中华神经科杂志
中華神經科雜誌
중화신경과잡지
Chinese Journal of Neurology
2013年
4期
265-268
,共4页
宋冲%楚亚楠%贺桂琼%刘刚%王凌唏%周泽芬%姚秋会
宋遲%楚亞楠%賀桂瓊%劉剛%王凌唏%週澤芬%姚鞦會
송충%초아남%하계경%류강%왕릉희%주택분%요추회
梓醇%淀粉样β蛋白前体%早老素1%小鼠,转基因%衰老斑%学习%记忆
梓醇%澱粉樣β蛋白前體%早老素1%小鼠,轉基因%衰老斑%學習%記憶
재순%정분양β단백전체%조로소1%소서,전기인%쇠로반%학습%기억
Catalpol%Amyloid beta-protein precursor%Presenilin-1%Mice,transgenic%Senile plaques%Learning%Memory
目的 观察梓醇对淀粉样蛋白前体、早老素1(APP/PSI)双转基因小鼠老年斑和学习记忆能力的影响.方法 将3个月龄的APP/PS1双转基因小鼠按照随机数字表法分为梓醇治疗组和生理盐水对照组,每组10只,并以10只同月龄的相同遗传背景的C57小鼠作为正常对照组.用梓醇(每天5 mg/kg)和等量生理盐水腹腔注射阿尔茨海默病(AD)模型小鼠3周,应用免疫组织化学检测各组小鼠老年斑数量的变化；应用Morris水迷宫检测小鼠空间学习记忆能力的变化.结果 免疫组织化学结果显示:与生理盐水对照组(6.0±0.6)比较,梓醇治疗组小鼠老年斑数量(个)明显减少(2.3±0.7,t=3.500,P=0.025).行为学结果显示:(1)在可视平台实验中,3组小鼠找到平台的平均潜伏期和搜索的平均路径差异无统计学意义.(2)隐蔽平台下,梓醇治疗组小鼠找到平台的时间及搜索的路径较生理盐水对照组小鼠明显缩短；与正常对照组比较,差异无统计学意义.(3)在探索实验中,60 s内梓醇治疗组(6.4±0.5)小鼠穿越平台次数(次)明显高于生理盐水对照组(2.9±0.4,t=5.592,P=0.001),而与正常对照组(6.8±0.6)比较差异无统计学意义(t=0.418,P=0.682).结论 梓醇治疗能显著减少AD模型小鼠脑内老年斑数量,改善小鼠的空间学习记忆能力.
目的 觀察梓醇對澱粉樣蛋白前體、早老素1(APP/PSI)雙轉基因小鼠老年斑和學習記憶能力的影響.方法 將3箇月齡的APP/PS1雙轉基因小鼠按照隨機數字錶法分為梓醇治療組和生理鹽水對照組,每組10隻,併以10隻同月齡的相同遺傳揹景的C57小鼠作為正常對照組.用梓醇(每天5 mg/kg)和等量生理鹽水腹腔註射阿爾茨海默病(AD)模型小鼠3週,應用免疫組織化學檢測各組小鼠老年斑數量的變化；應用Morris水迷宮檢測小鼠空間學習記憶能力的變化.結果 免疫組織化學結果顯示:與生理鹽水對照組(6.0±0.6)比較,梓醇治療組小鼠老年斑數量(箇)明顯減少(2.3±0.7,t=3.500,P=0.025).行為學結果顯示:(1)在可視平檯實驗中,3組小鼠找到平檯的平均潛伏期和搜索的平均路徑差異無統計學意義.(2)隱蔽平檯下,梓醇治療組小鼠找到平檯的時間及搜索的路徑較生理鹽水對照組小鼠明顯縮短；與正常對照組比較,差異無統計學意義.(3)在探索實驗中,60 s內梓醇治療組(6.4±0.5)小鼠穿越平檯次數(次)明顯高于生理鹽水對照組(2.9±0.4,t=5.592,P=0.001),而與正常對照組(6.8±0.6)比較差異無統計學意義(t=0.418,P=0.682).結論 梓醇治療能顯著減少AD模型小鼠腦內老年斑數量,改善小鼠的空間學習記憶能力.
목적 관찰재순대정분양단백전체、조로소1(APP/PSI)쌍전기인소서노년반화학습기억능력적영향.방법 장3개월령적APP/PS1쌍전기인소서안조수궤수자표법분위재순치료조화생리염수대조조,매조10지,병이10지동월령적상동유전배경적C57소서작위정상대조조.용재순(매천5 mg/kg)화등량생리염수복강주사아이자해묵병(AD)모형소서3주,응용면역조직화학검측각조소서노년반수량적변화；응용Morris수미궁검측소서공간학습기억능력적변화.결과 면역조직화학결과현시:여생리염수대조조(6.0±0.6)비교,재순치료조소서노년반수량(개)명현감소(2.3±0.7,t=3.500,P=0.025).행위학결과현시:(1)재가시평태실험중,3조소서조도평태적평균잠복기화수색적평균로경차이무통계학의의.(2)은폐평태하,재순치료조소서조도평태적시간급수색적로경교생리염수대조조소서명현축단；여정상대조조비교,차이무통계학의의.(3)재탐색실험중,60 s내재순치료조(6.4±0.5)소서천월평태차수(차)명현고우생리염수대조조(2.9±0.4,t=5.592,P=0.001),이여정상대조조(6.8±0.6)비교차이무통계학의의(t=0.418,P=0.682).결론 재순치료능현저감소AD모형소서뇌내노년반수량,개선소서적공간학습기억능력.
Objective To investigate whether catalpol affects senile plaque formation and spatial learning and memory ability in the amyloid-β protein precursor/presenilin 1 (APP/PSI) double transgenic mice.Methods Three month-old APP/PS1 double transgenic mice were randomly divided into catalpoltreated and saline-treated groups (n =10),with C57 mice of the same age and genetic background as normal control group (n =10).The catalpol (in a dose of 5 mg · kg-1 · d-1) and the same amount of saline were peritoneally injected into Alzheimer' s disease (AD) model mice for 3 weeks.Immunohistochemical staining was performed to examine senile plaques in the brain of AD model mice,and Morris water maze was used to assess the spatial learning and memory abilities of the mice.Results Compared with the saline-treated AD model mice (6.0 ±0.6),the number of senile plaques of catalpol treated AD mice significantly decreased (2.3± 0.7; t =3.500,P =0.025); Mice in each groups had similar latency and path length to reach platform in visible platform test; In hidden platform test,catalpol-treated mice had a significant lesser latency and path length compared with saline-treated mice,furthermore,catalpol-treated mice had much more platform-crossing times (6.4 ± 0.8) than saline-treated mice (2.9 ± 0.4 ; t =5.592,P =0.001).Conclusion Catalpol can significantly decrease the senile plaque formation and improve the spatial learning and memory abilities of APP/PS1 double transgenic mice.
