CAJ | 학술논문

目的探讨颈髓1～2后角星形胶质细胞对偏头痛大鼠模型痛觉超敏的影响.方法 54只SD大鼠按随机数字表法分为9组:空白组、假手术组、新型致炎剂炎性汤(inflammatory soup,IS)3d组(IS3 d组)、新型致炎剂7d组(IS7 d组)、生理盐水组、氟代柠檬酸(FCA)预防组(IS-FCA预防组)、FCA预防对照组(IS-NS预防组)、FCA治疗组(IS-FCA治疗组)和FCA治疗对照组(IS-NS治疗组).采用新型致炎剂刺激大鼠硬脑膜模型,应用Von-Frey纤维丝测定大鼠眶周皮肤的痛觉阈值,免疫荧光技术观察各组大鼠颈髓1～2后角神经元激活标志物C-fos和星形胶质细胞特异性蛋白胶质纤维酸性蛋白(GFAP)的表达.结果 ①Von-Frey结果:与对照组阈值相比,IS3 d组、IS7 d组疼痛阈值下降明显(分别为3d组:6.4±0.3与5.3±0.3,F=40.15；7d组:6.3±0.2与3.0±0.3,F=382.5,均P＜0.01),IS-FCA预防组、IS-FCA治疗组疼痛阈值均在给予FCA第4天明显升高(预防组:5.5±5.1与5.1±0.2,F=16.00；治疗组:4.3±0.2与3.0±0.2,F=138.0,均P＜0.01).②GFAP免疫荧光:IS3 d、IS7 d组高于对照组(吸光度值分别为:3 d:24.5±4.4与14.8±2.5,F=32.54;7 d:38.9±7.1与14.6±1.8,F=63.56,均P＜0.01).③C-fos免疫荧光:IS3 d组较对照组增高(20.4±2.3与8.4±1.1,F=129.0,P＜0.01),IS7 d组与对照组差异无统计学意义.结论在慢性硬脑膜炎症所致的偏头痛痛觉超敏中,我们发现了星形胶质细胞的激活,且其特异性抑制剂FCA能有效预防并缓解痛觉超敏,提示星形胶质细胞可能在启动和维持痛觉超敏阶段均发挥着重要作用.
목적 탐토경수1～2후각성형효질세포대편두통대서모형통각초민적영향.방법 54지SD대서안수궤수자표법분위9조:공백조、가수술조、신형치염제염성탕(inflammatory soup,IS)3d조(IS3 d조)、신형치염제7d조(IS7 d조)、생리염수조、불대저몽산(FCA)예방조(IS-FCA예방조)、FCA예방대조조(IS-NS예방조)、FCA치료조(IS-FCA치료조)화FCA치료대조조(IS-NS치료조).채용신형치염제자격대서경뇌막모형,응용Von-Frey섬유사측정대서광주피부적통각역치,면역형광기술관찰각조대서경수1～2후각신경원격활표지물C-fos화성형효질세포특이성단백효질섬유산성단백(GFAP)적표체.결과 ①Von-Frey결과:여대조조역치상비,IS3 d조、IS7 d조동통역치하강명현(분별위3d조:6.4±0.3여5.3±0.3,F=40.15；7d조:6.3±0.2여3.0±0.3,F=382.5,균P＜0.01),IS-FCA예방조、IS-FCA치료조동통역치균재급여FCA제4천명현승고(예방조:5.5±5.1여5.1±0.2,F=16.00；치료조:4.3±0.2여3.0±0.2,F=138.0,균P＜0.01).②GFAP면역형광:IS3 d、IS7 d조고우대조조(흡광도치분별위:3 d:24.5±4.4여14.8±2.5,F=32.54;7 d:38.9±7.1여14.6±1.8,F=63.56,균P＜0.01).③C-fos면역형광:IS3 d조교대조조증고(20.4±2.3여8.4±1.1,F=129.0,P＜0.01),IS7 d조여대조조차이무통계학의의.결론 재만성경뇌막염증소치적편두통통각초민중,아문발현료성형효질세포적격활,차기특이성억제제FCA능유효예방병완해통각초민,제시성형효질세포가능재계동화유지통각초민계단균발휘착중요작용.
Objective To explore the effects of astrocytes in cervical spinal cord posterior horn on allodynia in rat models of migraine.Methods Fifty-four male SD rats were randomly divided into 9 groups (each group n =6): blank group,sham surgery group,new inflammatory soup 3 d group (IS 3 d group),new inflammatory soup 7 d group(IS 7 d group),saline group,fluorocitrate (FCA)prevention group,FCA prevention control group,FCA treatment group,FCA treatment control group.Following the IS stimulation in the rat dual matter,Von-Frey hair was used to monitor the pain threshold of periorbital skin.Immunofluorescence technique was used to observe the expression of specific marker of astrocyte,glial fibrilary acidic protein (GFAP),and neuron activation marker,C-fos.Results ①Von-Frey hair study showed the thresholds of IS 3 d group and IS 7 d group were significantly decreased compared with the control group(3 d:5.3 ±0.3 vs 6.4 ±0.3,F =40.15;7 d:3.0 ±0.3 vs 6.3 ±0.2,F =382.5,both P ＜0.01).The pain thresholds of FCA prevention group and FCA treatment group both began to significantly increase at the day 4 (FCA prevention:5.5 ± 5.1 vs 5.1 ± 0.2,F =16.00 ;FCA treatment:4.3 ± 0.2 vs 3.0 ± 0.2,F =138.0,both P ＜ 0.01).②GFAP immunofluorescence showed that the mean optical density (A) values of IS 3 d group and IS 7 d group were significantly increased compared with the control group(3 d:24.5 ±4.4 vs 14.8 ± 2.5,F =32.54;7 d:38.9 ± 7.1 vs 14.6 ± 1.8,F =63.56,both P ＜ 0.01).()C-fosimmunofluorescence showed that mean A value of IS 3 d group was significantly increased compared with the control group (20.4 ± 2.3 vs 8.4 ± 1.1,F =129.0,P ＜ 0.01).The difference was not significant between the IS 7 d group and the control group.Conclusions Activated astrocytes contribute to the facial allodynia induced by chronic dural inflammation.Its inhibitor FCA could both prevent and treat the allodynia behaviour.All of these suggest that astrocytes may not only contribute to the initiation of mechanical allodynia but also the maintenance.