival rate in patients with positive E-CD expression was significantly higher than that in patients with negative expression (P =0.000).Positive expression rate of Twist in colorectal cancer tissues (68％) was significantly higher than that in normal mucosa (20％,P =0.000) and colorectal adenomas (30％,P =0.000).Twist expression was related to tumor histological type (P =0.000),differentiation(P =0.000),invasion depth(P =0.000),vein(P =0.000) and lymph vessel invasions(P =0.000),lymph node metastasis(P =0.010) and Dukes' stage(P =0.000).1,3,5 year's survival rate of Twist-negative expression patients was significantly higher than that in patients with positive expression (P =0.000).E-CD and Twist in colorectal cancer tissues were negatively correlated (r =-0.530,P =0.000).COX multivariate analysis shows that vein invasion (P =0.045),lymph node metastasis (P =0.040),Dukes' stage (P =0.000),E-CD (P =0.003) and Twist (P =0.031) were independent prognostic indicators.Conclusions E-CD and Twist expression in colorectal cancer are related to tumor invasion,metastasis and prognosis.Low E-CD expression and high Twist expression are related to poor prognosis of colorectal cancer patients.