中华神经科杂志
中華神經科雜誌
중화신경과잡지
Chinese Journal of Neurology
2014年
9期
638-642
,共5页
偏头痛%三唑类%色胺类%三叉神经节%降钙素基因相关肽%脑啡肽类%蛋白质前体%胆囊收缩素
偏頭痛%三唑類%色胺類%三扠神經節%降鈣素基因相關肽%腦啡肽類%蛋白質前體%膽囊收縮素
편두통%삼서류%색알류%삼차신경절%강개소기인상관태%뇌배태류%단백질전체%담낭수축소
Migraine disorders%Triazoles%Tryptamines%Trigeminal ganglion%Calcitonin gene-related peptide%Enkephalins%Protein precursors%Cholecystokinin
目的 观察利扎曲普坦对偏头痛大鼠三叉神经节降钙素基因相关肽(CGRP)、脑啡肽原和缩胆囊肽基因表达的影响,探讨曲普坦类药物对偏头痛发作时三叉神经节功能的干预作用.方法 将24只健康Wistar大鼠按随机区组法分为4组:A组:对照组,B组:偏头痛组,C组:利扎曲普坦对照组,D组:利扎曲普坦治疗组.利扎曲普坦给药组(C、D组)大鼠给予利扎曲普坦1mg·kg-1·d-1灌胃,A组和B组大鼠给予生理盐水2 ml/d灌胃.上述处理7d后,B、D组大鼠制备硝酸甘油型偏头痛动物模型,造模2h留取三叉神经节标本,用实时定量PCR检测其CGRP、脑啡肽原和缩胆囊肽mRNA表达.结果 A、B、C、D各组大鼠三叉神经节每200纳克总RNA中CGRP mRNA拷贝数(×10 7)分别为:0.05±0.01、1.30±0.52、0.23 ±0.12、0.43 ±0.33;脑啡肽原mRNA拷贝数(×103)分别为:3.30±1.65、0.34±0.14、3.91±2.44、0.71±0.13.B组大鼠三叉神经节CGRP mRNA拷贝数明显高于A组(q =7.854,P＜0.05),D组大鼠三叉神经节CGRP mRNA拷贝数明显低于B组(q =5.458,P＜0.05);B组大鼠三叉神经节脑啡肽原mRNA拷贝数明显低于A组(q =4.478,P＜0.05);D组大鼠三叉神经节脑啡肽原mRNA拷贝数明显低于C组(q=4.838,P＜0.05);各组大鼠三叉神经节缩胆囊肽mRNA表达差异无统计学意义.结论 利扎曲普坦可以抑制偏头痛发作时三叉神经节CGRP的过度表达,进而减弱三叉神经血管系统的激活,发挥治疗偏头痛的作用;偏头痛发作时三叉神经节脑啡肽原的基因表达明显减少,使得偏头痛发作时三叉神经节内脑啡肽减少,减弱脑啡肽的镇痛效应,利于头痛的持续.
目的 觀察利扎麯普坦對偏頭痛大鼠三扠神經節降鈣素基因相關肽(CGRP)、腦啡肽原和縮膽囊肽基因錶達的影響,探討麯普坦類藥物對偏頭痛髮作時三扠神經節功能的榦預作用.方法 將24隻健康Wistar大鼠按隨機區組法分為4組:A組:對照組,B組:偏頭痛組,C組:利扎麯普坦對照組,D組:利扎麯普坦治療組.利扎麯普坦給藥組(C、D組)大鼠給予利扎麯普坦1mg·kg-1·d-1灌胃,A組和B組大鼠給予生理鹽水2 ml/d灌胃.上述處理7d後,B、D組大鼠製備硝痠甘油型偏頭痛動物模型,造模2h留取三扠神經節標本,用實時定量PCR檢測其CGRP、腦啡肽原和縮膽囊肽mRNA錶達.結果 A、B、C、D各組大鼠三扠神經節每200納剋總RNA中CGRP mRNA拷貝數(×10 7)分彆為:0.05±0.01、1.30±0.52、0.23 ±0.12、0.43 ±0.33;腦啡肽原mRNA拷貝數(×103)分彆為:3.30±1.65、0.34±0.14、3.91±2.44、0.71±0.13.B組大鼠三扠神經節CGRP mRNA拷貝數明顯高于A組(q =7.854,P＜0.05),D組大鼠三扠神經節CGRP mRNA拷貝數明顯低于B組(q =5.458,P＜0.05);B組大鼠三扠神經節腦啡肽原mRNA拷貝數明顯低于A組(q =4.478,P＜0.05);D組大鼠三扠神經節腦啡肽原mRNA拷貝數明顯低于C組(q=4.838,P＜0.05);各組大鼠三扠神經節縮膽囊肽mRNA錶達差異無統計學意義.結論 利扎麯普坦可以抑製偏頭痛髮作時三扠神經節CGRP的過度錶達,進而減弱三扠神經血管繫統的激活,髮揮治療偏頭痛的作用;偏頭痛髮作時三扠神經節腦啡肽原的基因錶達明顯減少,使得偏頭痛髮作時三扠神經節內腦啡肽減少,減弱腦啡肽的鎮痛效應,利于頭痛的持續.
목적 관찰리찰곡보탄대편두통대서삼차신경절강개소기인상관태(CGRP)、뇌배태원화축담낭태기인표체적영향,탐토곡보탄류약물대편두통발작시삼차신경절공능적간예작용.방법 장24지건강Wistar대서안수궤구조법분위4조:A조:대조조,B조:편두통조,C조:리찰곡보탄대조조,D조:리찰곡보탄치료조.리찰곡보탄급약조(C、D조)대서급여리찰곡보탄1mg·kg-1·d-1관위,A조화B조대서급여생리염수2 ml/d관위.상술처리7d후,B、D조대서제비초산감유형편두통동물모형,조모2h류취삼차신경절표본,용실시정량PCR검측기CGRP、뇌배태원화축담낭태mRNA표체.결과 A、B、C、D각조대서삼차신경절매200납극총RNA중CGRP mRNA고패수(×10 7)분별위:0.05±0.01、1.30±0.52、0.23 ±0.12、0.43 ±0.33;뇌배태원mRNA고패수(×103)분별위:3.30±1.65、0.34±0.14、3.91±2.44、0.71±0.13.B조대서삼차신경절CGRP mRNA고패수명현고우A조(q =7.854,P＜0.05),D조대서삼차신경절CGRP mRNA고패수명현저우B조(q =5.458,P＜0.05);B조대서삼차신경절뇌배태원mRNA고패수명현저우A조(q =4.478,P＜0.05);D조대서삼차신경절뇌배태원mRNA고패수명현저우C조(q=4.838,P＜0.05);각조대서삼차신경절축담낭태mRNA표체차이무통계학의의.결론 리찰곡보탄가이억제편두통발작시삼차신경절CGRP적과도표체,진이감약삼차신경혈관계통적격활,발휘치료편두통적작용;편두통발작시삼차신경절뇌배태원적기인표체명현감소,사득편두통발작시삼차신경절내뇌배태감소,감약뇌배태적진통효응,리우두통적지속.
Objective This study assesses the influence of rizatriptan on calcitonin gene-related peptide (CGRP),proenkephalin (PENK) and cholecystokinin (CCK) mRNA expressions in the trigeminal ganglia of a rat migraine model and investigates the possible mechanisms by which triptans treat migraine.Methods A total of 24 rats were randomly divided into four groups:normal control group (A),migraine model group(B),rizatriptan control group (C) and rizatriptan treatment group(D).Groups C and D were intragastrically perfused with rizatriptan,1 mg/kg per day.After 7 days,nitroglycerin was subcutaneously injected into the buttocks of the groups B and D to induce migraine.Two hours after nitroglycerin injection,the trigeminal ganglia was isolated.CGRP,PENK and CCK mRNA expressions in the trigeminal ganglia were determined using SYBR Green Ⅰ real-time quantitative PCR.Results The copy number of CGRP mRNA (× 107) in 200 ng total RNA of each group was 0.05 ±0.01,1.30 ±0.52,0.23 ±0.12,0.43 ±0.33 ; The copy number of PENK mRNA (× 103) in 200 ng total RNA of each group was 3.30 ± 1.65,0.34 ±0.14,3.91 ± 2.44,0.71 ± 0.13.The copy number of CGRP mRNA in the trigeminal ganglia of group B was significantly higher than that of group A (q =7.854,P ＜ 0.05) ; CGRP mRNA expressions were significantly lower in the trigeminal ganglia of rats in group D compared with group B (q =5.458,P ＜0.05).Compared with group A,PENK mRNA expressions in the trigeminal ganglia of rats were significantly lower in group B (q =4.478,P ＜ 0.05).PENK mRNA expressions were significantly higher in trigeminal ganglia of rats in group C compared with group D (q =4.838,P ＜ 0.05).CCK mRNA expression in trigeminal ganglia of rats was similar among groups.Conelusions Rizatriptan can decrease the expressions of CGRP in the trigeminal ganglia of the migraine rats and exhibits neurogenic inflammation triggered by CGRP.PENK expressions decrease in the trigeminal ganglia of the migraine rats,weaken the analgesic effects of enkephalin.
