CAJ | 학술논문

目的对比3种人主要组织相容性复合物Ⅰ类相关链A位点(MICA)特异性抗体检测试剂结果的一致性,预测其临床推广应用的价值.方法 18家实验室参加16届国际组织相容性和免疫协作组,均采用免疫磁珠流式细胞仪液相芯片技术检测4批16份血清样本的MICA抗体的特异性,3种试剂标记为Kit-A、Kit-B、Kit-C.结果在16份血清样本中,S04为阴性,15份样本结果阳性,其中S10样本各实验室均上报为阳性.依据MICA抗原公共表位分G1 (MICA 01、02、07、12、17、18)组和G2 (MICA 04、06、08/27、09、19)组,5例样本用Kit-A和Kit-B均能判别MICA-G1组特异性抗体,Kit-C存在MICA 08/27和MICA 19的假阳性结果.5例样本用Kit-A和Kit-B均能判别MICA-G2组特异性抗体.其余5例样本用Kit-A、Kit-B和Kit-C均无法判别MICA特异性抗体一致的结果.从3种试剂均能判别10种MICA特异性抗体结果分析,MICA08/27(86.67％,13/15)抗体结果一致性最高,MICA19(40％,6/15)一致性最低.使用Kit-B试剂的实验室间有13例MICA特异性抗体样本结果符合率大于80％.结论协作组实验室对于判断MICA抗体阴性或阳性没有差异,而对MICA抗体特异性分析存在不一致性,因此,建议选择MICA特异性抗体类型较宽检测试剂或最好选择两种以上的试剂进行检测.
목적 대비3충인주요조직상용성복합물Ⅰ류상관련A위점(MICA)특이성항체검측시제결과적일치성,예측기림상추엄응용적개치.방법 18가실험실삼가16계국제조직상용성화면역협작조,균채용면역자주류식세포의액상심편기술검측4비16빈혈청양본적MICA항체적특이성,3충시제표기위Kit-A、Kit-B、Kit-C.결과 재16빈혈청양본중,S04위음성,15빈양본결과양성,기중S10양본각실험실균상보위양성.의거MICA항원공공표위분G1 (MICA 01、02、07、12、17、18)조화G2 (MICA 04、06、08/27、09、19)조,5례양본용Kit-A화Kit-B균능판별MICA-G1조특이성항체,Kit-C존재MICA 08/27화MICA 19적가양성결과.5례양본용Kit-A화Kit-B균능판별MICA-G2조특이성항체.기여5례양본용Kit-A、Kit-B화Kit-C균무법판별MICA특이성항체일치적결과.종3충시제균능판별10충MICA특이성항체결과분석,MICA08/27(86.67％,13/15)항체결과일치성최고,MICA19(40％,6/15)일치성최저.사용Kit-B시제적실험실간유13례MICA특이성항체양본결과부합솔대우80％.결론 협작조실험실대우판단MICA항체음성혹양성몰유차이,이대MICA항체특이성분석존재불일치성,인차,건의선택MICA특이성항체류형교관검측시제혹최호선택량충이상적시제진행검측.
Objective To research the consistency of testing results with three different antimajor histocompatibility complex class Ⅰ-related chain A(MICA) specific antibody reagents in order to evaluate their clinical application's value.Method An collaborative study of 18 laboratories was undertaken at the 16th International HLA and Irnmunogenetics Workshop.Total of 16 sera(4 batchs)were tested for anti-MICA antibodies by Luminex method with three different reagents (Kit-A,-B and -C).Result Anti-MICA antibodies were found in 15 sera,except one sera(no.S04) ; No.S10 sera showed positive results in all the laboratories.The anti-MICA antibodies were divided into MICA-G1 group (MICA01,02,07,12,17 and 18) and MICA-G2 group (MICA 04,06,08/27,09 and 19).MICA-G1 group specific antibodies were detected in 5 sera with Kit-A and-B reagent; but there were false-positive results of anti-MICA08/27 and MICA19 antibodies in this 5 sera with Kit-C.MICA-G2group specific antibodies can be detected in other 5 sera with Kit-A and-B,But the MICA specific antibodies testing gave different results with Kit-A,-B and-C in all the last 5 sera samples.Testing of MICA08/27 showed highest consistency results (86.67％,13/15) with Kit-A,-B and-C; and testing of MICA19 showed lowest consistency results (40％,6/15) with this 3 reagents.There were 80％ consistency results of anti-MICA specific antibodies in 13 sera with Kit-B.Conclusion There are the same effect to judgment positive or negative result for anti-MICA antibodies with 3 different reagents,but the results of anti-MICA specific antibodies are not the same.Therefore,it's better to use two or more reagents to test anti-MICA specific antibodies,or choose reagent with wide detection range.