CAJ | 학술논문

目的研究丹酚酸B对大鼠离体供肺的保护作用.方法将SD大鼠24只采用简单随机法分为对照组和实验组,每组各12只.对照组选择棉籽糖低钾右旋糖酐液(R-LPD液)灌注肺脏,实验组在R-LPD液中加入丹酚酸B注射液,800 mg/L.建立大鼠离体肺冷保存模型.肺脏灌注保存6、9、12和24 h后分别测两组肺组织湿肺质量/干肺质量(W/D)、髓过氧化物酶(MPO)活性、丙二醛含量、超氧化物歧化酶(SOD)活性,并在光镜下观察肺组织超微结构.结果两组内9h和6h、12 h和9h时间点之间比较,W/D、丙二醛含量、MPO活性和SOD活性的差异无统计学意义(P＞0.05);两组内24 h时各参数水平明显高于12 h时(P＜0.01).两组间相比较,6、9和12 h时各参数的差异无统计学意义(P＞0.05).保存至24 h时,对照组W/D为4.322±0.364,高于实验组的4.065±0.160(P＜0.05);对照组丙二醛含量为0.519±0.091,高于对照组的0.484±0.077 (P＜0.05);对照组MPO为(0.824±0.033)U/g,高于对照组的(0.798±0.030)U/g;而SOD活性对照组为109.5±17.1,低于实验组的122.2±9.2(P＜0.05).光镜下两组肺组织保存至12 h以前,病理形态学均无明显炎症损伤,仅表现为轻度炎症反应.12 h后对照组肺组织出现肺泡结构破坏、间质水肿、炎症损伤较实验组明显.结论加入丹酚酸B后的灌注液在肺脏保存12 h前未有明显优势,12 h后能更加有效地保证供肺质量,使供者肺在离体情况下有效保存更长的时间;丹酚酸B有可能在肺移植中对供者肺的保护发挥作用.
목적 연구단분산B대대서리체공폐적보호작용.방법 장SD대서24지채용간단수궤법분위대조조화실험조,매조각12지.대조조선택면자당저갑우선당항액(R-LPD액)관주폐장,실험조재R-LPD액중가입단분산B주사액,800 mg/L.건립대서리체폐랭보존모형.폐장관주보존6、9、12화24 h후분별측량조폐조직습폐질량/간폐질량(W/D)、수과양화물매(MPO)활성、병이철함량、초양화물기화매(SOD)활성,병재광경하관찰폐조직초미결구.결과 량조내9h화6h、12 h화9h시간점지간비교,W/D、병이철함량、MPO활성화SOD활성적차이무통계학의의(P＞0.05);량조내24 h시각삼수수평명현고우12 h시(P＜0.01).량조간상비교,6、9화12 h시각삼수적차이무통계학의의(P＞0.05).보존지24 h시,대조조W/D위4.322±0.364,고우실험조적4.065±0.160(P＜0.05);대조조병이철함량위0.519±0.091,고우대조조적0.484±0.077 (P＜0.05);대조조MPO위(0.824±0.033)U/g,고우대조조적(0.798±0.030)U/g;이SOD활성대조조위109.5±17.1,저우실험조적122.2±9.2(P＜0.05).광경하량조폐조직보존지12 h이전,병리형태학균무명현염증손상,부표현위경도염증반응.12 h후대조조폐조직출현폐포결구파배、간질수종、염증손상교실험조명현.결론 가입단분산B후적관주액재폐장보존12 h전미유명현우세,12 h후능경가유효지보증공폐질량,사공자폐재리체정황하유효보존경장적시간;단분산B유가능재폐이식중대공자폐적보호발휘작용.
Objective To explore the protective effect of Salvianolic acid B on isolated lung in rats.Method Twenty-four SD rats were randomly divided into 2 groups:control group and experimental group (n =12 each).The isolated lung in control group was perfused with raffinose-low potassium dextran (R-LPD),and that in experimental group was perfused with R-LPD and 800 mg/L of Salvianolic acid B.The model of isolated lung was established in all these rats.Wet/dry weight ratio (W/D),myeloperoxidase (MPO),malondialdehyde (MDA) and superoxide dismutase (SOD) were measured,and the pulmonary structures were observed by HE staining at different preservative periods of 6,9,12 and 24 h after infusion.Result Intragroup comparison was made in both groups:all parameters had no statistically significant difference before 12 h.However,W/D,MPO,MDA,and SOD at 24 h were higher than those at 12 h (P＜0.01).The result of interclass comparison showed that after 6,9 and 12 h preservation,all parameters of these two groups showed no significant difference.However,W/D,MDA and MPO were lower,and SOD was higher after 24 h preservation in control group (P＜0.05).Moreover,before 12 h preservation,the two groups did not show inflammatory injury,only manifested slight inflammatory reaction histologically.Destructive pulmonary structure,alveolar interstitial edema and inflammatory damage were seen more clearly in control group than in experimental group.Conclusion The perfusion with Salvianolic acid B had no apparent superiority before 12 h.However,after 12 h,it could ensure the quality and prolong the preservation time of the donor lungs more effectively.Salvianolic acid B might play an important role in donor lungs protection.