CAJ | 학술논문

目的研究他克莫司血药浓度对肾移植受者血脂的影响及其与G蛋白β3亚单位(GNB3)基因825T等位基因(GNB3 C825T)多态性的相关性.方法 81例肾移植受者依据他克莫司浓度分为普通浓度组(31例)和低浓度组(50例),免疫抑制方案均为联合应用甲泼尼龙(MP)、他克莫司、吗替麦考酚酯(MMF)及巴利昔单抗.统计受者移植后1、3、6和12个月的血清总胆固醇和甘油三脂水平;并且利用快速的序列特异性聚合酶链反应方法检测人GNB3 C825T单核苷酸多态性,分析GNB3 C825T多态性与他克莫司治疗后高血脂发生率的关系.结果两组术前总胆固醇和甘油三酯水平的差异无统计学意义(P＞0.05),排除发生急性排斥反应的受者后,两组术后3、6、12个月时甘油三脂水平的差异有统计学意义(P＜0.05),两组血胆固醇水平的差异无统计学意义(P＞0.05).81例中,T/C基因型者高甘油三脂血症发生率为22.0％ (9/41),C基因型者为27.3％(6/22),T基因型者为33.3％(6/18),高甘油三脂血症发生情况与GNB3基因多态性无明显相关(P=0.647).结论他克莫司影响肾移植术后的甘油三酯水平,与其浓度正相关;高甘油三脂血症发生率与GNB3 C825T单核苷酸多态性相关不显著.
목적 연구타극막사혈약농도대신이식수자혈지적영향급기여G단백β3아단위(GNB3)기인825T등위기인(GNB3 C825T)다태성적상관성.방법 81례신이식수자의거타극막사농도분위보통농도조(31례)화저농도조(50례),면역억제방안균위연합응용갑발니룡(MP)、타극막사、마체맥고분지(MMF)급파리석단항.통계수자이식후1、3、6화12개월적혈청총담고순화감유삼지수평;병차이용쾌속적서렬특이성취합매련반응방법검측인GNB3 C825T단핵감산다태성,분석GNB3 C825T다태성여타극막사치료후고혈지발생솔적관계.결과 량조술전총담고순화감유삼지수평적차이무통계학의의(P＞0.05),배제발생급성배척반응적수자후,량조술후3、6、12개월시감유삼지수평적차이유통계학의의(P＜0.05),량조혈담고순수평적차이무통계학의의(P＞0.05).81례중,T/C기인형자고감유삼지혈증발생솔위22.0％ (9/41),C기인형자위27.3％(6/22),T기인형자위33.3％(6/18),고감유삼지혈증발생정황여GNB3기인다태성무명현상관(P=0.647).결론 타극막사영향신이식술후적감유삼지수평,여기농도정상관;고감유삼지혈증발생솔여GNB3 C825T단핵감산다태성상관불현저.
Objective To study the effect of Tacrolimus on blood lipid after renal transplantation,and the relationship between C825T polymorphism in G protein beta 3 subunit (GNB3) gene and serum lipid levels.Method Eighty-one cases of recipients patients after renal transplantation were divided into two groups in terms of Tacrolimus concentration:normal blood concentration group (group A) and low blood concentration group (group B).The serum lipid levels at 1st,3rd,6th,and 12th month after renal transplantation were measured.Genotype was determined by the simple sequence-specific primer polymerase chain reaction (SSP-PCR).Result The percentage of patients with hypertriglyceride in group A was significantly higher than in group B during the one-year follow-up period.There was significant difference between the two groups in the serum triglyceride levels but no difference in the serum cholesterol levels.The 825C/T polymorphism in the GNB3 gene was not associated with hypertriglyceride in renal transplantations in Wenzhou.Conclusion The serum triglyceride levels in renal transplantations in Wenzhou was associated with the Tacrolimus concentration,and the incidence of hypertriglyceride is not associated with the 825C/T polymorphism in the GNB3 gene.