中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2013年
7期
538-541
,共4页
白霞%张连峰%周琳%赵阿红%孙玉岭
白霞%張連峰%週琳%趙阿紅%孫玉嶺
백하%장련봉%주림%조아홍%손옥령
胰腺肿瘤%RNA,小分子干扰%Transgelin
胰腺腫瘤%RNA,小分子榦擾%Transgelin
이선종류%RNA,소분자간우%Transgelin
Pancreatic neoplasms%RNA,small interfering%Transgelin
目的 探讨靶向干扰骨架蛋白Transgelin对人胰腺癌裸鼠移植瘤生长的影响.方法 设计并体外合成靶向Transgelin的短发夹RNA(shRNA)真核表达质粒,将其转染人胰腺癌细胞BxPC3,采用RT-PCR、Western印迹法检测转染前后Transgelin表达的变化.成功造模胰腺癌裸鼠24只,按皮下注入细胞不同均分为3组:实验组(接种BxPC3/shRNA)、阴性对照组(接种空质粒转染细胞BxPC3/Neo)和空白对照组(接种未转染细胞BxPC3).每周测量肿瘤大小,术后第28天处死所有裸鼠,计算瘤体体积,治疗前、后裸鼠体重的变化以及抑瘤率.免疫组织化学法检测移植瘤组织切片中Transgelin和增殖细胞核抗原(PCNA)蛋白的表达.结果 实验组、阴性对照组和空白对照组在各时间段的肿瘤体积增长差异有统计学意义(均P ＜0.05).实验组在第21,28天肿瘤体积显著小于阴性对照组和空白对照组(均P＜ 0.05).实验组第28天瘤重为(0.74±0.21)g,阴性对照组为(1.42±0.28)g,空白组为(1.59±0.24)g.实验组瘤重与阴性、空白对照组相比差异均有统计学意义(均P ＜0.05),抑瘤率达53.5％.实验组PCNA指数显著低于阴性对照组和空白对照组(均P＜0.05).结论 靶向封闭Transgelin基因能明显抑制胰腺癌细胞BxPC3在裸鼠体内的增殖及肿瘤生长.
目的 探討靶嚮榦擾骨架蛋白Transgelin對人胰腺癌裸鼠移植瘤生長的影響.方法 設計併體外閤成靶嚮Transgelin的短髮夾RNA(shRNA)真覈錶達質粒,將其轉染人胰腺癌細胞BxPC3,採用RT-PCR、Western印跡法檢測轉染前後Transgelin錶達的變化.成功造模胰腺癌裸鼠24隻,按皮下註入細胞不同均分為3組:實驗組(接種BxPC3/shRNA)、陰性對照組(接種空質粒轉染細胞BxPC3/Neo)和空白對照組(接種未轉染細胞BxPC3).每週測量腫瘤大小,術後第28天處死所有裸鼠,計算瘤體體積,治療前、後裸鼠體重的變化以及抑瘤率.免疫組織化學法檢測移植瘤組織切片中Transgelin和增殖細胞覈抗原(PCNA)蛋白的錶達.結果 實驗組、陰性對照組和空白對照組在各時間段的腫瘤體積增長差異有統計學意義(均P ＜0.05).實驗組在第21,28天腫瘤體積顯著小于陰性對照組和空白對照組(均P＜ 0.05).實驗組第28天瘤重為(0.74±0.21)g,陰性對照組為(1.42±0.28)g,空白組為(1.59±0.24)g.實驗組瘤重與陰性、空白對照組相比差異均有統計學意義(均P ＜0.05),抑瘤率達53.5％.實驗組PCNA指數顯著低于陰性對照組和空白對照組(均P＜0.05).結論 靶嚮封閉Transgelin基因能明顯抑製胰腺癌細胞BxPC3在裸鼠體內的增殖及腫瘤生長.
목적 탐토파향간우골가단백Transgelin대인이선암라서이식류생장적영향.방법 설계병체외합성파향Transgelin적단발협RNA(shRNA)진핵표체질립,장기전염인이선암세포BxPC3,채용RT-PCR、Western인적법검측전염전후Transgelin표체적변화.성공조모이선암라서24지,안피하주입세포불동균분위3조:실험조(접충BxPC3/shRNA)、음성대조조(접충공질립전염세포BxPC3/Neo)화공백대조조(접충미전염세포BxPC3).매주측량종류대소,술후제28천처사소유라서,계산류체체적,치료전、후라서체중적변화이급억류솔.면역조직화학법검측이식류조직절편중Transgelin화증식세포핵항원(PCNA)단백적표체.결과 실험조、음성대조조화공백대조조재각시간단적종류체적증장차이유통계학의의(균P ＜0.05).실험조재제21,28천종류체적현저소우음성대조조화공백대조조(균P＜ 0.05).실험조제28천류중위(0.74±0.21)g,음성대조조위(1.42±0.28)g,공백조위(1.59±0.24)g.실험조류중여음성、공백대조조상비차이균유통계학의의(균P ＜0.05),억류솔체53.5％.실험조PCNA지수현저저우음성대조조화공백대조조(균P＜0.05).결론 파향봉폐Transgelin기인능명현억제이선암세포BxPC3재라서체내적증식급종류생장.
Objective To investigate the in vivo effect of silenced actin-associated protein Transgelin on the growth of human pancreatic carcinoma xenograft in nude mice.Methods Human pancreatic cancer cell line BxPC3 were transfected with small hairpin RNA (shRNA) eukaryotic expression vector targeting Transgelin gene.RT-PCR and Western blot were used to analyze Transgelin expression after transfection.24 animal models were randomly divided into three groups with 8 in each:Experimental group (transplanted BxPC3/Transgelin shRNA),negative control group (transplanted BxPC3/Neo) and untreated group (transplanted BxPC3).Tumor size was measured weekly.All mice were sacrificed after 28 days.Tumor volume was calculated,inhibitory effect was analyzed.Immunohistochemical staining of paraffin sections for Transgelin and proliferating cell nuclear antigen (PCNA) proteins were performed.Results Tumors varied in sizes among 3 groups (all P ＜ 0.05).On day 21 and 28 tumor was significantly smaller in experimental group than those in control groups.Tumor weighed(0.74 ±0.21) g in experimental group,lower than that in negative control group(1.42 ± 0.28) g and untreated group(1.59 ± 0.24) g (all P ＜ 0.05).The inhibitory effect was 53.5％ in experimental group.The PCNA index was significantly lower in experimental group than those in control groups (all P ＜ 0.05).Conclusions Deletion of Transgelin gene can significantly inhibit the proliferation and tumor growth of BxPC3 cells in nude mice.
