中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2014年
4期
243-246
,共4页
刘争%张洁元%段朝霞%陈慧俊%余华荣%张路%李兵仓
劉爭%張潔元%段朝霞%陳慧俊%餘華榮%張路%李兵倉
류쟁%장길원%단조하%진혜준%여화영%장로%리병창
大鼠%脊髓损伤%表皮神经嵴干细胞%细胞移植%胶质细胞源性神经营养因子
大鼠%脊髓損傷%錶皮神經嵴榦細胞%細胞移植%膠質細胞源性神經營養因子
대서%척수손상%표피신경척간세포%세포이식%효질세포원성신경영양인자
Rats%Spinal cord injury%Epidermal neural crest stem cell%Cell transplantation%Glial cell line-derived neurotrophic factor
目的 探讨表皮神经嵴干细胞(EPI-NCSC)移植对大鼠损伤脊髓修复过程中胶质细胞源性神经营养因子(GDNF)表达的影响.方法 分离培养绿色荧光蛋白转基因大鼠的EPI-NCSC,备移植用.取30只SD大鼠,暴露T10脊髓并在NYU-Ⅱ撞击机下以10 g×25 mm的致伤力挫伤脊髓建立脊髓损伤模型,大鼠分为空白损伤组、DMEM对照组和实验组.脊髓损伤后1周,将EPI-NCSC悬液注射入大鼠损伤的脊髓处,DMEM对照组用单纯的DMEM/F12培养液代替,空白损伤组未处理.脊髓损伤后每周进行1次BBB评分以评估大鼠的运动功能,移植后6周取材,检测GDNF mRNA及GDNF蛋白的表达.结果 实验组大鼠的BBB评分从移植后第2周开始就显著高于其他两组,差异均有统计学意义(P＜0.05).实验组损伤的脊髓组织处GDNFmRNA和蛋白的表达均明显高于其他两组,差异均有统计学意义(P＜0.05).而空白损伤组和DMEM对照组间BBB评分及GDNFmRNA和蛋白表达量均无明显差异(P＞0.05).结论 EPI-NCSC移植后,EPI-NCSC能促进大鼠脊髓组织GDNF的表达,从而有助于修复大鼠损伤的脊髓.
目的 探討錶皮神經嵴榦細胞(EPI-NCSC)移植對大鼠損傷脊髓脩複過程中膠質細胞源性神經營養因子(GDNF)錶達的影響.方法 分離培養綠色熒光蛋白轉基因大鼠的EPI-NCSC,備移植用.取30隻SD大鼠,暴露T10脊髓併在NYU-Ⅱ撞擊機下以10 g×25 mm的緻傷力挫傷脊髓建立脊髓損傷模型,大鼠分為空白損傷組、DMEM對照組和實驗組.脊髓損傷後1週,將EPI-NCSC懸液註射入大鼠損傷的脊髓處,DMEM對照組用單純的DMEM/F12培養液代替,空白損傷組未處理.脊髓損傷後每週進行1次BBB評分以評估大鼠的運動功能,移植後6週取材,檢測GDNF mRNA及GDNF蛋白的錶達.結果 實驗組大鼠的BBB評分從移植後第2週開始就顯著高于其他兩組,差異均有統計學意義(P＜0.05).實驗組損傷的脊髓組織處GDNFmRNA和蛋白的錶達均明顯高于其他兩組,差異均有統計學意義(P＜0.05).而空白損傷組和DMEM對照組間BBB評分及GDNFmRNA和蛋白錶達量均無明顯差異(P＞0.05).結論 EPI-NCSC移植後,EPI-NCSC能促進大鼠脊髓組織GDNF的錶達,從而有助于脩複大鼠損傷的脊髓.
목적 탐토표피신경척간세포(EPI-NCSC)이식대대서손상척수수복과정중효질세포원성신경영양인자(GDNF)표체적영향.방법 분리배양록색형광단백전기인대서적EPI-NCSC,비이식용.취30지SD대서,폭로T10척수병재NYU-Ⅱ당격궤하이10 g×25 mm적치상력좌상척수건립척수손상모형,대서분위공백손상조、DMEM대조조화실험조.척수손상후1주,장EPI-NCSC현액주사입대서손상적척수처,DMEM대조조용단순적DMEM/F12배양액대체,공백손상조미처리.척수손상후매주진행1차BBB평분이평고대서적운동공능,이식후6주취재,검측GDNF mRNA급GDNF단백적표체.결과 실험조대서적BBB평분종이식후제2주개시취현저고우기타량조,차이균유통계학의의(P＜0.05).실험조손상적척수조직처GDNFmRNA화단백적표체균명현고우기타량조,차이균유통계학의의(P＜0.05).이공백손상조화DMEM대조조간BBB평분급GDNFmRNA화단백표체량균무명현차이(P＞0.05).결론 EPI-NCSC이식후,EPI-NCSC능촉진대서척수조직GDNF적표체,종이유조우수복대서손상적척수.
Objective To explore the expression of glial cell line-derived neurotrophic factor (GDNF) in rats with spinal cord injury (SCI) after epidermal neural crest stem cells (EPI-NCSCs) transplantation.Method EPI-NCSCs were isolated from GFP transgenic rats for transplantation.The rat SCI model was made by NYU-II impactor (10 g 25 mm) at T10 level.Then 30 SD rats were randomly divided into blank injury group (group A),DMEM transplantation group (group B),and experimental group (group C).The EPI-NCSCs were transplanted into the injured region one week after SCI.In DMEM group,the DMEM/F12 was used to substitute for the EPI-NCSCs.No treatment was done in blank injury group.The locomotor function was appraised by BBB score every week after transplantation.At sixth week after transplantation,GDNF mRNA and protein expression was detected.Result The BBB score in experimental group was significantly higher than the other two groups from two weeks after transplantation (P＜0.05).The expression of GDNF mRNA and protein in experimental group was significantly higher than the other two groups (P＜0.05).There was no significant difference between blank injury group and DMEM transplantation group (P ＞ 0.05).Conclusion The expression of GDNF can be up-regulated by EPI-NCSCs transplantation,which may be one of the mechanisms for EPI-NCSCs repairing SCI.
