CAJ | 학술논문

目的探讨G蛋白偶联受体34(G-protein coupled receptor 34,GPR34)的表达与肝细胞癌发生发展的相关性.方法应用肝细胞癌组织芯片和免疫组织化学技术,检测GPR34蛋白在44例肝细胞癌组织中的表达,X2检验和Fisher检验及Spearman相关检验,分析GPR34表达与肝细胞癌临床病理特点的相关性,应用Kaplan-Meier方法计算肝细胞癌患者术后5年生存率及生存时间.结果 GPR34在肝细胞癌组织中的表达比癌旁组织明显上调(P=0.003);GPR34表达与肝细胞癌大小(P =0.024)、小肝癌(P=0.030)和浸润深度(p=0.012)显著相关,与性别、年龄、肝硬化、组织分化程度及临床分期无明显相关性(P＞0.05);高表达和低表达GPR34的患者术后5年累积生存率分别为56.6％和66.0％,中位生存时间为59.0(95％ CI:49.9～68.1)个月和65.0(95％CI:56.4～73.6)个月,Kaplan-Meier分析显示其差异有统计学意义(x2=5.617,P=0.018).多因素分析显示,GPR34可以作为一个独立的预后评估因素(P=0.037).结论 GPR34在肝细胞癌组织中的表达上调,提示GPR34在其中具有促癌作用.GPR34表达上调与肝细胞癌大小、小肝癌、浸润深度和患者的生存时间相关.GPR34表达上调可以作为肝细胞癌预后不良评估的一个潜在影响因素.
목적 탐토G단백우련수체34(G-protein coupled receptor 34,GPR34)적표체여간세포암발생발전적상관성.방법 응용간세포암조직심편화면역조직화학기술,검측GPR34단백재44례간세포암조직중적표체,X2검험화Fisher검험급Spearman상관검험,분석GPR34표체여간세포암림상병리특점적상관성,응용Kaplan-Meier방법계산간세포암환자술후5년생존솔급생존시간.결과 GPR34재간세포암조직중적표체비암방조직명현상조(P=0.003);GPR34표체여간세포암대소(P =0.024)、소간암(P=0.030)화침윤심도(p=0.012)현저상관,여성별、년령、간경화、조직분화정도급림상분기무명현상관성(P＞0.05);고표체화저표체GPR34적환자술후5년루적생존솔분별위56.6％화66.0％,중위생존시간위59.0(95％ CI:49.9～68.1)개월화65.0(95％CI:56.4～73.6)개월,Kaplan-Meier분석현시기차이유통계학의의(x2=5.617,P=0.018).다인소분석현시,GPR34가이작위일개독립적예후평고인소(P=0.037).결론 GPR34재간세포암조직중적표체상조,제시GPR34재기중구유촉암작용.GPR34표체상조여간세포암대소、소간암、침윤심도화환자적생존시간상관.GPR34표체상조가이작위간세포암예후불량평고적일개잠재영향인소.
Objective To determine the expression of G-protein coupled receptor 34 (GPR34) in hepatocellular carcinoma tissues.Methods Immunohistochemistry and tissue-array were used to determine GPR34 expression in human hepatocellular carcinoma tissues and matched adjacent tissues.A statistical analysis was performed to establish the potential correlation between GPR34 expression and the patients'clinicopathological characteristics,tumor progression,and prognosis.Results GPR34 is up-regulated in primary hepatocellular carcinoma tissues compared with matched adjacent tissues (P =0.003),and it was shown that GPR34 expression is significantly correlated with tumor size (P =0.024),small hepatocellular carcinoma(0.030) and infiltration(P =0.012),but not with sex,age,grade,chnical stage (all P ＞0.05) ; it was also shown that GPR34 expression had a significant influence on prognosis (X2 =5.617,P=0.018).Multivariate analyses showed that high GPR34 expression is an independent poor prognostic factor for overall survival (P =0.037).Conclusions The up-regulation of GPR34 acts as an potential prooncogene in the development and progression of hepatocellular carcinoma.GPR34 may be a useful diagnostic or prognostic molecular biomarker,and a potential target for therapeutic intervention.