中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2013年
7期
419-423
,共5页
朱子玲%吴小津%冯宇锋%戴丽君%常惠荣%吴德沛
硃子玲%吳小津%馮宇鋒%戴麗君%常惠榮%吳德沛
주자령%오소진%풍우봉%대려군%상혜영%오덕패
造血干细胞移植%巨细胞病毒%多态性,单核苷酸
造血榦細胞移植%巨細胞病毒%多態性,單覈苷痠
조혈간세포이식%거세포병독%다태성,단핵감산
Hematopoietic stem cell transplantation%Cytomegalovirus%Polymorphism,single nucleotide
目的 探讨非HLA基因单核苷酸多态性(SNP)对造血干细胞移植后巨细胞病毒(CMV)感染的影响.方法 对2008年7月至2011年12月接受异基因造血干细胞移植的受者及其相应供者64例进行研究.采用聚合酶链反应-序列特异性寡核苷酸探针和测序方法检测髓过氧化物酶基因(MPO)、甘露糖结合凝集素基因(MBL)、CD14基因和血管紧张素转换酶(ACE)基因的SNP,结合CMV pp65检测结果,比较CMV阳性组和CMV阴性组ACE、CD14、MPO、MBL基因SNP的差异.结果 ACE 16内含子基因多态性分布:DD型14例(10.9％),ID型72例(56.3％),Ⅱ型42例(38.8％);CD14-159位基因多态性分布:CC型18例(14.1％),CT型81例(63.3％),TT型29例(22.7％)；MPO-463位点基因多态性分布:G型100例(78.1％),A型2例(1.6％),GA型26例(20.3％)；MBL启动子-550位基因多态性分布:H型28例(21.9％),HL型73例(57.0％),L型27例(21.1％)；MBL启动子-221位基因多态性分布:Y型87例(68.0％),YX型38例(29.7％),X型3例(2.3％)；MBL外显子1区基因多态性分布:A型94例(73.4％),AB型32例(25.0％),B型2例(1.6％).CMV阳性组和CMV阴性组供、受者ACE、CD14-159位点、MPO-463位点、MBL启动子-221以及外显子1区基因单核苷酸多态性相比较,差异均无统计学意义；但CMV阳性组供者MBL-550位点HL基因频率明显增高,与阴性组比较,差异有统计学意义.结论 供者MBL的单核苷酸多态性对造血干细胞移植后CMV感染有影响.
目的 探討非HLA基因單覈苷痠多態性(SNP)對造血榦細胞移植後巨細胞病毒(CMV)感染的影響.方法 對2008年7月至2011年12月接受異基因造血榦細胞移植的受者及其相應供者64例進行研究.採用聚閤酶鏈反應-序列特異性寡覈苷痠探針和測序方法檢測髓過氧化物酶基因(MPO)、甘露糖結閤凝集素基因(MBL)、CD14基因和血管緊張素轉換酶(ACE)基因的SNP,結閤CMV pp65檢測結果,比較CMV暘性組和CMV陰性組ACE、CD14、MPO、MBL基因SNP的差異.結果 ACE 16內含子基因多態性分佈:DD型14例(10.9％),ID型72例(56.3％),Ⅱ型42例(38.8％);CD14-159位基因多態性分佈:CC型18例(14.1％),CT型81例(63.3％),TT型29例(22.7％)；MPO-463位點基因多態性分佈:G型100例(78.1％),A型2例(1.6％),GA型26例(20.3％)；MBL啟動子-550位基因多態性分佈:H型28例(21.9％),HL型73例(57.0％),L型27例(21.1％)；MBL啟動子-221位基因多態性分佈:Y型87例(68.0％),YX型38例(29.7％),X型3例(2.3％)；MBL外顯子1區基因多態性分佈:A型94例(73.4％),AB型32例(25.0％),B型2例(1.6％).CMV暘性組和CMV陰性組供、受者ACE、CD14-159位點、MPO-463位點、MBL啟動子-221以及外顯子1區基因單覈苷痠多態性相比較,差異均無統計學意義；但CMV暘性組供者MBL-550位點HL基因頻率明顯增高,與陰性組比較,差異有統計學意義.結論 供者MBL的單覈苷痠多態性對造血榦細胞移植後CMV感染有影響.
목적 탐토비HLA기인단핵감산다태성(SNP)대조혈간세포이식후거세포병독(CMV)감염적영향.방법 대2008년7월지2011년12월접수이기인조혈간세포이식적수자급기상응공자64례진행연구.채용취합매련반응-서렬특이성과핵감산탐침화측서방법검측수과양화물매기인(MPO)、감로당결합응집소기인(MBL)、CD14기인화혈관긴장소전환매(ACE)기인적SNP,결합CMV pp65검측결과,비교CMV양성조화CMV음성조ACE、CD14、MPO、MBL기인SNP적차이.결과 ACE 16내함자기인다태성분포:DD형14례(10.9％),ID형72례(56.3％),Ⅱ형42례(38.8％);CD14-159위기인다태성분포:CC형18례(14.1％),CT형81례(63.3％),TT형29례(22.7％)；MPO-463위점기인다태성분포:G형100례(78.1％),A형2례(1.6％),GA형26례(20.3％)；MBL계동자-550위기인다태성분포:H형28례(21.9％),HL형73례(57.0％),L형27례(21.1％)；MBL계동자-221위기인다태성분포:Y형87례(68.0％),YX형38례(29.7％),X형3례(2.3％)；MBL외현자1구기인다태성분포:A형94례(73.4％),AB형32례(25.0％),B형2례(1.6％).CMV양성조화CMV음성조공、수자ACE、CD14-159위점、MPO-463위점、MBL계동자-221이급외현자1구기인단핵감산다태성상비교,차이균무통계학의의；단CMV양성조공자MBL-550위점HL기인빈솔명현증고,여음성조비교,차이유통계학의의.결론 공자MBL적단핵감산다태성대조혈간세포이식후CMV감염유영향.
Objective To explore the non-HLA gene polymorphisms that influence CMV infection after hematopoietic stem cell transplantation (HSCT).Method Non-HLA gene (ACE,CD14,MPO,MBL) single nucleotide polymorphisms were determined by using sequence-specific primer polymerase chain reaction (PCR-SSP) and sequencing in 64 pairs of donors and recipients before HSCT and the differences of non-HLA gene were analysed in CMV positive and negative patients Results The distribution of ACE gene single nucleotide polymorphism was DD (14/128,10.9％),ID (72/128,56.3％),and Ⅱ (42/128,38.8％).The distribution of CD14-159 allele gene single nucleotide polymorphism was CC (18/128,14.1％),CT (81/128,63.3％),and TT (29/128,22.7％).The distribution of MPO-463 allele gene single nucleotide polyrnorphism was G (100)/128,78.1％),A (2/128,1.6％),and GA (26/128,20.3％).The distribution of MBL gene single nucleotide polymorphism was H (28/128,21.9％),HL (73/128,57.0％),L (27/128,21.1％),Y (87/128,68.0％),YX (38/128,29.7％),X (3/128,2.3％),A (94/128,73.4％),AB (32/ 128,25.0％),and B (2/128,1.6％).The allele frequency of ACE,CD14 and MPO shoed no significant differcence between CMV positive and negative patients The gene frequency of MBL-HL was increased in CMV positive group.Conclusion MBL gene single nucleotide polymorphisrns may influence CMV infection after HSCT.
