中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2013年
9期
559-562
,共4页
杨龙%门贺伟%王雅博%诸凯%张雅敏
楊龍%門賀偉%王雅博%諸凱%張雅敏
양룡%문하위%왕아박%제개%장아민
大鼠%肾脏%器官保存%温度%零下非结冰
大鼠%腎髒%器官保存%溫度%零下非結冰
대서%신장%기관보존%온도%령하비결빙
Rats%Kidney%Organ preservation%Temperature%Subzero nonfreezing
目的 寻找保存大鼠肾脏的最适零下非结冰温度,探讨零下非结冰保存大鼠肾脏的效果.方法 使用热电偶探针和温度巡检仪探测大鼠肾脏不同部位的降温曲线,测定出肾脏的冰点.然后将在体灌注的大鼠肾脏取下,并放入盛有肾脏保存液2.5 ml的无菌管中,将其分为6组:-0.8℃组(零下非结冰组)、-0.5℃组(零下非结冰组)、0℃(零度非结冰组)、4℃组(对照组)、-1℃组(零下结冰组)、-4℃组(零下结冰组).低温保存24 h和48 h后,取保存液检测LDH和AST含量,取肾脏上极组织观察病理改变及细胞凋亡情况.结果 肾脏的冰点温度是-1℃,鼠肾的最适零下非结冰保存温度为-0.8℃.零下非结冰保存较传统器官保存温度明显抑制了组织细胞的基础代谢率,减少了因膜损伤而释放的LDH和AST含量,降低了细胞凋亡率[48 h时,-0.8℃组为(40.1±7.0)％,4℃组为(47.1±7.6)％]；保存48 h后,-0.8℃组较其他组的病理改变明显减轻.结论 与0℃到4℃的常规器官保存温度相比,零下非结冰温度(-0.8℃)可明显抑制组织细胞的基础代谢率,减少细胞的势能消耗,降低低温损伤引起的细胞凋亡.
目的 尋找保存大鼠腎髒的最適零下非結冰溫度,探討零下非結冰保存大鼠腎髒的效果.方法 使用熱電偶探針和溫度巡檢儀探測大鼠腎髒不同部位的降溫麯線,測定齣腎髒的冰點.然後將在體灌註的大鼠腎髒取下,併放入盛有腎髒保存液2.5 ml的無菌管中,將其分為6組:-0.8℃組(零下非結冰組)、-0.5℃組(零下非結冰組)、0℃(零度非結冰組)、4℃組(對照組)、-1℃組(零下結冰組)、-4℃組(零下結冰組).低溫保存24 h和48 h後,取保存液檢測LDH和AST含量,取腎髒上極組織觀察病理改變及細胞凋亡情況.結果 腎髒的冰點溫度是-1℃,鼠腎的最適零下非結冰保存溫度為-0.8℃.零下非結冰保存較傳統器官保存溫度明顯抑製瞭組織細胞的基礎代謝率,減少瞭因膜損傷而釋放的LDH和AST含量,降低瞭細胞凋亡率[48 h時,-0.8℃組為(40.1±7.0)％,4℃組為(47.1±7.6)％]；保存48 h後,-0.8℃組較其他組的病理改變明顯減輕.結論 與0℃到4℃的常規器官保存溫度相比,零下非結冰溫度(-0.8℃)可明顯抑製組織細胞的基礎代謝率,減少細胞的勢能消耗,降低低溫損傷引起的細胞凋亡.
목적 심조보존대서신장적최괄령하비결빙온도,탐토령하비결빙보존대서신장적효과.방법 사용열전우탐침화온도순검의탐측대서신장불동부위적강온곡선,측정출신장적빙점.연후장재체관주적대서신장취하,병방입성유신장보존액2.5 ml적무균관중,장기분위6조:-0.8℃조(령하비결빙조)、-0.5℃조(령하비결빙조)、0℃(령도비결빙조)、4℃조(대조조)、-1℃조(령하결빙조)、-4℃조(령하결빙조).저온보존24 h화48 h후,취보존액검측LDH화AST함량,취신장상겁조직관찰병리개변급세포조망정황.결과 신장적빙점온도시-1℃,서신적최괄령하비결빙보존온도위-0.8℃.령하비결빙보존교전통기관보존온도명현억제료조직세포적기출대사솔,감소료인막손상이석방적LDH화AST함량,강저료세포조망솔[48 h시,-0.8℃조위(40.1±7.0)％,4℃조위(47.1±7.6)％]；보존48 h후,-0.8℃조교기타조적병리개변명현감경.결론 여0℃도4℃적상규기관보존온도상비,령하비결빙온도(-0.8℃)가명현억제조직세포적기출대사솔,감소세포적세능소모,강저저온손상인기적세포조망.
Objective To search for the most appropriate subzero nonfreezing temperature,and explore the effect of subzero nonfreezing preservation of rat kidney by comparing with the kidney preservation with conventional temperature (4 ℃,0 ℃) and freezing temperature (-4 ℃).Method The thermocouple probeand the temperature data logging device were used to detect the temperature decreasing curves in different parts of the rat kidney and determine the freezing point of the kidney.The perfused kidneys in rats were removed and put into the sterile tubes containing 2.5 mL hypertonic citrate adenine.Following 6 group were set up:-0.8 ℃ group (subzero nonfreezing),-0.5 ℃group (subzero nonfreezing),0 ℃ group (zero nonfreezing),-4 ℃ group (control group),-1 ℃group(subzero freezing)、-4 ℃ group (subzero freezing).After the cryopreservation for 24 and 48 h,the preservative fluid was harvested for measurement of the contents of LDH and AST,and the paraffin sections from the upper pole of the kidney were made for observation of the pathological changes and apoptosis.Result The freezing temperature of kidney was-1℃ and the most appropriate subzero nonfreezing temperature for preserving the rat kidney was-0.8 ℃.Subzero nonfreezing significantly inhibited the basal metabolic rate of the histiocytes,reduced the contents of LDH and AST released due to the membrane damage,and decreased the apoptosis rate [48 h:-0.8 ℃ (40.1 ± 7.0) ％ vs.4 ℃ (47.1 ± 7.6) ％].Under the light microscope after preservation for 48 h,the pathological changes in-0.8 ℃ group were less than in 4 ℃ group.Conclusion Compared with the organ preservation in conventional temperature (0 ℃-4 ℃),the subzero nonfreezing (-0.8 ℃) can further inhibit the basal metabolic rate of histocytes obviously,reduce its energy consumption,and lower the apoptosis caused by low temperature damage.
