CAJ | 학술논문

目的研究骨髓间充质干细胞(MSC)诱导调节性B淋巴细胞(Breg细胞)的免疫负调节作用.方法取DA大鼠MSC和Lewis大鼠B淋巴细胞共培养后,酶联免疫吸附试验(ELISA)检测MSC和B淋巴细胞混合培养组上清液中白细胞介素10(IL-10)的水平和分泌因子的变化；采用流式细胞技术分析B淋巴细胞增殖、凋亡、表型的变化和对抗原特异性T淋巴细胞增殖的影响,采用激光共聚焦显微镜观察Breg细胞对免疫复合物吞噬能力的变化.结果 MSC成功诱导产生高分泌IL-10的Breg细胞产生,表型为CD19+ CD1d+ CD5+,MSC显著抑制B淋巴细胞的增殖,Breg细胞能抑制抗原特异性T淋巴细胞的增殖,并增加对免疫复合物的吞噬能力.结论 MSC诱导的Breg细胞具有明显的免疫负调节作用.
목적 연구골수간충질간세포(MSC)유도조절성B림파세포(Breg세포)적면역부조절작용.방법 취DA대서MSC화Lewis대서B림파세포공배양후,매련면역흡부시험(ELISA)검측MSC화B림파세포혼합배양조상청액중백세포개소10(IL-10)적수평화분비인자적변화；채용류식세포기술분석B림파세포증식、조망、표형적변화화대항원특이성T림파세포증식적영향,채용격광공취초현미경관찰Breg세포대면역복합물탄서능력적변화.결과 MSC성공유도산생고분비IL-10적Breg세포산생,표형위CD19+ CD1d+ CD5+,MSC현저억제B림파세포적증식,Breg세포능억제항원특이성T림파세포적증식,병증가대면역복합물적탄서능력.결론 MSC유도적Breg세포구유명현적면역부조절작용.
Objective To observe the immunoregulatory roles of MSCs in inducing proliferation of B regulatory cells (Bregs).Method DA Rat MSCs were co-cultured with Wistar rat B cells.The levels of IL-10 and secrete proinflammatory cytokines in the supematants were determined by using ELISA.The proliferation and apoptosis of B cells after interaction with MSCs were analyzed by using FACS.In order to investigate whether Bregs are involved in the phagocytosis of immune complex (IC),B cells and Bregs were incubated with FITC-OVA-IC.The fluorescence intensity was detected by using immunofluorescence microscopy.Results MSCs induced B cells to differentiate into Bregs with high CD19,CD1d and CD5 expression and high IL-10 level.The proliferation of B cells was significantly suppressed after interaction with MSCs.Bregs had enhanced phagocytic capacity as compared with that of B cells.Bregs significantly inhibited maDC-induced CD4+ T cell proliferation compared to B cells,without depressing IL-2 or IFN-γ secretion.Conclusion MSCs induce freshly isolated B cells to differentiate into Bregs characterized by the ability to preferentially produce IL-10.