中华神经科杂志
中華神經科雜誌
중화신경과잡지
Chinese Journal of Neurology
2013年
11期
751-754
,共4页
胡潇方%吴世政%张淑坤%乌仁塔娜%白振忠
鬍瀟方%吳世政%張淑坤%烏仁塔娜%白振忠
호소방%오세정%장숙곤%오인탑나%백진충
缺血预处理%梗死,大脑中动脉%微RNAs%电子传递复合物Ⅳ%大鼠
缺血預處理%梗死,大腦中動脈%微RNAs%電子傳遞複閤物Ⅳ%大鼠
결혈예처리%경사,대뇌중동맥%미RNAs%전자전체복합물Ⅳ%대서
Ischemic preconditioning%Infarction,middle cerebral artery%MicroRNAs%Electron transport complex Ⅳ%Rats
目的 探讨miR-181c对缺氧预处理大鼠的神经保护作用及其作用机制.方法 将39只雄性SD大鼠按随机数字表法分成5组,分别为正常对照组、假手术组、单纯缺血组、缺氧预处理组、缺氧预处理缺血组.术后测定神经功能缺失体征评分、脑梗死面积；实时荧光定量PCR检测皮质中miR-181c的表达；蛋白质印迹检测皮质中线粒体细胞色素c氧化合酶1亚基(mt-cox1)蛋白的表达.结果 缺氧预处理缺血组大鼠的神经功能缺失体征较单纯缺血组大鼠有明显改善,同时梗死面积由22.50％±2.96％减小到16.40％ ±3.13％(t=5.26,P＜0.01).缺氧预处理缺血组miR-181c的表达较单纯缺血组表达明显降低(1.89±0.14与3.05±0.26,t=6.10,P＜0.01),其mt-cox1蛋白的表达也明显降低(0.54±0.07与0.93±0.04,t =8.01,P＜0.01).结论 缺氧预处理可能通过下调miR-181c基因表达使其靶蛋白mt-cox1表达增加,有效缓解SD大鼠的缺血损伤.
目的 探討miR-181c對缺氧預處理大鼠的神經保護作用及其作用機製.方法 將39隻雄性SD大鼠按隨機數字錶法分成5組,分彆為正常對照組、假手術組、單純缺血組、缺氧預處理組、缺氧預處理缺血組.術後測定神經功能缺失體徵評分、腦梗死麵積；實時熒光定量PCR檢測皮質中miR-181c的錶達；蛋白質印跡檢測皮質中線粒體細胞色素c氧化閤酶1亞基(mt-cox1)蛋白的錶達.結果 缺氧預處理缺血組大鼠的神經功能缺失體徵較單純缺血組大鼠有明顯改善,同時梗死麵積由22.50％±2.96％減小到16.40％ ±3.13％(t=5.26,P＜0.01).缺氧預處理缺血組miR-181c的錶達較單純缺血組錶達明顯降低(1.89±0.14與3.05±0.26,t=6.10,P＜0.01),其mt-cox1蛋白的錶達也明顯降低(0.54±0.07與0.93±0.04,t =8.01,P＜0.01).結論 缺氧預處理可能通過下調miR-181c基因錶達使其靶蛋白mt-cox1錶達增加,有效緩解SD大鼠的缺血損傷.
목적 탐토miR-181c대결양예처리대서적신경보호작용급기작용궤제.방법 장39지웅성SD대서안수궤수자표법분성5조,분별위정상대조조、가수술조、단순결혈조、결양예처리조、결양예처리결혈조.술후측정신경공능결실체정평분、뇌경사면적；실시형광정량PCR검측피질중miR-181c적표체；단백질인적검측피질중선립체세포색소c양화합매1아기(mt-cox1)단백적표체.결과 결양예처리결혈조대서적신경공능결실체정교단순결혈조대서유명현개선,동시경사면적유22.50％±2.96％감소도16.40％ ±3.13％(t=5.26,P＜0.01).결양예처리결혈조miR-181c적표체교단순결혈조표체명현강저(1.89±0.14여3.05±0.26,t=6.10,P＜0.01),기mt-cox1단백적표체야명현강저(0.54±0.07여0.93±0.04,t =8.01,P＜0.01).결론 결양예처리가능통과하조miR-181c기인표체사기파단백mt-cox1표체증가,유효완해SD대서적결혈손상.
Objective To investigate the neuroprotective effect of miR-181c on hypoxia-preconditioned ischemia in rats and its mechanism.Methods Thirty-nine male SD rats were randomly divided into 5 groups of control group,sham-operated group,middle cerebral artery occlusion (MCAO)group,hypoxia-preconditioned group,hypoxia-preconditioned and MCAO group.Infarct volume and behavioral deficits were quantified.Real-time PCR was applied to detect the expression levels of miR-181c and Western blotting was used to verify the target protein of mt-cox1.Results Under the treatment of hypoxia-preconditioned,the neurological impairment was alleviated and the infarct volume was reduced significantly from 22.50％ ±2.96％ to 16.40％ ±3.13 ％ (t =5.26,P ＜0.01).The expression of miR-181c was decreased significantly in hypoxia-preconditioned and MCAO group than that in MCAO group (1.89 ± 0.14 vs 3.05 ± 0.26,t =6.10,P ＜ 0.01),and the expression of mt-cox1 protein was also significantly decreased (0.54 ± 0.07 vs 0.93 ± 0.04,t =8.01,P ＜ 0.01).Conclusion Hypoxia-preconditioned may attenuate the ischemic injury in SD rats,which may be related to the down-regulation of the expression of miR-181c,therefore increasing the expression of its targeted protein mt-cox1.
