中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2011年
12期
1272-1275
,共4页
Cx43蛋白表达%缝隙连接%内皮素%脑血管痉挛
Cx43蛋白錶達%縫隙連接%內皮素%腦血管痙攣
Cx43단백표체%봉극련접%내피소%뇌혈관경련
Connexin43%Gap junction%ET - 1%Cerebral vasospasm
目的 探讨Cx43在内皮素诱导的脑基底动脉收缩中的表达变化及其可能的作用.方法 血管环张力实验检测内皮素诱导的脑基底动脉的收缩变化并应用Western blot检测基底动脉Cx43蛋白的表达变化,染料传输实验用来检测脑基底动脉收缩过程中平滑肌细胞间缝隙连接的功能变化.结果 浓度递增的内皮素导致脑基底动脉呈显著浓度依赖性的收缩,一定浓度缝隙连接阻断剂苷珀酸显著缓解该收缩；收缩过程中,Cx43的蛋白表达呈显著时间依赖性的升高,苷珀酸减弱该表达的升高；内皮素刺激下,血管平滑肌细胞间的染料传输呈时间依赖性的升高,苷珀酸显著减少染料在细胞间的传输.结论 脑血管痉挛过程中,通过增加Cx43的表达,血管细胞间缝隙连接的功能被内皮素激活并在血管痉挛病理过程中发挥重要作用；抑制缝隙连接的功能是有效缓解蛛网膜下腔出血后脑血管痉挛的新途径.
目的 探討Cx43在內皮素誘導的腦基底動脈收縮中的錶達變化及其可能的作用.方法 血管環張力實驗檢測內皮素誘導的腦基底動脈的收縮變化併應用Western blot檢測基底動脈Cx43蛋白的錶達變化,染料傳輸實驗用來檢測腦基底動脈收縮過程中平滑肌細胞間縫隙連接的功能變化.結果 濃度遞增的內皮素導緻腦基底動脈呈顯著濃度依賴性的收縮,一定濃度縫隙連接阻斷劑苷珀痠顯著緩解該收縮；收縮過程中,Cx43的蛋白錶達呈顯著時間依賴性的升高,苷珀痠減弱該錶達的升高；內皮素刺激下,血管平滑肌細胞間的染料傳輸呈時間依賴性的升高,苷珀痠顯著減少染料在細胞間的傳輸.結論 腦血管痙攣過程中,通過增加Cx43的錶達,血管細胞間縫隙連接的功能被內皮素激活併在血管痙攣病理過程中髮揮重要作用；抑製縫隙連接的功能是有效緩解蛛網膜下腔齣血後腦血管痙攣的新途徑.
목적 탐토Cx43재내피소유도적뇌기저동맥수축중적표체변화급기가능적작용.방법 혈관배장력실험검측내피소유도적뇌기저동맥적수축변화병응용Western blot검측기저동맥Cx43단백적표체변화,염료전수실험용래검측뇌기저동맥수축과정중평활기세포간봉극련접적공능변화.결과 농도체증적내피소도치뇌기저동맥정현저농도의뢰성적수축,일정농도봉극련접조단제감박산현저완해해수축；수축과정중,Cx43적단백표체정현저시간의뢰성적승고,감박산감약해표체적승고；내피소자격하,혈관평활기세포간적염료전수정시간의뢰성적승고,감박산현저감소염료재세포간적전수.결론 뇌혈관경련과정중,통과증가Cx43적표체,혈관세포간봉극련접적공능피내피소격활병재혈관경련병리과정중발휘중요작용；억제봉극련접적공능시유효완해주망막하강출혈후뇌혈관경련적신도경.
Objective To investigate the role of connexin43 in ET- 1 -induced contraction in rabbit basilar artery.Methods The ET - 1 - induced contraction without or with carbenoxolone was studied with an isometric tension system.The expression of connexin43 protein in ET - t stimulated basilar arteries was studied with Western blot.Scrape/scratch method was used to analyze the function of gap junction in cultured rabbit cerebrovascular smooth muscle ceils.Results ET - 1 produced a concentration - dependent contraction.Carbenoxolone inhibited ET- 1 induced contraction.The connexin43 protein level was increased in ET- 1stimulated basilar arteries.Carbenoxolone decreased the connexin43 protein level increased by ET - 1.Cells treated with ET - 1 appeared positive communication and the dye transfer was increased in a time - dependent fashion.Carbenoxolone suppressed the ET - 1 - induced increasement of dye transfer.Conclusions The enhancement of gap junction intercellular communication is activated by ET - 1 via modulating the expression of connexin43,and plays an important role in the pathogenesis of cerebral vasospasm.Inhibition of vascular GJIC as a means of reducing cerebral vasospasm after SAH may have therapeutic advantage.
