CAJ | 학술논문

目的观察莪术醇对胃癌细胞株BGC823细胞凋亡的影响,并探讨其作用机制.方法体外培养BGC823细胞,分别加入12.5、25、50、100 mg/L莪术醇培养液,对照组加入10 ml/L无水乙醇培养液,分别孵育24 h和48 h,采用MTT法分析增殖率；应用流式细胞仪检测各浓度莪术醇处理BGC823细胞48 h的凋亡率及细胞周期的分布；分光光度法检测Caspase-3活性；100 mg/L莪术醇孵育BGC823细胞48 h后,用RT-PCR和Western blot法检测Caspase-3、Bcl-2、Bax和Survivin mRNA和蛋白的表达水平.结果莪术醇抑制BGC823细胞增殖,并且随浓度的增加和时间的延长而加强；不同浓度莪术醇处理BGC823细胞,均使处于G0/G1期的细胞显著增加,S期细胞明显减少(P＜0.05)；细胞凋亡率随莪术醇浓度增加而升高(P＜0.05)；Caspase-3活性呈浓度依赖性增加(P＜0.05)；100 mg/L莪术醇孵育BGC823细胞48 h后,Caspase-3、Bax表达均显著升高(P＜0.05),Survivin、Bcl-2表达均显著下降(P＜0.05),Bcl-2/Bax比值显著降低(P＜0.05).结论莪术醇对胃癌BGC823细胞生长具有抑制作用,阻滞细胞周期于G0/G1期,并促进其凋亡.其作用与增强Caspase-3的活性,上调Caspase-3、Bax表达,下调Survivin、Bcl-2表达及降低Bcl-2/Bax比值有关.
목적 관찰아술순대위암세포주BGC823세포조망적영향,병탐토기작용궤제.방법 체외배양BGC823세포,분별가입12.5、25、50、100 mg/L아술순배양액,대조조가입10 ml/L무수을순배양액,분별부육24 h화48 h,채용MTT법분석증식솔；응용류식세포의검측각농도아술순처리BGC823세포48 h적조망솔급세포주기적분포；분광광도법검측Caspase-3활성；100 mg/L아술순부육BGC823세포48 h후,용RT-PCR화Western blot법검측Caspase-3、Bcl-2、Bax화Survivin mRNA화단백적표체수평.결과 아술순억제BGC823세포증식,병차수농도적증가화시간적연장이가강；불동농도아술순처리BGC823세포,균사처우G0/G1기적세포현저증가,S기세포명현감소(P＜0.05)；세포조망솔수아술순농도증가이승고(P＜0.05)；Caspase-3활성정농도의뢰성증가(P＜0.05)；100 mg/L아술순부육BGC823세포48 h후,Caspase-3、Bax표체균현저승고(P＜0.05),Survivin、Bcl-2표체균현저하강(P＜0.05),Bcl-2/Bax비치현저강저(P＜0.05).결론 아술순대위암BGC823세포생장구유억제작용,조체세포주기우G0/G1기,병촉진기조망.기작용여증강Caspase-3적활성,상조Caspase-3、Bax표체,하조Survivin、Bcl-2표체급강저Bcl-2/Bax비치유관.
Objective To investigate the effect of curcumol on apoptosis of human gastric carcinoma cell line BGC823 and the molecular nechanisms.Methods BGC823 cells were cultured and treated with different curcumol concentration (12.5,25,50 and 100 mg/L) for 24 h and 48 h,and the growth inhibition were tested by thiazolyl blue terazolium bromide (MTF) assay.Flow cytometry (FCM) were used to measure the cell apoptosis rate and cell cycle of BGC823 cells.Caspase-3 activity was assessed by colorimetric assay.Cells treated with 100 mg/L curcunol for 48 h were collected and subjected to RTPCR and Western blot assays for the expression of Caspase-3,Bcl-2,Bax and Survivin.Results There was a time-and dose-dependent inhibition of cell proliferation of BGC823 cells by curcumol.Tbe cells in G0/G1 phase increased,and in S phase decreased on exposure to curcumol for 24 h.FCM analysis also indicated that the apoptosis rate of BGC823 cells increased in dose-dependent manner (P ＜ 0.05).Curcumol increased the activity of Caspase-3 dose-dependently (P ＜ 0.05).RT-PCR and Western blot indicated that curcumol decreased Bcl-2 and Survivin expression as well as increased Caspase-3 and Bax expression (P ＜ 0.05).Conclusions Curcumol inhibits BGC823 cell growth,arresting cells in G0/G1 phase and inducing cell apoptosis.The mechanism may be related with increasing the activity of Caspase-3,down-regulating the expression of Bcl-2 and Survivin,and up-regulating the expression of Caspase-3 and Bax.