中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2012年
11期
1172-1175
,共4页
刘爽%张剑宁%尹丰%郭欣茹%王淑为%张宽%范文红%范明
劉爽%張劍寧%尹豐%郭訢茹%王淑為%張寬%範文紅%範明
류상%장검저%윤봉%곽흔여%왕숙위%장관%범문홍%범명
胶质母细胞瘤%脑肿瘤干细胞%神经干细胞%趋向性
膠質母細胞瘤%腦腫瘤榦細胞%神經榦細胞%趨嚮性
효질모세포류%뇌종류간세포%신경간세포%추향성
Glioblastoma%Brain tumor stem cells%Human neural stem cells%Tropism
目的 探讨人神经干细胞(hNSCs)对脑肿瘤干细胞(BTSCs)的趋向性.方法 利用神经球培养方法培养胶质母细胞瘤(GBM)来源的BTSCs.采用Transwell细胞迁移实验检测hNSCs对BTSCs的体外趋向性;利用立体定向注射的方法,建立裸鼠颅内AAV-EGFP-BTSCs移植瘤模型,将标记有红色荧光蛋白(RFP)的人源性NSCs(hNSCs)接种到移植瘤对侧脑组织,荧光显微镜观察hNSCs向移植瘤的迁移情况及在移植瘤体内的分布.结果 GBM组织中可分离扩增到CD133+和Nestin+的BTSCs细胞,BTSCs在体外以神经球的形式生长,将这些细胞接种到裸鼠颅内可形成新的胶质瘤.细胞迁移实验显示体外培养的hNSCs可向BTSCs定向迁移,裸鼠颅内移植瘤实验显示hNSCs可在颅内沿切线向BTSCs移植瘤定向迁移,并能穿透肿瘤组织,到达瘤组织中心区.结论 外源hNSCs对BTSCs具有明确的体内外趋向性.这一特性可被用于干细胞为载体的基因治疗研究.
目的 探討人神經榦細胞(hNSCs)對腦腫瘤榦細胞(BTSCs)的趨嚮性.方法 利用神經毬培養方法培養膠質母細胞瘤(GBM)來源的BTSCs.採用Transwell細胞遷移實驗檢測hNSCs對BTSCs的體外趨嚮性;利用立體定嚮註射的方法,建立裸鼠顱內AAV-EGFP-BTSCs移植瘤模型,將標記有紅色熒光蛋白(RFP)的人源性NSCs(hNSCs)接種到移植瘤對側腦組織,熒光顯微鏡觀察hNSCs嚮移植瘤的遷移情況及在移植瘤體內的分佈.結果 GBM組織中可分離擴增到CD133+和Nestin+的BTSCs細胞,BTSCs在體外以神經毬的形式生長,將這些細胞接種到裸鼠顱內可形成新的膠質瘤.細胞遷移實驗顯示體外培養的hNSCs可嚮BTSCs定嚮遷移,裸鼠顱內移植瘤實驗顯示hNSCs可在顱內沿切線嚮BTSCs移植瘤定嚮遷移,併能穿透腫瘤組織,到達瘤組織中心區.結論 外源hNSCs對BTSCs具有明確的體內外趨嚮性.這一特性可被用于榦細胞為載體的基因治療研究.
목적 탐토인신경간세포(hNSCs)대뇌종류간세포(BTSCs)적추향성.방법 이용신경구배양방법배양효질모세포류(GBM)래원적BTSCs.채용Transwell세포천이실험검측hNSCs대BTSCs적체외추향성;이용입체정향주사적방법,건립라서로내AAV-EGFP-BTSCs이식류모형,장표기유홍색형광단백(RFP)적인원성NSCs(hNSCs)접충도이식류대측뇌조직,형광현미경관찰hNSCs향이식류적천이정황급재이식류체내적분포.결과 GBM조직중가분리확증도CD133+화Nestin+적BTSCs세포,BTSCs재체외이신경구적형식생장,장저사세포접충도라서로내가형성신적효질류.세포천이실험현시체외배양적hNSCs가향BTSCs정향천이,라서로내이식류실험현시hNSCs가재로내연절선향BTSCs이식류정향천이,병능천투종류조직,도체류조직중심구.결론 외원hNSCs대BTSCs구유명학적체내외추향성.저일특성가피용우간세포위재체적기인치료연구.
Objective To clarify the capacity of selective migration of human neural stem cells (hNSCs) to brain tumor stem cells (BTSCs) derived from glioblastoma in vitro and vivo.Methods The BTSCs were cultured from GBM tissues by Neurosphere culture method and the tropism ability of hNSCs to BTSCs were investigated using in vitro transwell chamber cell migration assay and in vivo tumor tropism studies.Results The BTSCs could be obtained from human GBM tissues,and formed neuroshpheres after cultured 7 days in vitro,and were CD133-positive and nestin-positive.Theses BTSCs could regenerate a GBM tumor in vivo when implanted intraventricularly.Cell migration assay showed that the BTSCs had the capacity to actively attract hNSCs.The fate of hNSCs in vivo could be visualized under confocal fluorescence microscope.The hNSCs exhibited extensive tropism toward the site of the tumor and infiltrate tumor foci.Conclusions The BTSCs could be derived rapidly from GBM tissues by neurosphere method.The normal hNSCs possesse the capacity to migrate toward BTSCs in vitro and vivo.The tropism of hNSCs towards brain tumors may provide an additional tool for the treatment of brain cancer.
