CAJ | 학술논문

目的通过检测过氧化物酶体增殖物激活受体γ(PPARγ)在人垂体腺瘤组织中的表达,探讨其在垂体腺瘤中的相关机制.方法通过免疫组化法确定经手术切除的83例垂体腺瘤组织细胞来源;采用RT-PCR、适时定量PCR检测研究83例垂体腺瘤组织及6例正常垂体组织PPARγ的mRNA表达量,采用Western blot法检测组织PPARγ蛋白质表达水平,分析不同类型垂体腺瘤组织之间核酸及蛋白水平表达量的差异.结果 GH腺瘤17例、PRL腺瘤15例、ACTH腺瘤18例、多激素腺瘤(MCPAs)17例、无功能腺瘤(NFAs)16例及正常对照组6例;mRNA水平检测显示所有垂体腺瘤组织的表达量均高于正常对照组,其中GH腺瘤的表达量最高,与其他组比较P＜0.05;蛋白水平检测显示GH腺瘤、PRL腺瘤、ACTH腺瘤及MCPAs的表达量均高于正常对照组(P＜0.05),GH腺瘤的表达量最高,NFAs的表达量与正常对照组差异无统计学意义(P＞0.05).结论 PPARγ转录及蛋白表达水平在人GH、PRL、ACTH及MCPAs垂体腺瘤组织中高表达,该基因与垂体腺瘤有一定的相关性,而无功能垂体腺瘤在核酸水平的表达量增高,蛋白水平的表达量不高,可能与激素的分泌水平有关;在GH腺瘤中的表达量最高,且与其他组相比,差异有统计学意义(P＜0.05),说明该类肿瘤与PPARγ的关系最为密切,可能与生长激素能刺激PPARγ的表达有关.
목적 통과검측과양화물매체증식물격활수체γ(PPARγ)재인수체선류조직중적표체,탐토기재수체선류중적상관궤제.방법 통과면역조화법학정경수술절제적83례수체선류조직세포래원;채용RT-PCR、괄시정량PCR검측연구83례수체선류조직급6례정상수체조직PPARγ적mRNA표체량,채용Western blot법검측조직PPARγ단백질표체수평,분석불동류형수체선류조직지간핵산급단백수평표체량적차이.결과 GH선류17례、PRL선류15례、ACTH선류18례、다격소선류(MCPAs)17례、무공능선류(NFAs)16례급정상대조조6례;mRNA수평검측현시소유수체선류조직적표체량균고우정상대조조,기중GH선류적표체량최고,여기타조비교P＜0.05;단백수평검측현시GH선류、PRL선류、ACTH선류급MCPAs적표체량균고우정상대조조(P＜0.05),GH선류적표체량최고,NFAs적표체량여정상대조조차이무통계학의의(P＞0.05).결론 PPARγ전록급단백표체수평재인GH、PRL、ACTH급MCPAs수체선류조직중고표체,해기인여수체선류유일정적상관성,이무공능수체선류재핵산수평적표체량증고,단백수평적표체량불고,가능여격소적분비수평유관;재GH선류중적표체량최고,차여기타조상비,차이유통계학의의(P＜0.05),설명해류종류여PPARγ적관계최위밀절,가능여생장격소능자격PPARγ적표체유관.
Objective To prove the expression of PPARγ in human pituitary adenomas,and administrate that the expression among different tumors has significant difference.Method 83 cases of pituitary adenoma tissues and 6 normal pituitary tissues were divided into different groups by immunohischemieal according to the source of tissues and cells.The mRNA of PPARγ in 83 pituitary adenomas tissues and 6 normal pituitary tissues were determined by RT-PCR and quantitative real-time PCR,the protein of PPARγ were detected by western-blot.Result we have 89 samples,17 samples tested as GH secreting-adenomas,15 samples tested as PRL secreting-adenomas,18 samples tested as ACTH secreting-adenomas,17 samples tested as miscellaneous pituitary adenomas (MCPAs),16 samples tested as nonfunctional pituitary adenomas (NFAs) and 6 samples tested as normal;the PPARγ mRNA expressions were higher in all kinds of pituitary adenomas than in the normal pituitary tissue.GH secreting-adenomas was the highest,and the differentia was significant in statistics;the protein expression level of PPARγ in GH secreting,Prolactin secreting,ATCH secreting and MCPAs were higher than in the normal pituitary tissue (P＜0.05),and the expression in GH secreting-adenomas was the highest which has markedly differentia.The expression level of NFAs has no different compared with normal pituitary (P＞0.05).Conclusion We supposed that PPARγ is an important molecular target in pituitary adenoma cells,especially for GH secreting-adenomas.