CAJ | 학술논문

目的探讨MACC1和C-MET蛋白在肝细胞癌组织中的表达及意义.方法采用免疫组化和Western blot检测51例肝细胞癌组织、相应癌旁组织和13例正常肝脏组织中MACC1和C-MET蛋白的表达情况,同时对患者进行随访,并分析患者生存率与临床病理资料间的关系.结果 MACC1和C-MET蛋白在肝细胞癌组织中总的阳性表达率分别为80.4％和76.5％,相对表达量分别为0.645±0.047和0.504±0.023,均明显高于相应癌旁组织及正常肝脏组织(分别F=173.308,252.817,均P=0.000),且两者表达呈正相关(r=0.870,P=0.001);MACC1和C-MET蛋白表达阳性患者的3年生存率明显低于阴性患者(分别x2 =3.934,4.439,均P＜0.05),两者的阳性率及表达量均与肝细胞癌的TNM分期、门脉癌栓、病理分型及包膜侵犯显著相关(P＜0.05). 结论 MACC1和C-MET的异常表达与肝细胞癌的恶性进展相关,MACC1有作为进展期肝癌预后的独立分子指标和肝癌治疗新靶点的潜在价值.
목적 탐토MACC1화C-MET단백재간세포암조직중적표체급의의.방법 채용면역조화화Western blot검측51례간세포암조직、상응암방조직화13례정상간장조직중MACC1화C-MET단백적표체정황,동시대환자진행수방,병분석환자생존솔여림상병리자료간적관계.결과 MACC1화C-MET단백재간세포암조직중총적양성표체솔분별위80.4％화76.5％,상대표체량분별위0.645±0.047화0.504±0.023,균명현고우상응암방조직급정상간장조직(분별F=173.308,252.817,균P=0.000),차량자표체정정상관(r=0.870,P=0.001);MACC1화C-MET단백표체양성환자적3년생존솔명현저우음성환자(분별x2 =3.934,4.439,균P＜0.05),량자적양성솔급표체량균여간세포암적TNM분기、문맥암전、병리분형급포막침범현저상관(P＜0.05). 결론 MACC1화C-MET적이상표체여간세포암적악성진전상관,MACC1유작위진전기간암예후적독립분자지표화간암치료신파점적잠재개치.
Objective To explore the expression and clinical relevance of metastasis-associated colon cancer-1 (MACC1) and C-MET proteins in hepatocellular carcinoma (HCC) tissue.Methods The expressions of MACC1 and C-MET were detected in 51 specimens of HCC and paraneoplastic liver tissue,normal liver tissue in 13 healthy cases using immunohistochemistry and Western blotting.The correlations of the expressions of MACC1 and C-MET proteins were evaluated,survival rates were observed,the relationship between the expression of MACC1,C-MET proteins and the clinicopathologic features of HCC were analyzed.Results The positive rate of MACC1 and C-MET proteins was 80.4％ and 76.5％ in HCC tissue,the relative expressions were 0.645 ± 0.047 and 0.504 ± 0.023 respectively,which was significantly different from those in paraneoplastic liver tissue and normal liver tissue (respectively F =173.308,252.817,all P =0.000).The survival analysis showed that the three-year survival rate in patients with positive MACC1 and C-MET expressions was significantly lower than that in patients with negative expressions (respectively x2 =3.934,4.439,all P ＜ 0.05),the positive rate and relative expressions of MACC1 and C-MET were significantly correlated with TNM stage,portal vein cancer thrombus and pathology typing (P ＜ 0.05).Conclusions The expression of MACC1 and C-MET is associated with the malignant progression of HCC.MACC1 may serve as a independent prognostic factor for advanced HCC and a possible therapy target for the treatment of HCC.