CAJ | 학술논문

目的探讨TLR4基因Asp299Gly和Thr399Ile多态性位点在我国部分异基因造血干细胞移植供、受者中的分布.方法以单中心2007-2012年间104对异基因造血干细胞移植供、受者为研究对象,获取了208份供者和受者的外周血样本,通过样本DNA的扩增产物直接测序法和聚合酶链反应-限制性片段多态性技术验证TLR4基因Asp299Gly和Thr399Ile位点的多态性.结果两种方法均未发现样本具有TLR4多态性位点Asp299Gly或Thr399Ile的碱基变异.结论我国部分造血干细胞移植人群中TLR4基因Asp299Gly和Thr399Ile的基因多态性非常罕见.
목적 탐토TLR4기인Asp299Gly화Thr399Ile다태성위점재아국부분이기인조혈간세포이식공、수자중적분포.방법 이단중심2007-2012년간104대이기인조혈간세포이식공、수자위연구대상,획취료208빈공자화수자적외주혈양본,통과양본DNA적확증산물직접측서법화취합매련반응-한제성편단다태성기술험증TLR4기인Asp299Gly화Thr399Ile위점적다태성.결과 량충방법균미발현양본구유TLR4다태성위점Asp299Gly혹Thr399Ile적감기변이.결론 아국부분조혈간세포이식인군중TLR4기인Asp299Gly화Thr399Ile적기인다태성비상한견.
Objective To determine the frequency of Toll-like receptor 4 (TLR4)polymorphisms Asp299Gly and Thr399Ile in a cohort of hematopoietic stem cell transplant recipients and their donors in China.Method We examined the polymorphisms in 208 peripheral blood samples collected from 104 recipients and their donors in a single center between 2007-2012 in Zhejiang Province,China,and Asp299Gly and Thr399Ile TLR4 gene polymorphisms were detected using the sample DNA amplification products direct sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Result Both methods didn't demonstrate TLR4 polymorphisms Asp299Gly or Thr399Ile base mutation in our samples.Conclusion The TLR4 Asp299Gly and Thr399Ile polymorphisms are very rare in our part of the population of hematopoietic stem cell transplantation.