CAJ | 학술논문

目的探讨C反应蛋白-757T/C基因多态性与缺血性脑血管病患者颈动脉粥样硬化的相关性.方法采用病例对照研究的方法,对276例缺血性脑血管病患者及125名对照者(两组成员性别、年龄均匹配)应用聚合酶链反应-限制性片段长度多态性对C反应蛋白-757T/C基因位点进行分析,采用免疫散射比浊法测定血清C反应蛋白水平,应用彩色多普勒超声测定缺血性脑血管病患者颈总动脉及颈内动脉内膜-中膜厚度(IMT),分析C反应蛋白-757T/C基因型、等位基因型与颈动脉粥样硬化的关系.结果缺血性脑血管病组患者C反应蛋白-757T/C基因型TF、TC和CC的频率分布[207/276(75.0％)、65/276(23.6％)和4/276(1.4％)]与对照组[75/125(60.0％)、46/125(36.8％)和4/125(3.2％)]比较,差异具有统计学意义(x2=9.531,P=0.008)；缺血性脑血管病患者C反应蛋白-757T等位基因频率[479/552(86.8％)]高于对照组[196/250(78.4％)],而C等位基因频率低于对照组[73/552(13.2％)与54/250(21.6％)],差异具有统计学意义(x2=9.056,P=0.004);-757TC/CC基因型患者血清C反应蛋白水平较TT基因型患者明显增高[(4.2±1.6)mg/L与(2.7±1.9)mg/L;t =5.900,P＜0.01]；Logistic回归分析表明年龄、糖尿病、血清C反应蛋白水平及低密度脂蛋白胆固醇是颈动脉IMT增厚的独立的危险因素,并且C反应蛋白-757多态位点C等位基因与颈动脉粥样硬化具有相关性(OR=1.379,95％ CI 0.852 ～0.864,P=0.023).结论 C反应蛋白-757T/C基因多态性与缺血性脑血管病有关,C反应蛋白-757C等位基因能够影响血清C反应蛋白水平；Logistic回归分析表明C反应蛋白-757T/C基因多态性与颈动脉粥样硬化相关.
목적 탐토C반응단백-757T/C기인다태성여결혈성뇌혈관병환자경동맥죽양경화적상관성.방법 채용병례대조연구적방법,대276례결혈성뇌혈관병환자급125명대조자(량조성원성별、년령균필배)응용취합매련반응-한제성편단장도다태성대C반응단백-757T/C기인위점진행분석,채용면역산사비탁법측정혈청C반응단백수평,응용채색다보륵초성측정결혈성뇌혈관병환자경총동맥급경내동맥내막-중막후도(IMT),분석C반응단백-757T/C기인형、등위기인형여경동맥죽양경화적관계.결과 결혈성뇌혈관병조환자C반응단백-757T/C기인형TF、TC화CC적빈솔분포[207/276(75.0％)、65/276(23.6％)화4/276(1.4％)]여대조조[75/125(60.0％)、46/125(36.8％)화4/125(3.2％)]비교,차이구유통계학의의(x2=9.531,P=0.008)；결혈성뇌혈관병환자C반응단백-757T등위기인빈솔[479/552(86.8％)]고우대조조[196/250(78.4％)],이C등위기인빈솔저우대조조[73/552(13.2％)여54/250(21.6％)],차이구유통계학의의(x2=9.056,P=0.004);-757TC/CC기인형환자혈청C반응단백수평교TT기인형환자명현증고[(4.2±1.6)mg/L여(2.7±1.9)mg/L;t =5.900,P＜0.01]；Logistic회귀분석표명년령、당뇨병、혈청C반응단백수평급저밀도지단백담고순시경동맥IMT증후적독립적위험인소,병차C반응단백-757다태위점C등위기인여경동맥죽양경화구유상관성(OR=1.379,95％ CI 0.852 ～0.864,P=0.023).결론 C반응단백-757T/C기인다태성여결혈성뇌혈관병유관,C반응단백-757C등위기인능구영향혈청C반응단백수평；Logistic회귀분석표명C반응단백-757T/C기인다태성여경동맥죽양경화상관.
Objective To explore the relationship of a polymorphism c.757T ＞ C in C-reactive protein gene (CRP) and carotid artery atherosclerosis in patients with ischemic cerebrovascular disease (ICVD).Methods A case-control study investigated 276 ICVD patients and 125 controls matched for age and sex.Genotypes of-757T/C in CRP were analyzed in patients with ICVD using polymerase chain reaction-restriction fragment length polymorphism.The level of serum C-reactive protein was detected by using immune scatter turbidimetry and the intima-media thickness of common carotid artery and internal carotid artery were measured by color Doppler ultrasonography.The relationship between the polymorphism -757T/C in CRP and carotid atherosclerosis was assessed.Results The genotypes of the polymorphism -757T/Cin CRP in the ICVD group (207/276(75.0％),65/276(23.6％),4/276(1.4％)) was significantly different from the control group (75/125 (60.0％),46/125 (36.8％),4/125 (3.2％) ; x2 =9.531,P =0.008),the frequence of T allele in patients with ICVD (479/552(86.8％)) was increased significantly compared to the control group (196/250 (78.4％)),the frequence of C allele in the ICVD group (73/552(13.2％)) was decreased significantly in contrast to the control group (54/250 (21.6％) ;x2 =9.056,P =0.004).Among the patients with ICVD,the level of serum C-reactive protein in patients with-757TC/CC genotype was elevated significantly than that with-757TC/CC ((4.2± 1.6) mg/L vs (2.7 ± 1.9) mg/L; t =5.900,P ＜ 0.01).Logistic regression analysis demonstrated that the-757C allele in CRP was related to the carotid artery atherosclerosis (OR =1.379,95％ CI 0.852-0.864,P =0.023).Conclusions The polymorphism-757T/C in CRP maybe related to the risk of developing ICVD.The frequency of the-757C allele can increase the level of serum C-reactive protein.And this polymorphism may be associated with carotid artery atheroselerosis in patients with ICVD.