CAJ | 학술논문

目的探讨内皮祖细胞(EPC)抑制异基因骨髓移植后肝小静脉血栓形成的作用.方法采用随机数字表法将Balb/c小鼠分为3组,单纯骨髓移植组的小鼠在全身照射后经尾静脉输注C57BL/6小鼠的骨髓单个核细胞5×106/只 ;联合EPC移植组则在单纯骨髓移植的基础上同时输注C57BL/6小鼠的EPC 5×105/只 ;正常对照组的小鼠不作任何处理.分别于移植后第0、5、10、15和20天时,计算各组小鼠的肝脏指数,光镜下观察肝小静脉血栓形成情况以及肝细胞及血管内皮损伤情况,电镜下观察肝小静脉、肝血窦内皮、肝细胞损伤及血小板黏附情况,检测外周血活化血小板的比例及肿瘤坏死因子α(TNF-α)浓度的变化.结果移植后第0、5、10、15和20天时,单纯骨髓移植和联合EPC移植组的活化血小板比例、肝脏指数和外周血TNF-α浓度均呈现上升趋势,至第15天时达到高峰,随后下降,但仍明显高于正常对照组(P＜0.05) ;各时间点联合EPC移植组的上述指标均明显低于单纯骨髓移植组,差异均有统计学意义(P＜0.05).与单纯骨髓移植组比较,联合EPC移植组的血小板黏附减少,肝小静脉血栓形成较少,肝细胞水肿和坏死程度均较轻,且肝脏损伤修复较快.结论小鼠骨髓移植时联合输注EPC能显著抑制肝小静脉血栓形成,明显减轻肝脏损伤.
목적 탐토내피조세포(EPC)억제이기인골수이식후간소정맥혈전형성적작용.방법 채용수궤수자표법장Balb/c소서분위3조,단순골수이식조적소서재전신조사후경미정맥수주C57BL/6소서적골수단개핵세포5×106/지 ;연합EPC이식조칙재단순골수이식적기출상동시수주C57BL/6소서적EPC 5×105/지 ;정상대조조적소서불작임하처리.분별우이식후제0、5、10、15화20천시,계산각조소서적간장지수,광경하관찰간소정맥혈전형성정황이급간세포급혈관내피손상정황,전경하관찰간소정맥、간혈두내피、간세포손상급혈소판점부정황,검측외주혈활화혈소판적비례급종류배사인자α(TNF-α)농도적변화.결과 이식후제0、5、10、15화20천시,단순골수이식화연합EPC이식조적활화혈소판비례、간장지수화외주혈TNF-α농도균정현상승추세,지제15천시체도고봉,수후하강,단잉명현고우정상대조조(P＜0.05) ;각시간점연합EPC이식조적상술지표균명현저우단순골수이식조,차이균유통계학의의(P＜0.05).여단순골수이식조비교,연합EPC이식조적혈소판점부감소,간소정맥혈전형성교소,간세포수종화배사정도균교경,차간장손상수복교쾌.결론 소서골수이식시연합수주EPC능현저억제간소정맥혈전형성,명현감경간장손상.
Objective To (e)xplore inhibition of endothelial progenitor cells (EPCs) against hepatic vein thrombosis after allogeneic bone marrow transplantation (BMT).Methods Balb/c mice were randomly divided into three groups: (1) BMT group [Balb/c mice were injected intravenously with 5 × 106 bone marrow cells after total body irradiation (TBI)]; (2) EPCs co-transfusion with bone marrow cells group: 5 × 105 EPCs were infused into recipient mice simultaneously; (3) Normal control group.Liver index was detected on the day 0,5,10,15 and 20 after transplantation.Hepatic vein thrombosis,hepatic cells and vascular endothelial damage were observed under the light microscopy after H&E staining.The injury of liver cells,liver veins,hepatic sinusoidal endothelial cells (SECs)and platelet adhesion conditions were observed under a transmission electron microscope (TEM).The proportion of activated platelets and TNF-α concentration in peripheral blood were detected by using flow cytometry.Results On the day 0,5,10,15 and 20 after transplantation,the proportion of activated platelets,liver index and TNF-α concentrations in BMT group and EPCs co-transfusion group showed an upward trend,peaked on the 15th day,and then decreased.However,they were still significantly higher than those in normal control group (P＜0.05).The above parameters in EPCs co-transfusion group at each time point were significantly lower than those in BMT group (P＜0.05).As compared with BMT group,platelet adhesion decreased,hepatic vein thromboses were reduced,hepatocyte swelling and necrosis were alleviated,and liver damage repaired rapidly in EPCs co-transfusion group.Conclusion EPCs co-transfusion with bone marrow cells could inhibit the hepatic veins thrombosis and ameliorate liver damage significantly.