CAJ | 학술논문

目的探索人骨髓基质细胞( hBMSCs)静脉移植治疗脑出血的实验效果及其可能机制.方法选用成年雄性Wistar大鼠80只,随机分为对照组及3个移植不同数量(1×106/ml,3×106/ml,6×106/ml)细胞的治疗组.经尾静脉注射hBMSCs治疗大鼠脑出血.计算分析神经学缺陷程度分数、尾状核组织损失的百分率以及TUJ1 、BrdU、mAb1281、突触素免疫组化结果.结果脑出血后14d,3个治疗组的神经功能明显改善(P＜0.05)；尾状核组织损失面积明显减小(P＜0.05)；TUJ1、BrdU、突触素的阳性细胞染色面积明显增加(P＜0.05)；mAb1281阳性染色细胞大量出现在脑出血区(P＜0.05).结论经尾静脉移植hBMSCs,能明显改善脑出血大鼠的神经功能,这与脑出血区尾状核组织损失较小、神经元及神经突触再生有关.
목적 탐색인골수기질세포( hBMSCs)정맥이식치료뇌출혈적실험효과급기가능궤제.방법 선용성년웅성Wistar대서80지,수궤분위대조조급3개이식불동수량(1×106/ml,3×106/ml,6×106/ml)세포적치료조.경미정맥주사hBMSCs치료대서뇌출혈.계산분석신경학결함정도분수、미상핵조직손실적백분솔이급TUJ1 、BrdU、mAb1281、돌촉소면역조화결과.결과 뇌출혈후14d,3개치료조적신경공능명현개선(P＜0.05)；미상핵조직손실면적명현감소(P＜0.05)；TUJ1、BrdU、돌촉소적양성세포염색면적명현증가(P＜0.05)；mAb1281양성염색세포대량출현재뇌출혈구(P＜0.05).결론 경미정맥이식hBMSCs,능명현개선뇌출혈대서적신경공능,저여뇌출혈구미상핵조직손실교소、신경원급신경돌촉재생유관.
Objective To investigate the therapeutic effects and possible mechanism of intravenous administration of human bone marrow stromal cells (hBMSCs) in the treatment of intracerebral hemorrhage in rats.Methods Eighty adult male Wistar rats were randomly divided into four experimental groups,which consisted of 3 treatment groups and 1 control group.The intracerebral hemorrhage (ICH) in rats was treated by injecting hBMSCs into the rats＇ tail veins.The neurological function was evaluated using NSS.The percentage of striatal tissue loss in one section was calculated using an image analysis system.All rats were immunohistochemically analyzed using TUJ1,mAb1281,BrdU and Synaptophysin.Statistical evaluation of functional scores,area of ICH - related tissue damage,and histochemical results were performed using independent Student t - test- Results There was apparent difference between the control group and any of the treatment groups in the results of NSS 14 day after ICH( P ＜0.05).There was virtually difference between the control group and any of the treatment groups in the area of striatal tissue loss as a percentage of the normal hemisphere 2 weeks after ICH( P ＜0.05).The BrdU,Synaptophysin and TUJ1 histochemical staining data suggested that there was significant increase in the positive -staining cells in the region of ICH for all treatment groups compared with the controls ( P ＜ 0.05 ).Labeling of mAb1281 was seen in the treated animals in the region of ICH( P ＜0.05),verifying that the injected hBMSCs did reach the site of injured brain preferentially compared with the contralateral hemisphere.Conclusions Intravenous injection of hBMSCs at doses of 1,3 and 6 million cells 14 day after experimental ICH improves neurological function,and is associated with reduced striatal tissue loss,increased local presence of hBMSCs at the site of hemorrhage,significant increases in mitotic activity,immature neurons,synaptogenesis.