中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2011年
11期
1113-1116
,共4页
杨军%胡业帅%常永凯%马顺昌%齐建发%陈素华%寸恩浩%于春江
楊軍%鬍業帥%常永凱%馬順昌%齊建髮%陳素華%吋恩浩%于春江
양군%호업수%상영개%마순창%제건발%진소화%촌은호%우춘강
芳烃受体影响蛋白%垂体瘤%基因突变%侵袭性%Knosp分级
芳烴受體影響蛋白%垂體瘤%基因突變%侵襲性%Knosp分級
방경수체영향단백%수체류%기인돌변%침습성%Knosp분급
AIP%Pituitary adenoma%Gene mutation%Invasive%Knosp grade
目的 初步探讨AIP基因突变与垂体瘤侵袭性的相关性.方法 根据Knosp分级标准筛选出侵袭性垂体瘤18例,非侵袭性垂体瘤16例.两组共34例垂体瘤标本分别行PCR和AIP基因6个外显子的DNA测序,根据测序比对结果,对两组样本中AIP基因的突变进行统计学分析.结果 18例侵袭性垂体瘤中有4例出现Exon4的突变,而16例非侵袭性垂体瘤中仅有1例出现突变,前者AIP基因突变率为22％,后者为6％.经卡方检验,两组样本中的突变率差异有统计学意义(P=0.001).突变位点是Exon 4序列中一处C突变为T的突变位点.结论 侵袭性垂体瘤中AIP基因的突变率要显著高于非侵袭性垂体瘸,表示AIP基因的突变与垂体瘤的侵袭性之间存在相关性；Exon 4序列中有一处C突变为T的突变位点,表示样品中位于Exon 4该位点的基因型为CT杂合型；AIP基因有可能成为诊断并治疗侵袭性垂体瘤的新靶点.
目的 初步探討AIP基因突變與垂體瘤侵襲性的相關性.方法 根據Knosp分級標準篩選齣侵襲性垂體瘤18例,非侵襲性垂體瘤16例.兩組共34例垂體瘤標本分彆行PCR和AIP基因6箇外顯子的DNA測序,根據測序比對結果,對兩組樣本中AIP基因的突變進行統計學分析.結果 18例侵襲性垂體瘤中有4例齣現Exon4的突變,而16例非侵襲性垂體瘤中僅有1例齣現突變,前者AIP基因突變率為22％,後者為6％.經卡方檢驗,兩組樣本中的突變率差異有統計學意義(P=0.001).突變位點是Exon 4序列中一處C突變為T的突變位點.結論 侵襲性垂體瘤中AIP基因的突變率要顯著高于非侵襲性垂體瘸,錶示AIP基因的突變與垂體瘤的侵襲性之間存在相關性；Exon 4序列中有一處C突變為T的突變位點,錶示樣品中位于Exon 4該位點的基因型為CT雜閤型；AIP基因有可能成為診斷併治療侵襲性垂體瘤的新靶點.
목적 초보탐토AIP기인돌변여수체류침습성적상관성.방법 근거Knosp분급표준사선출침습성수체류18례,비침습성수체류16례.량조공34례수체류표본분별행PCR화AIP기인6개외현자적DNA측서,근거측서비대결과,대량조양본중AIP기인적돌변진행통계학분석.결과 18례침습성수체류중유4례출현Exon4적돌변,이16례비침습성수체류중부유1례출현돌변,전자AIP기인돌변솔위22％,후자위6％.경잡방검험,량조양본중적돌변솔차이유통계학의의(P=0.001).돌변위점시Exon 4서렬중일처C돌변위T적돌변위점.결론 침습성수체류중AIP기인적돌변솔요현저고우비침습성수체가,표시AIP기인적돌변여수체류적침습성지간존재상관성；Exon 4서렬중유일처C돌변위T적돌변위점,표시양품중위우Exon 4해위점적기인형위CT잡합형；AIP기인유가능성위진단병치료침습성수체류적신파점.
Objective To analyze the relationship between the mutations of AIP gene and the aggressive ability of pituitary adenomas.Method The clinical data of 18 invasive pituitary adenomas and 16 non - invasive pituitary adenomas classified by Knosp criteria were collected.PCR and DNA sequence of six exons in AIP of all samples were performed.Results Through comparing the sequence investigated with subjects in Genebank,it was found that there were 4 of 18 (22％) specimens mutated in aggressive pituitary adenomas while there was 1 of 16( 6％ ) mutated in other samples.There was significantly difference between the two samples ( P =0.001 ).The mutation types were transferred from C to T.Conclusions There is higher mutation rate of AIP gene in aggressive pituitary tumors than that in non - invasive tumors.There is relationship between AlP mutations and aggression.In the mutation site,the base is transferred from C to T.It is suggested that the genotype is mixed of C and T.The relationship between AIP mutations and aggression need more samples to certify.AIP gene will be expected to become a new target point for early diagnosis and therapy of aggressive pituitary adenomas.
