中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2014年
10期
763-766
,共4页
胃肿瘤%增殖细胞核抗原%受体,人表皮生长因子%预后
胃腫瘤%增殖細胞覈抗原%受體,人錶皮生長因子%預後
위종류%증식세포핵항원%수체,인표피생장인자%예후
Stomach neoplasms%Proliferating cell nuclear antigen%Receptor,epidermal growth factor%Prognosis
目的 研究胃癌组织中泛素偶联酶UbcH 10的表达与胃癌病理分级、临床分期及预后之间的关系.方法 采用免疫组织化学方法检测有完整随访资料的117例胃癌组织中UbcH10、Ki-67及人表皮生长因子受体2的表达.用Real-time PCR和Western blot检测43例胃癌新鲜标本中UbcH10 mRNA和蛋白的含量.结果 本组117例胃癌组织中UbcH10蛋白的阳性率为83％(97/117),癌旁正常胃黏膜组织中阳性率为55％(64/117),两者相比差异具有统计学意义(Rs=-0.397,P＜0.01).胃癌组织中UbcH 10蛋白的表达与患者的性别、年龄、肿瘤部位、肿瘤直径无关(Rs =0.001、-0.084、0.052、-0.156,均P＞0.05),但与肿瘤分化、浸润深度、淋巴结转移及TNM分期相关(Rs =0.369、-0.378、-0.276、0.421,均P＜0.05).UbcH10与增殖抗原Ki-67、人表皮生长因子受体2表达呈正相关(Rs =0.834、0.281,均P＜0.01).胃癌组织中UbcH10 mRNA的表达量高于癌旁正常组织(△Ct=32.2±5.19、△Ct=4.69±1.26,t=26.8,P＜0.01),UbcH10蛋白在胃癌组织中的表达水平高于癌旁正常组织(0.74±0.45、0.11±0.04,t=8.09,P＜0.01).UbcH10阳性表达胃癌患者5年生存率低于UbcH10阴性表达患者(x2=13.116,P＜0.01).结论 UbcH10在胃癌组织中表达增强,UbcH10的表达可能与胃癌患者的预后有关.
目的 研究胃癌組織中汎素偶聯酶UbcH 10的錶達與胃癌病理分級、臨床分期及預後之間的關繫.方法 採用免疫組織化學方法檢測有完整隨訪資料的117例胃癌組織中UbcH10、Ki-67及人錶皮生長因子受體2的錶達.用Real-time PCR和Western blot檢測43例胃癌新鮮標本中UbcH10 mRNA和蛋白的含量.結果 本組117例胃癌組織中UbcH10蛋白的暘性率為83％(97/117),癌徬正常胃黏膜組織中暘性率為55％(64/117),兩者相比差異具有統計學意義(Rs=-0.397,P＜0.01).胃癌組織中UbcH 10蛋白的錶達與患者的性彆、年齡、腫瘤部位、腫瘤直徑無關(Rs =0.001、-0.084、0.052、-0.156,均P＞0.05),但與腫瘤分化、浸潤深度、淋巴結轉移及TNM分期相關(Rs =0.369、-0.378、-0.276、0.421,均P＜0.05).UbcH10與增殖抗原Ki-67、人錶皮生長因子受體2錶達呈正相關(Rs =0.834、0.281,均P＜0.01).胃癌組織中UbcH10 mRNA的錶達量高于癌徬正常組織(△Ct=32.2±5.19、△Ct=4.69±1.26,t=26.8,P＜0.01),UbcH10蛋白在胃癌組織中的錶達水平高于癌徬正常組織(0.74±0.45、0.11±0.04,t=8.09,P＜0.01).UbcH10暘性錶達胃癌患者5年生存率低于UbcH10陰性錶達患者(x2=13.116,P＜0.01).結論 UbcH10在胃癌組織中錶達增彊,UbcH10的錶達可能與胃癌患者的預後有關.
목적 연구위암조직중범소우련매UbcH 10적표체여위암병리분급、림상분기급예후지간적관계.방법 채용면역조직화학방법검측유완정수방자료적117례위암조직중UbcH10、Ki-67급인표피생장인자수체2적표체.용Real-time PCR화Western blot검측43례위암신선표본중UbcH10 mRNA화단백적함량.결과 본조117례위암조직중UbcH10단백적양성솔위83％(97/117),암방정상위점막조직중양성솔위55％(64/117),량자상비차이구유통계학의의(Rs=-0.397,P＜0.01).위암조직중UbcH 10단백적표체여환자적성별、년령、종류부위、종류직경무관(Rs =0.001、-0.084、0.052、-0.156,균P＞0.05),단여종류분화、침윤심도、림파결전이급TNM분기상관(Rs =0.369、-0.378、-0.276、0.421,균P＜0.05).UbcH10여증식항원Ki-67、인표피생장인자수체2표체정정상관(Rs =0.834、0.281,균P＜0.01).위암조직중UbcH10 mRNA적표체량고우암방정상조직(△Ct=32.2±5.19、△Ct=4.69±1.26,t=26.8,P＜0.01),UbcH10단백재위암조직중적표체수평고우암방정상조직(0.74±0.45、0.11±0.04,t=8.09,P＜0.01).UbcH10양성표체위암환자5년생존솔저우UbcH10음성표체환자(x2=13.116,P＜0.01).결론 UbcH10재위암조직중표체증강,UbcH10적표체가능여위암환자적예후유관.
Objective To investigate the expression and significance of UbcH10 in gastric cancer.Methods The expression of UbcH10 in 117 cases of gastric carcinoma tissues,who were followed up postoperatively,were detected by using immunohistochemistry; The expression among different histological grades and clinical stages are analyzed by statistics; Real-time polymerase chain reaction (Rt PCR) and Western blot method were used to detect the expressions of UbcH10 mRNA and protein in 43 cases of gastric carcinoma tissues and the cancer adjacent normal tissues.Results In paraffin fixed blocks of gastric cancer,positive rate of UbcH10 protein expression was higher than normal gastric tissues (83％ vs 55％,Rs =-0.397,P＜0.01).The difference of UbcH10 expression was not significant in gender,age,tumor site and size of the tumor(Rs =0.369,-0.378,-0.276,0.421,P ＜ 0.05),while it was significantly correlated to tumor differentiation,invasive depth,lymphatic metastasis and clinical stage (Rs =0.369,-0.378,-0.276,0.421,P ＜0.05).UbcH10 and Ki-67 protein and HER-2 expression were in significantly positive correlation (Rs =0.834,Rs =0.281,P ＜ 0.01).UbcH10 mRNA and protein expression in fresh gastric carcinoma tissues was higher than that in their corresponding adjacent normal tissues(ACt =32.2±5.19,4.69 ±1.26,t =26.8,P＜0.01),(0.74±0.45,0.11 ±0.04,t =8.09,P＜0.01).The -5 year patient's survival rate in UbcH10 highly-expressed gastric cancer was higher compared to those with UbcH10 low-expression(x2 =13.116,P ＜0.01).Conclusions UbcH10 is upregulated in gastric cancer,and expression of UbcH10 is closely related to the prognosis of patients with gastric cancer.
