中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2013年
1期
52-55
,共4页
陈积贤%张洁%孙晓雷%薛迪新%任约翰%余红敏%吴伟力%张仁虎%余铭
陳積賢%張潔%孫曉雷%薛迪新%任約翰%餘紅敏%吳偉力%張仁虎%餘銘
진적현%장길%손효뢰%설적신%임약한%여홍민%오위력%장인호%여명
结肠肿瘤%细胞迁移分析%肿瘤细胞,培养的
結腸腫瘤%細胞遷移分析%腫瘤細胞,培養的
결장종류%세포천이분석%종류세포,배양적
Colonic neoplasms%Cell migration assays%Tumor cells,cultured
目的 探讨抑制赖氨酸特异性脱甲基酶1(lysine specific demet hylase 1,LSDl)活性对人结肠癌细胞迁移的影响.方法 本研究应用反苯环丙胺抑制LSD1酶的活性,通过划痕试验和Transwall实验在体外观察人结肠癌Lovo细胞迁移和侵袭的变化.用RT-PCR和Western blot法检测LSD1的表达和H3K4me2甲基化水平. 结果 经过不同剂量反苯环丙胺(40、60和80 μmol/L)处理后,细胞增殖实验结果显示对人结肠癌Lovo细胞增殖无影响(P＞0.05)；60μmol/L反苯环丙胺干预后,划痕试验结果发现Lovo细胞迁移明显受抑制.TranswaⅡ实验发现穿过小室的细胞数:60 μmol/L反苯环丙胺组为(69.7±2.5),而对照组为(222.7±11.0),两者比较差异有统计学意义(P＜0.05).RT-PCR检测显示Lovo细胞中的LSDl mRNA和蛋白水平的表达无改变,但H3 K4me2甲基化水平升高.结论 60μmol/L反苯环丙胺能抑制结肠癌Lovo细胞的LSD1酶活性,降低细胞的迁移和侵袭能力.
目的 探討抑製賴氨痠特異性脫甲基酶1(lysine specific demet hylase 1,LSDl)活性對人結腸癌細胞遷移的影響.方法 本研究應用反苯環丙胺抑製LSD1酶的活性,通過劃痕試驗和Transwall實驗在體外觀察人結腸癌Lovo細胞遷移和侵襲的變化.用RT-PCR和Western blot法檢測LSD1的錶達和H3K4me2甲基化水平. 結果 經過不同劑量反苯環丙胺(40、60和80 μmol/L)處理後,細胞增殖實驗結果顯示對人結腸癌Lovo細胞增殖無影響(P＞0.05)；60μmol/L反苯環丙胺榦預後,劃痕試驗結果髮現Lovo細胞遷移明顯受抑製.TranswaⅡ實驗髮現穿過小室的細胞數:60 μmol/L反苯環丙胺組為(69.7±2.5),而對照組為(222.7±11.0),兩者比較差異有統計學意義(P＜0.05).RT-PCR檢測顯示Lovo細胞中的LSDl mRNA和蛋白水平的錶達無改變,但H3 K4me2甲基化水平升高.結論 60μmol/L反苯環丙胺能抑製結腸癌Lovo細胞的LSD1酶活性,降低細胞的遷移和侵襲能力.
목적 탐토억제뢰안산특이성탈갑기매1(lysine specific demet hylase 1,LSDl)활성대인결장암세포천이적영향.방법 본연구응용반분배병알억제LSD1매적활성,통과화흔시험화Transwall실험재체외관찰인결장암Lovo세포천이화침습적변화.용RT-PCR화Western blot법검측LSD1적표체화H3K4me2갑기화수평. 결과 경과불동제량반분배병알(40、60화80 μmol/L)처리후,세포증식실험결과현시대인결장암Lovo세포증식무영향(P＞0.05)；60μmol/L반분배병알간예후,화흔시험결과발현Lovo세포천이명현수억제.TranswaⅡ실험발현천과소실적세포수:60 μmol/L반분배병알조위(69.7±2.5),이대조조위(222.7±11.0),량자비교차이유통계학의의(P＜0.05).RT-PCR검측현시Lovo세포중적LSDl mRNA화단백수평적표체무개변,단H3 K4me2갑기화수평승고.결론 60μmol/L반분배병알능억제결장암Lovo세포적LSD1매활성,강저세포적천이화침습능력.
Objective To investigate the effect of LSD1 on the invasion and migration of human colon cancer cells.Methods MAO inhibitor and gene silence was used to observe the effect of LSD1 on the invasion and migration of human colon cancer Lovo cells by scratch test and Transwell test in vitro.The gene expression of LSD1 was analyzed by RT-PCR and globle histone methylation in Lovo cells was detected by Western blot.Results At concentrations of 40,60 and 80 μmol/L MAO inhibitor Tranylcypromine (TCP),have no effect on the cell growth of human colon cancer Lovo cells (P ＞ 0.05).The scratch test showed that of TCP at 60 μmol/L effectively inhibited the migration of Lovo cells.The cell numbers of migration through the well were (69.7 ± 2.5) (60 μmol/L TCP group) and (222.7 ± 11.0) (control group) (P ＜0.05),while 60 μmol/L TCP didn't affect the expression of mRNA and protein level of LSD1 but increased the expression of H3K4me2 in Lovo cells.Conclusions MAO inhibitor TCP can inhibit the invasion and migration of human colon cancer Lovo cells at a concentration of 60 μmol/L.
