CAJ | 학술논문

目的研究细胞窖蛋白-1(caveolin-1,Cav-1)在胰腺癌上皮间质转化(epithelialmesenchymal transition,EMT)和转移中的作用及其相关机制.方法免疫荧光技术、Western blot检测Cav-1及EMT分子标志物E-cadherin,β-catenin,vimentin,N-cadherin在高转移胰腺癌细胞系(L3.7,Panc02-H7)及低转移胰腺癌细胞系(COLO357,Panc02)中的表达,倒置相差显微镜观察上述胰腺癌细胞形态.Cav-1及相应的空载体质粒转染COLO357细胞,检测Cav-1及EMT分子标志物的表达,并观察细胞形态变化.结果高转移胰腺癌细胞(L3.7,Panc02-H7)Cav-1及间质细胞标志物高表达,上皮细胞标志物低表达,而低转移胰腺癌细胞(COLO357,Panc02)Cav-1及上皮细胞标志物高表达,间质细胞标志物低表达.Cav-1质粒转染COLO357细胞后,Cav-1表达上调,E-cadherin表达降低而vimentin表达升高,且COLO357细胞由上皮细胞形态向间质细胞形态转变.结论胰腺癌细胞中Cav-1表达升高与胰腺癌EMT及转移密切相关.
목적 연구세포교단백-1(caveolin-1,Cav-1)재이선암상피간질전화(epithelialmesenchymal transition,EMT)화전이중적작용급기상관궤제.방법 면역형광기술、Western blot검측Cav-1급EMT분자표지물E-cadherin,β-catenin,vimentin,N-cadherin재고전이이선암세포계(L3.7,Panc02-H7)급저전이이선암세포계(COLO357,Panc02)중적표체,도치상차현미경관찰상술이선암세포형태.Cav-1급상응적공재체질립전염COLO357세포,검측Cav-1급EMT분자표지물적표체,병관찰세포형태변화.결과 고전이이선암세포(L3.7,Panc02-H7)Cav-1급간질세포표지물고표체,상피세포표지물저표체,이저전이이선암세포(COLO357,Panc02)Cav-1급상피세포표지물고표체,간질세포표지물저표체.Cav-1질립전염COLO357세포후,Cav-1표체상조,E-cadherin표체강저이vimentin표체승고,차COLO357세포유상피세포형태향간질세포형태전변.결론 이선암세포중Cav-1표체승고여이선암EMT급전이밀절상관.
Objective To study the relationship between the expression of Caveolin-1 (Cav-1) and epithelial-mesenchymal transition(EMT) or metastasis in human pancreatic cancer.Methods The Cav-1 protein and EMT markers in highly metastatic pancreatic cancer cell lines (L3.7,Panc02-H7) and poorly metastatic pancreatic cancer cell lines (COLO357,Panc02) was detected by Western blotting and immunofluorescence.The morphology of the pancreatic cancer cells were observed under phase-contrast photomicrographs.The plasmids pcDNA3.1-caveolin-1 and control vector pcDNA3.1 were transfected into COLO357 cells by Lipofectamine LTX.The expression of Cav-1 protein,E-cadherin and vimentin were detected,and the morphology of COLO357 cells observed.Results L3.7 and Panc02-H7 cells had strong positive Cav-1 staining and high expression of mesenchymal marker (vimentin,N-cadherin) and low epithelial marker (E-cadherin,β-catenin),whereas COLO357 and Panc02 cells with typical epithelial morphology were weak positive in Cav-1 staining and of low expression of vimentin,N-cadherin and high expression of E-cadherin,β-catenin.In Cav-1 transfected COLO357 cells vimentin expression increased,and E-cadherins decreased resulting in typical morphology changes of EMT.Conclusions Overexpression of caveolin-1 is correlated with epithelial-mesenchymal transition of pancreatic cancer cells and cancer metastasis.