中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2013年
8期
455-457
,共3页
王庆华%尚乐乐%唐敏英%王瑾%吴琳%谭建明
王慶華%尚樂樂%唐敏英%王瑾%吳琳%譚建明
왕경화%상악악%당민영%왕근%오림%담건명
酶联免疫吸附测定%微球体,荧光%移植物排斥%肾移植
酶聯免疫吸附測定%微毬體,熒光%移植物排斥%腎移植
매련면역흡부측정%미구체,형광%이식물배척%신이식
Enzyme-linked immunosorbent assay%Microspheres,fluorescence%Graft rejection%Kidney transpantation
目的 探讨用Luminex技术与酶联免疫吸附试验(ELISA)方法检测肾移植受者抗HLA抗体的结果差异及其与移植后早期急性排斥反应(AR)的关系.方法 以2010年10月至2012年10月接受亲属活体肾移植的34例受者为研究对象,术前采集受者血清,同时应用Luminex技术与ELISA方法进行抗HLA-Ⅰ类、Ⅱ类抗体检测,并且记录术后早期AR发生情况.结果 用Luminex技术检测抗HLA-Ⅰ类抗体阳性率为41.2％(14/34),检测抗HLA-Ⅱ类抗体阳性率为38.2％(13/34)；用ELISA方法检测抗HLA-Ⅰ类抗体阳性率为2.9％(1/34),检测抗HLA-Ⅱ类抗体阳性率为8.8％(3/34)；两种方法检测抗HLA-Ⅰ类、Ⅱ类抗体阳性率的差异有统计学意义(x2=14.46,P＜0.05;x2 =8.17,P＜0.05).用Luminex技术检测18例术前抗体阳性者中有8例术后早期发生AR(占44.4％)；16例术前抗体阴性者中,有2例术后早期发生AR(占12.5％).用ELISA方法检测4例术前抗体阳性者中,有3例术后早期发生AR(占75.0％)；30例术前抗体阴性者中,有7例术后早期发生AR(占23.3％).结论 Luminex技术检测肾移植受者抗HLA抗体的检出阳性率高于ELISA方法,对于移植后早期AR的监测可以提供有用的信息.
目的 探討用Luminex技術與酶聯免疫吸附試驗(ELISA)方法檢測腎移植受者抗HLA抗體的結果差異及其與移植後早期急性排斥反應(AR)的關繫.方法 以2010年10月至2012年10月接受親屬活體腎移植的34例受者為研究對象,術前採集受者血清,同時應用Luminex技術與ELISA方法進行抗HLA-Ⅰ類、Ⅱ類抗體檢測,併且記錄術後早期AR髮生情況.結果 用Luminex技術檢測抗HLA-Ⅰ類抗體暘性率為41.2％(14/34),檢測抗HLA-Ⅱ類抗體暘性率為38.2％(13/34)；用ELISA方法檢測抗HLA-Ⅰ類抗體暘性率為2.9％(1/34),檢測抗HLA-Ⅱ類抗體暘性率為8.8％(3/34)；兩種方法檢測抗HLA-Ⅰ類、Ⅱ類抗體暘性率的差異有統計學意義(x2=14.46,P＜0.05;x2 =8.17,P＜0.05).用Luminex技術檢測18例術前抗體暘性者中有8例術後早期髮生AR(佔44.4％)；16例術前抗體陰性者中,有2例術後早期髮生AR(佔12.5％).用ELISA方法檢測4例術前抗體暘性者中,有3例術後早期髮生AR(佔75.0％)；30例術前抗體陰性者中,有7例術後早期髮生AR(佔23.3％).結論 Luminex技術檢測腎移植受者抗HLA抗體的檢齣暘性率高于ELISA方法,對于移植後早期AR的鑑測可以提供有用的信息.
목적 탐토용Luminex기술여매련면역흡부시험(ELISA)방법검측신이식수자항HLA항체적결과차이급기여이식후조기급성배척반응(AR)적관계.방법 이2010년10월지2012년10월접수친속활체신이식적34례수자위연구대상,술전채집수자혈청,동시응용Luminex기술여ELISA방법진행항HLA-Ⅰ류、Ⅱ류항체검측,병차기록술후조기AR발생정황.결과 용Luminex기술검측항HLA-Ⅰ류항체양성솔위41.2％(14/34),검측항HLA-Ⅱ류항체양성솔위38.2％(13/34)；용ELISA방법검측항HLA-Ⅰ류항체양성솔위2.9％(1/34),검측항HLA-Ⅱ류항체양성솔위8.8％(3/34)；량충방법검측항HLA-Ⅰ류、Ⅱ류항체양성솔적차이유통계학의의(x2=14.46,P＜0.05;x2 =8.17,P＜0.05).용Luminex기술검측18례술전항체양성자중유8례술후조기발생AR(점44.4％)；16례술전항체음성자중,유2례술후조기발생AR(점12.5％).용ELISA방법검측4례술전항체양성자중,유3례술후조기발생AR(점75.0％)；30례술전항체음성자중,유7례술후조기발생AR(점23.3％).결론 Luminex기술검측신이식수자항HLA항체적검출양성솔고우ELISA방법,대우이식후조기AR적감측가이제공유용적신식.
Objective To compare Luminex vs.ELISA methods in detecting HLA antibodies in kidney transplant recipients and their relation to acute rejection.Method Blood samples from 34 kidney transplant recipients were collected and the HLA antibodies were detected by both Luminex and ELISA methods.The sensitivity and specificity of both methods for predicting the development of acute rejection were analyzed.Results Fourteen recipients (14/34,41.17％) positive for HLA class Ⅰ antibodies were detected by using Luminex method,whereas only 1 case (1/34,2.9％) was detected with positive HLA class Ⅰ antibodies by ELISA method (P＜0.05).Similarly,13 recipients (13/34,38.24％) positive for HLA class Ⅱ antibodies were detected by using Luminex method,whereas the positive rate of HLA class Ⅱ antibodies by using ELISA method was 8.8％ (3/34,P＜0.05).The sensitivity and specificity of Luminex method for predicting the acute rejection were 80％ and 92.3％ respectively,in comparison to 30％ and 77.4％ respectively by ELISA method.Conclusion Compared to the traditional ELISA-based method,Luminex method has a better sensitivity and specificity for predicting the development of acute rejection.
