中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2012年
11期
680-683
,共4页
周小彪%单忠贵%梁华%邱水玮%林智
週小彪%單忠貴%樑華%邱水瑋%林智
주소표%단충귀%량화%구수위%림지
趋化因子类%CD4+T淋巴细胞%心脏移植%急性排斥反应%小鼠
趨化因子類%CD4+T淋巴細胞%心髒移植%急性排斥反應%小鼠
추화인자류%CD4+T림파세포%심장이식%급성배척반응%소서
Chemotactic factors%CD4-positive T-lyrmphocytes%Heart translpantation%Acute rejection%Mice
目的 探讨趋化因子RANTES在CD4+记忆性T淋巴细胞(Tm细胞)介导的小鼠心脏移植急性排斥反应中表达的变化及意义.方法 以Balb/c小鼠为供鼠,C57BL/6小鼠为受鼠,进行皮肤移植,提取并纯化受鼠脾脏中的CD4+ Tm细胞.分为两组进行实验:实验组,C57BL/6小鼠输注1×106个CD4+ Tm细胞,第2天以Balb/c小鼠为供鼠,进行颈部异位心脏移植；对照组,C57BL/6小鼠未输注CD4 Tm细胞,直接进行心脏移植.观察两组移植心脏存活时间和组织病理学改变,检测移植物中RANTES基因的相对表达量及RANTES在受鼠血清中的浓度.结果 皮肤移植受鼠脾脏中CD4+ Tm细胞达到26.83％.对照组受鼠存活时间为(7.67±0.21)d,实验组为(5.17±0.17) d(P＜0.01).对照组移植心脏急性排斥反应的评级为(2.67±0.14)级,实验组为(3.92±0.08)级(P＜0.01).实验组移植心脏中RANTES基因的相对表达量为对照组的(2.6±0.21)倍(P＜0.01).实验组血清中RANTES浓度为(223.6±16.79) pg/ml,对照组为(120.7±9.47) pg/ml(P＜0.01).结论 接受CD4+ Tm细胞输注的心脏移植受鼠,其体内RANTES的表达量明显增加,加速了急性排斥反应的发生.
目的 探討趨化因子RANTES在CD4+記憶性T淋巴細胞(Tm細胞)介導的小鼠心髒移植急性排斥反應中錶達的變化及意義.方法 以Balb/c小鼠為供鼠,C57BL/6小鼠為受鼠,進行皮膚移植,提取併純化受鼠脾髒中的CD4+ Tm細胞.分為兩組進行實驗:實驗組,C57BL/6小鼠輸註1×106箇CD4+ Tm細胞,第2天以Balb/c小鼠為供鼠,進行頸部異位心髒移植；對照組,C57BL/6小鼠未輸註CD4 Tm細胞,直接進行心髒移植.觀察兩組移植心髒存活時間和組織病理學改變,檢測移植物中RANTES基因的相對錶達量及RANTES在受鼠血清中的濃度.結果 皮膚移植受鼠脾髒中CD4+ Tm細胞達到26.83％.對照組受鼠存活時間為(7.67±0.21)d,實驗組為(5.17±0.17) d(P＜0.01).對照組移植心髒急性排斥反應的評級為(2.67±0.14)級,實驗組為(3.92±0.08)級(P＜0.01).實驗組移植心髒中RANTES基因的相對錶達量為對照組的(2.6±0.21)倍(P＜0.01).實驗組血清中RANTES濃度為(223.6±16.79) pg/ml,對照組為(120.7±9.47) pg/ml(P＜0.01).結論 接受CD4+ Tm細胞輸註的心髒移植受鼠,其體內RANTES的錶達量明顯增加,加速瞭急性排斥反應的髮生.
목적 탐토추화인자RANTES재CD4+기억성T림파세포(Tm세포)개도적소서심장이식급성배척반응중표체적변화급의의.방법 이Balb/c소서위공서,C57BL/6소서위수서,진행피부이식,제취병순화수서비장중적CD4+ Tm세포.분위량조진행실험:실험조,C57BL/6소서수주1×106개CD4+ Tm세포,제2천이Balb/c소서위공서,진행경부이위심장이식；대조조,C57BL/6소서미수주CD4 Tm세포,직접진행심장이식.관찰량조이식심장존활시간화조직병이학개변,검측이식물중RANTES기인적상대표체량급RANTES재수서혈청중적농도.결과 피부이식수서비장중CD4+ Tm세포체도26.83％.대조조수서존활시간위(7.67±0.21)d,실험조위(5.17±0.17) d(P＜0.01).대조조이식심장급성배척반응적평급위(2.67±0.14)급,실험조위(3.92±0.08)급(P＜0.01).실험조이식심장중RANTES기인적상대표체량위대조조적(2.6±0.21)배(P＜0.01).실험조혈청중RANTES농도위(223.6±16.79) pg/ml,대조조위(120.7±9.47) pg/ml(P＜0.01).결론 접수CD4+ Tm세포수주적심장이식수서,기체내RANTES적표체량명현증가,가속료급성배척반응적발생.
Objective To observe the intluence ot chemokine RANTES influence on cardiac allograft acute rejection caused by alloreactive memory CD4+ T cells (Tm) adoptive transfer.Methods Heterotopic heart transplantation (HTx) from Balb/c donors to C57BL/6 recipients was performed by anastomosis of the vessels of the neck.Mice undergoing heterotopic heart transplantation received either adoptive transfer of 1 × 106 CD4+ Tm from the spleen of alloantigen-primed C57BL/6 mice or no cells (control group).After the cardiac transplantation,the mean survival time (MST),mean histologic rank of rejection,relative gene expression and serum concentration of RANTES in the cardiac grafts.Results (1) The percentage of CD4+ Tm was 26.83％ at the spleen of alloantigenprimed mice; (2) The MST was 5.17 ± 0.17 days in the CD4+ Tm+ HTx group versus 7.76 ± 0.21 days at the HTx group (control group) (P＜0.01); (3) The histological tests revealed that mean histologic rank of rejection activity in the sections of cardiac allografts on the day 5 post grafting was grade 3.92 ± 0.08 in the HTx+ CD4+Tm group versus grade 2.67 ± 0.14 in HTx group (P＜0.01) ;(4) The relative gene expression level of RANTES was 2.6 ± 0.21 in the CD4+ Tm + HTx group,significantly higher than in the control group (P＜0.01) ; (5) The serum concentration of RANTES in the CD4+ Tm+ HTx group was 223.6 ± 16.79 pg/mL,higher than in the control group (120.7 ±9.47 pg/mL,P＜0.01).Conclusion Alloreactive CD4+ Tm contribute to the increased expression and secretion of RANTES,and cardiac allograft acute rejection was more extensive in the CD4 + Tm + HTx group.
