CAJ | 학술논문

目的高岭土诱导Wistar大鼠脑积水后系统监测大鼠体质量、颅内压、脑室面积和胼胝体厚度的改变.方法雄性Wistar大鼠60只,按随机数字表顺序分为实验组(n＝40)和对照组(n=20).实验组40只大鼠再随机分为A、B、C、D、E五个亚组,向枕大池内注入25％无菌高岭土混悬液0.1 ml;对照组注入等量无菌生理盐水,注入后3d、1周、2周、4周和8周后行体质量、MRI检查及颅内压监测,经视交叉切片测量胼胝体厚度.结果实验组共有23只大鼠脑室不同程度的扩大,侧脑室随时间延长逐渐扩大;脑积水大鼠体质量明显低于对照组;颅内压在高岭土注入后3d明显增高,7d左右达峰值,8周时颅内压有所下降,但仍明显高于正常值;胼胝体厚度随脑室扩张逐渐变薄.结论高岭土注入枕大池可成功诱导建立大鼠交通性脑积水模型,脑积水后大鼠体质量、颅内压、脑室扩张和胼胝体厚度呈一系列连续的变化,不同时间窗各有特点.
목적 고령토유도Wistar대서뇌적수후계통감측대서체질량、로내압、뇌실면적화변지체후도적개변.방법 웅성Wistar대서60지,안수궤수자표순서분위실험조(n＝40)화대조조(n=20).실험조40지대서재수궤분위A、B、C、D、E오개아조,향침대지내주입25％무균고령토혼현액0.1 ml;대조조주입등량무균생리염수,주입후3d、1주、2주、4주화8주후행체질량、MRI검사급로내압감측,경시교차절편측량변지체후도.결과 실험조공유23지대서뇌실불동정도적확대,측뇌실수시간연장축점확대;뇌적수대서체질량명현저우대조조;로내압재고령토주입후3d명현증고,7d좌우체봉치,8주시로내압유소하강,단잉명현고우정상치;변지체후도수뇌실확장축점변박.결론 고령토주입침대지가성공유도건립대서교통성뇌적수모형,뇌적수후대서체질량、로내압、뇌실확장화변지체후도정일계렬련속적변화,불동시간창각유특점.
Objective To develop models of kaolin - induced hydrocephalus in adult Wistar rats and to measure changes of body weight,intracranial pressure,ventriculomegaly and corpus callosum thickness.Methods 60 adult male Wistar rats were randomly divided into experimental group and control group.In the former group,40 rats were randomly separated into 5 subgroups:group A,B,C,D and E,then were used to construct animal models of hydrocephalus by injecting 0.1 ml volume of 25％ kaolin sterile solution into the cisterna Magana with the aid of a surgical microscope.Controls were trcatcd in the same way with injection of saline.Body weighing,MRI and ICP were performed at the tine point of 3 days,1,2,4 and 8 weeks after kaolin injection,respectively.Corpus callosum thickness was measured at the level of the optic chiasm.Results Hydrocephalus was induced in 23 rats whose ventricular system expauded as times went on.In hydrocephalus rats,body weight was significantly lower than in age - matched saline - injected sham controls,ICP increased rapidly on day 3 post intracisternal kaolin injection,reached a maximum on day 7,remained markedly elevated through 8 week, and had reduced corpus callosum thickness as ventricle enlargment.Conclusions Kaolin injection through cistern magna can effectively eatablish a rat model of communicating hydrocephalus.Aherations in ICP,ventriculomegaly and corpus callosum thickness occurs sequentially,and the time - course of each manifestation of hydrocephalus differs.