CAJ | 학술논문

目的探讨肝移植后长期生存受者外周血淋巴细胞亚群及细胞因子的特征.方法用流式细胞术及酶联免疫吸附试验法检测肝移植受者(随访时间超过5年以上者为长期组,随访时间在1年以内者为短期组)及健康人(对照组)外周血T淋巴细胞、B淋巴细胞、自然杀伤细胞(NK细胞)、自然杀伤T淋巴细胞(NKT细胞)的变化,并测定细胞因子白细胞介素9(IL-9)、白细胞介素17(IL-17)及白细胞介素22(IL-22)的水平.结果短期组、长期组、对照组的CD3+细胞、CD4+细胞、CD8+细胞百分率及CD8+ CD28+细胞/CD8+细胞值以及IL-9的水平呈逐渐上升趋势.其中,长期组与短期组CD3+细胞百分率及IL-9水平均低于对照组(P＜0.05),短期组CD8+细胞百分率低于对照组(P＜0.05),短期组CD8+ CD28+细胞/CD8+细胞值低于对照组和长期组(P＜0.05).长期组NK细胞及B淋巴细胞百分率高于对照组(P＜0.05),长期组IL-22水平高于短期组及对照组(P＜0.05),2组NKT细胞及IL-17的差异无统计学意义(P＞0.05).结论外周血T淋巴细胞亚群比例及IL-9水平是评价肝移植后受者免疫状态的良好指标,NK细胞、B淋巴细胞及IL-22可能在肝移植术后受者长期生存中发挥着重要角色.
목적 탐토간이식후장기생존수자외주혈림파세포아군급세포인자적특정.방법 용류식세포술급매련면역흡부시험법검측간이식수자(수방시간초과5년이상자위장기조,수방시간재1년이내자위단기조)급건강인(대조조)외주혈T림파세포、B림파세포、자연살상세포(NK세포)、자연살상T림파세포(NKT세포)적변화,병측정세포인자백세포개소9(IL-9)、백세포개소17(IL-17)급백세포개소22(IL-22)적수평.결과 단기조、장기조、대조조적CD3+세포、CD4+세포、CD8+세포백분솔급CD8+ CD28+세포/CD8+세포치이급IL-9적수평정축점상승추세.기중,장기조여단기조CD3+세포백분솔급IL-9수평균저우대조조(P＜0.05),단기조CD8+세포백분솔저우대조조(P＜0.05),단기조CD8+ CD28+세포/CD8+세포치저우대조조화장기조(P＜0.05).장기조NK세포급B림파세포백분솔고우대조조(P＜0.05),장기조IL-22수평고우단기조급대조조(P＜0.05),2조NKT세포급IL-17적차이무통계학의의(P＞0.05).결론 외주혈T림파세포아군비례급IL-9수평시평개간이식후수자면역상태적량호지표,NK세포、B림파세포급IL-22가능재간이식술후수자장기생존중발휘착중요각색.
Objective To explore the characterization of the long-term survival patients through monitoring the lymphoeytes subgroup and cytokines level.Method The lymphocytes subgroups (T,B,and NK) and cytokines (IL-9,-17,-22) of patients and healthy groups were tested by using flow cytometry and ELISA.Results The levels of CD3+,CD4+,CD8+,CD8+ CD28+/CD8+ and IL-9 were gradually increased in the short-term group and long-term group as compared those in healthy group.The percentage of CD3 + cells and level of IL-9 were significantly lower in short-term group and long-term group than in healthy group (P＜0.05).The percentage of CD8+ cell was significantly lower in short-term group than in healthy group (P＜0.05).The ratio of CD8+ CD28+/CD8+ was significantly lower in short-term group than in longterm group and healthy group (P＜0.05).The percentage of B cells and NK cells,and IL-22 were gradually increased in the healthy group,shortterm group and long-term group.The percentage of B cells and NK cells was significamly higher in long-term group than in healthy group (P＜0.05).The level of IL-22 was significantly higher in longterm group than in short-term group and healthy group (P＜0.05).However,NKT lymnphocytes and IL-17 showed no statistically significant difference between long-term group and short-term group.Conclusion The ratio of T lymphocyte subgroups and the level of IL-9 were good biomarkers for evaluating the immune characterization of OLT patients; NK and B lymphocytes,and IL-22 may be associated with the long-term survival in patients after OLT.