CAJ | 학술논문

目的探讨caveolin-1对兔颈总动脉吻合口狭窄的影响及其与肿瘤坏死因子-α(tumor necrosis factor,TNF-α)的关系.方法 40只新西兰兔,随机分为正常组、手术组、空转染组和转染组,每组10只.行颈总动脉血管端-端吻合,并局部转染caveolin-1质粒(转染组)或空质粒(空转染组).于术后第7天每组随机取5只新西兰兔的颈总动脉标本,用Western blot法检测caveolin-1和TNF-α的表达；其余每组的新西兰兔于术后4周处死取颈总动脉标本用于HE染色,通过Image-Pro Plus 6.0软件测量内膜与中膜面积的比值,观察颈总动脉内膜增殖情况.结果与正常组比较,手术组内膜明显增殖,内膜/中膜面积比值明显增高(P＜0.05)；与手术组比较,caveolin-1转染组内膜增殖不明显,内膜/中膜面积比值未见明显增高(P＜0.05).Western blot结果显示:转染组caveolin-1的表达明显高于其他组(P＜0.05)；而与正常组比较,手术组TNF-α表达明显升高(t=41.28,P＜0.05),而与手术组比较,转染组TNF-α表达明显下降,差异有统计学意义(t=36.37,P＜0.05).结论 Caveolin-1可抑制血管吻合口狭窄,其作用可能与下调TNF-α表达有关.
목적 탐토caveolin-1대토경총동맥문합구협착적영향급기여종류배사인자-α(tumor necrosis factor,TNF-α)적관계.방법 40지신서란토,수궤분위정상조、수술조、공전염조화전염조,매조10지.행경총동맥혈관단-단문합,병국부전염caveolin-1질립(전염조)혹공질립(공전염조).우술후제7천매조수궤취5지신서란토적경총동맥표본,용Western blot법검측caveolin-1화TNF-α적표체；기여매조적신서란토우술후4주처사취경총동맥표본용우HE염색,통과Image-Pro Plus 6.0연건측량내막여중막면적적비치,관찰경총동맥내막증식정황.결과 여정상조비교,수술조내막명현증식,내막/중막면적비치명현증고(P＜0.05)；여수술조비교,caveolin-1전염조내막증식불명현,내막/중막면적비치미견명현증고(P＜0.05).Western blot결과현시:전염조caveolin-1적표체명현고우기타조(P＜0.05)；이여정상조비교,수술조TNF-α표체명현승고(t=41.28,P＜0.05),이여수술조비교,전염조TNF-α표체명현하강,차이유통계학의의(t=36.37,P＜0.05).결론 Caveolin-1가억제혈관문합구협착,기작용가능여하조TNF-α표체유관.
Objective To investigate the effect of caveolin-1 on rabbit carotid artery anastomotic stenosis and its relationship with TNF-α.Methods 40 New Zealand white rabbits were randomly divided into normal group,surgical group,empty vector group and caveolin-1 transfecting group.Carotid artery end-to-end anastomosis was done in the rabbits except these in normal group.Five specimens were randomly taken on day 7 after surgery for Westem blot to detect the expression of caveolin-1 and TNF-α; The rest specimens were taken for HE staining at fourth week.The ratio of intima/media area were measured by Image-Pro Plus 6.0 software in order to observe the proliferation of intima.Results Compared with normal group,in surgical group intimal proliferation was significant,the intima/media ratio was significantly higher (P ＜ 0.05) ; Compared with surgical group,in caveolin-1 transfected group neointimal proliferation was not obvious,the intima/media ratio decreased (P ＜ 0.05).Western blot showed that:compared with the surgical group,caveolin-1 expression in transfected group was significantly higher (P ＜ 0.05) ; compared with normal group,the TNF-α expression in surgical group increased (t:41.28,P ＜ 0.05) ; Compared with surgical group,TNF-α expression in transfected group decreased significantly (t:36.37,P ＜ 0.05).Conclusions Caveolin-1 inhibits vascular anastomotic stenosis,possibly by down-regulating TNF-α expression.